Detection of somatic antigens in Mycobacterium avium subspecies paratuberculosis

Madani, Rasol; Azadeh, Shabnam; Mosavari, Nader; Jafarpour, Mostafa; Golchinfar, Fariba; Keshavarz, Ruhollah; Emami, Tara

doi:10.22059/jvr.2012.29301

Detection of somatic antigens in Mycobacterium avium subspecies paratuberculosis

Authors

10.22059/jvr.2012.29301

Abstract

Bakground : Johne's disease or Paratuberculosis as a chronic granulomatosis enteritis in ruminants will be caused by Mycobacerium avium subsp . Paratuberculosis . Detecting whole bacterial cell wall antigens would be helpful in potential applications for diagnosis , vaccine production , and elucidation of pathogenesis . Objectives : To determine secreted somatic cell antigens of Mycobacterium avium subspecies Paratuberculosis . Methods : Standard strain (III-V) of Mycobacterium avium subspecies Paratuberculosis DNA was extracted from the cultured and gene analysis was done using PCR to confirm bacterial purity . On the other hand , protein concentrations in both media and cell entracts were determined . Furthermore , all proteins pattern were shown by SDS -PAGE . Results : Electrophoretic findings showed some somatic antigens in the range of 19 - 100 KDa. Conclusions : These purified somatic antigens can be used for further study and potential application in vaccine production .

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