Journal of Veterinary Research

Journal of Veterinary Research

THE EFFECTOFMICROENCAPSULATION WITH CALCIUM ALGINATE AND RESISTANTSTARCH ON THE LACTOBACILLUS ACIDOPHILUS (LA5) SURVIVAL RATE IN SIMULATED GASTROINTESTINAL JUICE CONDITIONS

Authors
Hadi pourjafar
Aziz Homayouni Rad
Abstract
Microencapsulation of probiotic cells is the newest method for increasing probiotics' survival
in probiotic food products in gastrointestinal conditions and safely transferring them to the large
intestine. The aim of this study was to investigate the effect of microencapsulation with calcium
alginate and resistant starch on the lactobacillus acidophilus (La5), and the survival rate in
simulated gastrointestinal juice conditions. Survivability of this bacteria was conducted in 0.6%
bile salt solution and simulated gastric juice(PH1.55), followed by incubation in simulated
intestinal juice with and without 0.6% bile salt. In this study the extrusion method was performed
for the microencapsulation process. Data were analyzed using an independent t-test. Our results
showed that survivability of microencapsulated cells in a simulated gastric juice condition, and
also in simulated intestinal juice with and without 0.6% bile salt, is significantly more than free
cells(p<0/05).
Keywords
calcium alginate
gastrointestinal juice
lactobacillus acidophilus
Microencapsulation
resistant starch
Journal of Veterinary Research
Volume 66, Issue 4 - Serial Number 4
Autumn 2011
Pages 337-342