Evaluation of anti-Candida properties of cationic peptides derived from Rana ridibunda skin


1 Academic Center of Education, Culture and Research (ACECR), Tehran-Iran; Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran

2 Faculty of Veterinary Medicine, Amol University of special Modern Technologies, Amol-Iean

3 Department of Toxicology, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran

4 Academic Center of Education, Culture and Research (ACECR), Tehran-Iran

5 Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran

6 Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran


BACKGROUND: Antimicrobial peptides with relative length (2-100 amino acids) and positive charge (pure charge (+9)-(+2)) are amphiphilic that isolated from a wide range of animals. Recently, these peptides have been known as a part of innate immune response. Nowadays, more than 500 antimicrobial peptides from animals have been reported. Objectives: The aim of this study was to evaluate the anti-Candida effects of cationic peptides derived from Rana ridibunda skin. Methods: In this study, using alcohol-acid technique, peptides of frog’s skin were isolated and purified by Sep-Pack and Sephadex column. Then the anti-Candida activity (Fluconazole Resistance C. albicans, Fluconazole Sensitive C. albicans, and C. glabrata) of the peptides in different concentrations were evaluated. Results: Regarding to statistical analysis, peptides in concentration ranging from 25 to 100 µg/ml had the most anti-Candida activities. In respect to different understudy Candida species, these agents had the less effect on the Fluconazole Resistance C. albicans (p<0.05). Conclusions: The anti-Candida effects of cationic peptide obtained from frog skin are approved in this study.


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