Study on P53 gene alterations (5 and 6 exons) in bovine urinary bladder tumors

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BACKGROUNDS: P53 is a tumor suppressive gene which frequently mutates in tumors of animals and human. This gene commonly mutates in urinary bladder tumors of human beings. Urinary bladder tumors have occurred in cattle with bovine enzootic hematuria (BEH). OBJECTIVES: The aim of present study was to evaluate P53 mutations in 15 samples of different bovine urinary bladder tumors by the PCR-SSCP technique. METHODS: Fifteen paraffin embedded blocks were selected from different kinds of bovine urinary bladder tumors. DNA was extracted from the samples and PCR was done by using specified primers for 5 and 6 exons. After electrophoresis, the PCR products were assessed by the SSCP method, and samples with changes in electrophoresis patterns were selected and sequenced. RESULTS: Results showed that there are intronic alterations of the P53 gene in cattle with urinary bladder tumors. There were no changes in the electrophoretic pattern of exons 5 and 6, but on each side of the designed primers for exon 6, there was a part of introns 5 and 6. The samples, including hemangioma, papilloma and carcinoma in situ with electrophoretic changes, showed nucleotide T deletion with number 9332 in intron 6 after direct sequencing. Intronic mutations can be a predisposition for developing cancers. CONCLUSIONS: It is possible that some of urinary tumors are inducted by P53 mutations in intronic zone.

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