MOLECULAR STUDIES ON AVIAN LEUKOSIS VIRUS SUBGROUP J (ALV-J) INFECTIONS IN GRANDPARENT FLOCKS IN IRAN

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Abstract:
Subgroup J Avian Leukosis Virus (ALV-J) was isolated in the late 1980s from meat-type chickens in the United Kingdom. ALV-J with exception of some acute variants, cause myeloid leukosis in meat-type chickens with long incubation period.In this study while the status of Avian Leukosis Virus Subgroup J in six different strain of broiler grandparent flocks of Iran evaluated, the evaluation of different primers and some molecular methods also were done. For this reason, 100 blood samples, which had EDTA, 100 mixed of white blood cells and plasma and 100 feather pulps were collected from one farm of each broiler grandparent strain. PCR and nested PCR were methods that used for the study. In molecular study before the extraction of DNA from samples; In order to decrease the number of samples, 10 mixed of white blood cells and plasma, blood or feather pulp pooled. In molecular study with PCR all of samples were negative, but in nested PCR reaction, from six broiler strains, five strains infected to ALV-J. The results indicate that the most of broiler strains in Iran have been infected to ALV-J. The results also indicate that we can simply use pooled samples for the study of ALV-J in a flock by nested-PCR test.
Key words: broiler grandparent, ALV-J, PCR, nested-PCR.

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