There is increasing demand for use of natural preservatives such as bacteriocins to control foodborne pathogens in seafoods. This study was performed to evaluate the antimicrobial effects of Nisin on Staphylococcus aureus in light and heavy salted silver carp fillets (4 and 8% Nacl). Effect of different concentrations of Nisin (0, 0.15, 0.25, 0.75, 1.5 µg/ml) on behavior of S.aureus at unfavourable refrigeration storage temperature (10oC) was determined by evaluation of the bacterial growth in salted fish fillets. The results showed that used concentrations of Nisin had inhibitory effect on the growth of S.aureus in light and heavy salted fish fillets compared to the control (p<0.05). This study showed inhibitory effect of Nisin on the growth of S.aureus. Therefore it can be considered as a natural preservative in light and heavy salted fish.