Immunoinformatic survey of Boophilus tick tropomyosin protein

Authors

1 Department of Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran- Iran

2 Department of Parasitology and Tick and Tick Born Diseases center, Faculty of Veterinary Medicine, University of Tehran, Tehran- Iran

3 Rastegar Central Laboratory, Faculty of Veterinary Medicine, University of Tehran, Tehran- Iran

4 Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran- Iran

5 Faculty of Veterinary Medicine, Amol University of Special Modern Technology, Amol-Iran

Abstract

BACKGROUND: Boophilus tick is responsible for the transmission of some pathogens (types of germs) and the reduction of fertility in Cattles. Tropomyosin protein plays an important role in regulating actin function, immune and allergic reactions and vaccine production. OBJECTIVES: Accurate identification of antigenic determinants (epitopes) is difficult, time consuming and not economic, thus we focused on development of rapid methods such as prediction based on sequence and structure of protein. METHODS: A wide array of Immunoinformatics softwares and tools were used for the analysis of antigenicity, frequency in protein secondary structure and domains, physic-chemical factors related to region of linear epitopes, B cell epitopes, and binding peptide segments to MHC II alleles in cattle. RESULTS: Protein analysis with different algorithms and evaluated accuracy of our result with different profiles of protein results in 3 selected epitopes (AA62-80(NH2-EQLSQANSKLEEKDKALQA-COOH), AA170-185(NH2-AMVEADLERAEERAETG-COOH) and AA200-218 (NH2-VGNNLKSLEVSEEKALQKEET-COOH)) for B cell and a epitope AA192-207(NH2-LEEELRVVGNNLKSL-COOH) for T cell. CONCLUSIONS: For desirable stimulation of immune system, only some segments of protein (epitopes) are enough. Selecting efficient epitopes causes concentration immune responses, preparation suitable vaccine and reduces allergic and adverse responses.
 

Keywords

Journal of Veterinary Research
Volume 69, Issue 4 - Serial Number 4
December 2014
Pages 335-345

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