This study was carried out in order to produce experimental infection and investigate the life cycle of Linguatula serrata in dogs. Nymphs of Linguatula serrata were isolated from infected hepatic and lung tissues of sheep, cattle and buffalo. Isolated parasites were fed to four dogs of two months of age. The eggs of Linguatula serrata were isolated by weekly sampling in month of infection. 7, 7, 5 and 2 parasites were isolated from infected dogs. Nasal discharge itching and mild respiratory difficulties were recorded in the infected dogs.