Detection of somatic antigens in Mycobacterium avium subspecies paratuberculosis



Bakground : Johne's disease or Paratuberculosis as a chronic granulomatosis enteritis in ruminants will be caused by Mycobacerium avium subsp . Paratuberculosis . Detecting whole bacterial cell wall antigens would be helpful in potential applications for diagnosis , vaccine production , and elucidation of pathogenesis . Objectives : To determine secreted somatic cell antigens of Mycobacterium avium subspecies Paratuberculosis . Methods : Standard strain (III-V) of Mycobacterium avium subspecies Paratuberculosis DNA was extracted from the cultured and gene analysis was done using PCR to confirm bacterial purity . On the other hand , protein concentrations in both media and cell entracts were determined . Furthermore , all proteins pattern were shown by SDS -PAGE . Results : Electrophoretic findings showed some somatic antigens in the range of 19 - 100 KDa. Conclusions : These purified somatic antigens can be used for further study and potential application in vaccine production .