Phylogenetic analysis comparing partial S1 gene of avian infectious bronchitis virus to commercial vaccine strains in Iran

Authors

1 Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Karaj-Iran

2 Departments of Poulty Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran

3 Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Karaj-Iran.

4 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran

Abstract

BACKGROUND: Infectious bronchitis is a highly contagious disease which may cause poor weight gain and low feed efficiency in infected chickens. There are a large number of reported serotypes/genotypes, which makes the control of the disease more difficult through vaccination. However, there are only a few amino acid differences in the S1 protein of vaccine and wild type strains which are responsible for protection. OBJECTIVES: The purpose of this study is to compare IBV variants isolated from commercial chicken flocks in Iran with currently used vaccine strains. METHODES: The partial S1 gene of the spike protein, covering a hypervariable and constant regions, was amplified and sequenced using conventional RT-PCR. RESULTS: Phylogenetic analysis of amino acid sequences revealed that eight of total nine isolates were divergence at least 21.8% from vaccinal Massachusetts serotypes, and six of nine isolates were divergence at least 22.7% from 4/91, and none of the nine isolates were similar to Dutch-type, D274,vaccine serotypes. CONCLUSIONS: These findings are essential for continuous surveillance disease control strategies and monitoring of variants, and thus emphasize on the importance of improving the vaccination program in Iran.

Keywords

References

Abdel-Moneim, A.S., El-Kady, M.F., Ladman, B.S., Gelb, J. (2006) S1gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in Egypt. Virol J. 3: 78-82.
Adzhar, A., Gough, R.E., Haydon, D., Shaw, K., Britton, P., Cavanagh, D. (1997) Molecular analysis of the 793/B serotype of infectious bronchitis virus in Great Britain. Avian Pathol. 26: 625–640.
Aghakhan, S.M., Abshar, N.,  Rasoul Nejad, F.,  Marunesi, C.,  Khodashenas, M. (1994) Studies on avian viral infections in Iran. Arch Inst Razi. 44/45: 1-10.
Ahmed, Z.,  Naeem, K.,  Hameed, A. (2007) Detection and seroprevalence of infectious bronchitis virus strains in commercial poultry in Pakistan.  Poultry Sci. 86: 1329-1335.
Akbari Azad, G., Vasfi Marandi, M., Keyvani Aminae, H. (2007) Molecular analysis of three Iranian isolates belonged to 793/B serotype of infectious bronchitis.  J Vet Res. 62: 69-80.
Bijlenga, G.,  Cook, J., Gelb, J., de Wit, J.J. (2004) Development and use of the H strain of avian infectious bronchitis virus from the Netherlands as a vaccine: a review. Avian Pathol.  33: 550-557.
Calogero, T., Toffan, A., Serena Beato, M., De Nardi, R., Vascellari, M., Meini, A., Ortali, G., Marzia Mancin., Ilaria Capua. (2008) Pathogenicity of a QX strain of infectious bronchitis virus in specific pathogen free and commercial broiler chickens, and evaluation of protection induced by a vaccination programme based on the Ma5 and 4/91 serotypes. Avian Pathol. 37: 487-493.
Capua, I., Minta, Z., Kaepinska, E.,  Mawditt, K.,  Britton, P., Cavanagh, D., Gough, R.E. (1999)  Co-circulation of four types of infectious bronchitis virus (793/B, 624/I, B1648 and Massachusetts). Avian Pathol. 28: 587-592.
Cavanagh, D., Naqi, S.A. (2008) Infectious Bronchitis. Diseases of Poultry. (12th ed.) Blackwell Publishing Professional, Ames, Iowa  USA.
Cavanagh, D., Picaul, J.P., Gough, R.E., Hess, M., Mawditt, K., Britton, P. (2005) Variation in the spike protein of the 793/B type of infectious bronchitis virus, in the field and during alternate passage in chickens and embryonated eggs. Avian Pathol.  34: 20-25.
Cook, J., Chester, J., Baxendale, N., Greenwood, N., Huggins, M.B., Orbell, S.J. (2001) Protection of chickens against renal damage caused by nephro-pathogenic infectious bronchitis virus. Avian Pathol. 30: 423-426.
Cook, J.K.A., Orbell, S.J., Woods, M.A., Huggins, M.B. (1999) Breadth of protection of the respiratory tract provided by different live-attenuated infectious bronchitis vaccines against challenge with infectious bronchitis viruses of heterologous serotypes. Avian Pathol. 28: 477-485.
Cook, J.K.A., Williams Smith, H., Huggins, M.B. (1986) Infectious bronchitis immunity: its study in chickens experimentally infected with mixtures of infectious bronchitis virus and Escherichia coli. J Gen Virol. 67: 1427-1434.
Ghahremani, N., Bozorgmehri Fard, M.H., Shoushtari, H., Momayez, R., Sheikhi, N., Khoshzahmat, A., Eshratabadi, F. (2011)  Molecular analysis of infectious bronchitis viruses isolated in Iran from 1998-2008. J Anim Vet Adv. 10: 2961-2967.
Haqshenas, G., Assasi, K., Akrami, H. (2005) Isolation and molecular characterization of infectious bronchitis virus isolate Shiraz 3. IBV, by RT-PCR and restriction enzyme analysis. Iran J Vet Res, University of Shiraz. 6: 9-15.
Hashemzadeh, M., Karimi,V., Masoudi, Sh.  Shoushtary, A.H., Ghalyanchi Langeroudi, A., Momayez, R., Nazem Shirazi, M.H., Maghsodloo, H., Hasanzadeh, R., Eshratabadi, F. (2013) Phylogenetic study of Iranian infectious bronchitis virus isolates during 2010-2011 using glycoprotein S1 gene. J Vet Res. 68:135-140.
Liu, S., Kong, X. (2004) A new genotype of nephro-pathogenic infectious bronchitis virus circulating in vaccinated and nonvaccinated flocks in China. Avian Pathol. 33: 321-327.
Mahdavi, S.,Tavasoly, A., Pourbaksh, S.A., Momayez, R. (2007) Experimental histopathologic study of the lesions induced by serotype 793/B (4/91) infectious bronchitis virus. Arch Razi Inst. 62: 15-22.
Matthijs, M.G.R., Van Eck, J.H.H., Landman, W.J.M., Stegeman, J.A. (2003) Ability of Massachusetts-type infectious bronchitis virus to increase colibacillosis susceptibility in commercial broilers: a comparison between vaccine and virulent field virus. Avian Pathol. 32: 473-481.
Meir, R., Rosenblut, E., Perl, S., Kass, N., Ayali, G., Hemsani, E., Perk, S. (2004) Identification of a novel nephropathogenic infectious bronchitis virus in Israel. Avian Dis. 48: 635–641.
Momayez, R., Pourbakhsh, S.A., Khodashenas, M., Banani, M. (2002) Isolation and   identification of infectious bronchitis virus from commercial chickens. Arch Razi Inst. 53: 1-9.
Nouri, A., Assasi, K., Seyfi Abad Shoupouri, M.R. (2003) Field study of infectious bronchitis virus using. Type specific RT-PCR. Arch Razi Inst. 55: 1-10.
Parsons, D., Ellis, M.M., Cavanagh, D., Cook, J.K.A. (1992) Characterization of an infectious bronchitis virus isolated from vaccinated broiler breeder flocks. Vet Rec. 131: 408-411.
Rosenberger, J.K., Alphin, R.L., Krauss, W.S. (1976)  Cross-protection studies with a Holland strain of IBV. Avian Dis. 20: 199-201.
Seifi, S., Asasi, K., Mohammadi, A. (2010)  Natural co-infection caused by avian influenza H9 subtype and infectious bronchitis viruses in broiler chicken farms. Vet Archiv. 80: 269-281.
Seify abad Shapori, M.R, Mayahi, M., Charkhkar, S., Assasi, k. (2002) Serotype identification of recent Iranian isolates of infectious bronchitis virus by type-specific multiplex RT-PCR. Arch Razi Inst. 5: 79- 85.
Shoushtari, A.H., Toroghi, R., Momayez, R., Pourbakhsh, S.A. (2008) 793/B type, the Predomin-ant circulating type of Avian Infectious Bronchitis Viruses 1999 - 2004 in Iran: A retrospective study. Arch Razi Inst. 63: 1-5.
Smith, H.W., Cook, J.K.A., Parsell, Z.E. (1985) The experimental infection of chickens with mixtures of infectious bronchitis virus and Escherichia coli. J Gen Virol. 66: 777-786.
Timms, L.M., Bracewell, C.D. (1981) Cell mediated and humeral immune response of chickens to live infectious bronchitis vaccines. Res Vet Sci. 31: 182-189.
Toro, H., Godoy, V., Larenas, J., Reyes, E., Kaleta, E.F. (1996) Avian infectious bronchitis: viral persistence in the Harderian gland and histological changes after eyedrop vaccination. Avian Dis. 40: 114-120.
Vasfi Marandi, M., Bozogmehri Fard, M.H. (2001) Isolation and identification of infectious    bronchitis viruses in chickens between 1997-2000 in Iran. J Vet Res. 56: 119-124.
Winterfield, R.W., Fadly, A.M. (1975) Potential for polyvalent infectious bronchitis vaccines. Am J Vet Res. 36: 524-526.
Winterfield, R.W., Fadly, A.M., Hoerr, F.J. (1976) Immunity to infectious bronchitis virus from spray vaccination with derivatives of a Holland strain. Avian Dis. 20: 42-48.
Winterfield, R.W., Fadly, A.M., Hoerr, F.J. (1976) Vaccination and revaccination with a Holland (H) strain of infectious bronchitis virus. Avian Dis. 20: 369-374.
Zana, H.M.,Rizgar, R.S., Aumaid, U.U.  (2011) Isolation and molecular characterization of sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East.Vet Microbiol. 150: 21-27.

Files

Share

How to cite

Statistics