Journal of Veterinary Research

Journal of Veterinary Research

STUDYON THE CYTOTOXICITYLEVELOF NANOSILVER ON NATURALFIBROBLAST(HF2), OSTEOSARCOMA(G292) AND MESENCHYMALSTEM CELLS

Authors
delavar shahbazzadeh
Hamed Ahari
Mehdi Soltani
Hamid Rashedi
Mohammad Reza Shokrgozar
Somayeh Moaddab
Mozhgan Heidarpour
Jafar Rahman-Nia
Abstract
Nanotechnology offers numerous opportunities for invention of new and reformed applicable products for the benefit of human society. In spite of a vast application of nanomaterials there is little information about their impact on human health. This study examines the biological activity of nanosilver on mesenchymal, natural fibroblast (HF2), and osteoblast (G292) cells. The effects of nanosilver on the cells were observed by a light microscope and the amplification of the cells was assayed by using a standard cell toxicity test. The results show that the cytotoxicity depends on nanosilver concentration. The amount of IC50 on mesenchymal stem was 6.33; and on HF2 was 6.68; and on G292 cells was 3.42 ?g/L. The results show that nanosilver has two times more
of an inhibition effect on cancerous cells' growth as compared to the normal cells. This phenomenon is due to the direct effect of nanosilver on the cell oxidation system. Due to the extraordinary activation of the mitochondorial respiration system in cancer cells, when compared to the normal cells, it can provide suitable opportunity for nanosilver to cause cell disruption.
Keywords
Cytotoxicity
fibroblast
Mesenchymal stem cells
nanosilver
osteoblast
Journal of Veterinary Research
Volume 66, Issue 4 - Serial Number 4
Autumn 2011
Pages 305-308