Determination of growth performance, changes of carcass composition and blood parameters in common carp fingerlings (Cyprinus carpio) fed with different levels of vitamin E


1 Department of Fisheries, Talesh Branch, Islamic Azad University, Talesh-Iran

2 Department of Fisheries, Rasht Branch, Islamic Azad University, Rasht-Iran

3 National Inland Water Aquaculture Institute, Bandar Anzali-Iran


BACKGROUND: Vitamins are considered an essential part in diet of aquatic animals. OBJECTIVES: This study was carried out to assay the effect of different levels of vitamin E on growth performance, carcass compositions and blood parameters of common carp fingerlings (Cyprinus carpio). METHODS: Total number of 96 common carp fingerlings weighing 15.49 ± 3.64 g were randomly distributed into 12 fiberglass tanks in four treatments group including control (without added vitamin), 100, 200 and 400 mg/kg vitamin E in three replicates and kept at a density of 8 fish per tank for 10 weeks. RESULTS: Results showed that the highest weight gain, percentage of body weight increase, mean daily growth, specific growth rate, condition factor and the lowest food conversation ratio were observed in fish fed with 200mg/kg vitamin E but no significant differences were seen compared to other treatments (p>0.05). Fish fed with 200mg/kg vitamin E had the highest (p>0.05) contents of protein and ash compared to control group. There was an increase in values of RBC, Hct and Hb in fish fed with 100, 200 and 400 mg/kg vitamin E in comparison with control group. Significant differences were observed in MCHC in fish fed with 100 mg/kg vitamin E compared to control and fish fed with 400 mg/kg, also treatment 200 mg/kg with treatment 400 mg/kg vitamin E (p<0.05). Fish fed vitamin E in 200 and 100 mg/kg had the highest WBC count. Fish fed with different levels of vitamin E had higher lymphocyte in comparison with control group and fish fed with 100 mg/kg had significant difference with control in lymphocyte (p<0.05). CONCLUSIONS: Vitamin E in level of 200 mg/kg can play an important role to enhance growth performance, nutrition efficiency and improving blood indices and support cell immunity in common carp fingerlings.


Abdel-Hameid, N., Abidi, S.H., Khan, M.A. (2011) Dietary vitamin E requirement for maximizing the growth, conversion efficiency, biochemical composition and haematological status of fingerling Channa punctatus. Aquac Res. 43: 226-338.##
AOAC, (Association of Official Analytical Chemists). (1995) Official Methods of Analysis of AOAC International. (16th ed.) AOAC International, Arlington, Virginia, USA.##
Benfey, T.G., Biron, M. (2000) Acute strees response in triploid rainbow trout (Oncorhynchus mykiss) and book trout (Salvelinus fontinalis). Aquaculture. 184: 167-176.##
Dixon, B., Stet, R.J.M. (2001) The relationship between major histocompatibility receptors and innate immunity in teleost fish. Dev Comp Immunol. 25: 683-700.##
Falahatkar, B., Soltani, M., Abtahi, B., Kalbassi, M.R., Pourkazemi, M., Yasemi, M. (2007) Effects of vitamin C on some growth parameters, survival and hepatosomatic index in juvenile cultured beluga, Huso huso. J Pajouhesh and Sazandegi. (In Persian). 72: 98-103.##
Falahatkar, B., Abdi, H., Mahmoudi, N. (2012) The role of dietary nucleotide on energy sources and growth function of common carp, Cyprinus carpio. Iran Sci Fish J. 21: 133-146.##
Faridpak, F. (2007) Manual Guide to: Spawning and Culture of Warmwater Fishes. (2nd ed.) Abzian Scientific Press. Tehran, Iran. ##
Genc, M.A., Aktas, M., Genc, E., Yilmaz, E. (2007) Effects of dietary mannan oligosaccharide on growth, body composition and hepatopancreas histology of Penaeus semisulcatus. Aquacult Nutr. 13: 156-161.##
Guillaume, J., Kaushik, S., Bergot, P., Metailler, R. (2001) Nutrition and Feeding of Fish and Crustaceans. (1st ed.) Springer press. London, UK. ##
Halver, J.E., Hardy, R.W. (2002) Fish Nutrition.  (3rd ed.) Academic Press, San Diego, California, USA. ##
Hamre, K. (2011) Metabolism, interactions, requirments and functions of vitamin E in fish. Aquacult Nutr. 17: 98-115.##
Hanaee Kashani, Z., Imanpoor, M.R., Shabani, A., Gorgin, S. (2012) The effect of vitamin E and highly unsaturated fatty acid on growth and some biochemical blood parameters of gold fish (Carassius auratus gibelio). J Utiliz Cultiv Aquat. 1: 15-26.##
Huang, C.H., Chang, R.J., Huang, S.L., Chen, W. (2003) Dietary vitamin E supplementation affects tissue lipid peroxidation of hybrid tilapia, Oreochromis niloticus × O. aureus. Comp Biochem Physiol. 134: 265-270.##
Huang, C.H., Higgs, D.A., Balfry, S.K., Devlin, R.H. (2004) Effect of dietary vitamin E level on growth, tissue lipid peroxidation, and erythrocyte fragility of transgenic Coho salmon, Oncorhynchus kisutch. Comp Biochem Physiol. 139: 199- 204.##
Keiffer, J.D. (2000) Limits to exhaustive exercise in fish. Comp Biochem Physiol. 126: 161-179.##
Klontz, G.W. (1994) Fish hematology. In: Techniques in Fish Immunology. Stolen, J.S., Fletcher, T.C., Rowley, A.F., Kelikoff, T.C., Kaatari, S.L. and Smith, S.A. (eds.). (2nd ed.) Vol. 3. SOS Publications, Fair Haven, New Jersey, USA. p.121-132.##
Lim, C., Yildirim-Aksoy, M., Li, M.H., Welker, T.L., Klesius, P.H. (2009) Influence of dietary levels of lipid and vitamin E on growth and resistance of Nile tilapia to Streptococcus iniae challenge. Aquaculture. 298: 76-82.##
Lin, Y.H., Shiau, S.Y. (2005) Dietary vitamin E requirement of grouper, Epinephelus malabaricus, at two lipid levels, and their effects on immune responses. Aquaculture. 248: 235- 244. ##
Luo, G., Xu, J., Teng, Y., Ding, C., Yan, B. (2010) Effects of dietary lipid levels on the growth, digestive enzyme, feed utilization and fatty acid composition of Japanese sea bass (Lateolabrax  japonicus) reared in freshwater. Aquac Res. 41: 210-219.##
Nakagawa , H ., Sato, M., Gatlin , D.M. (2007) Dietary  Supplements  for the Health and Quality of Cultured Fish. (1st ed.) CABI Publishing. New York, USA. ##
Paul, B.N., Sarkar, S., Mohanty, S.N. (2004) Dietary vitamin E requirement of mrigal, Cirrhinus mrigala fry. Aquaculture. 242: 529-536.##
Rahimi, M., Ouraji, H. (2014) Effects of vitamin E on growth performance, survival rate, hematological parameters response to heat stress in rainbow trout (Oncorhynchus mykiss) at two stocking densities. J Aquac Feed Sci Nutr. 6: 39-46.##
Safarpour Amlashi, A., Falahatkar, B., Sattari, M., Tolouei Gilani, M.H. (2011) Effect of dietary vitamin E on growth, muscle composition, hematological and immunological parameters of sub-yearling beluga Huso huso (L.). Fish Shellfish Immun. 30: 807-814.##
Safarpour Amlashi, A., Falahatkar, B., Sharifi, S.D. (2012) Dietary vitamin E requirements and growth performance of young-of-the-year beluga, Huso huso (L.) (Chondrostei: Acipenseridae). Arch Pol Fish. 20: 299-306.##
Soltani, M. (2007) Fish and shellfish immunology. University of Tehran Press. Tehran, Iran.##
Staykov, Y., Spring, P., Denev, S., Sweetman, J. (2007) Effect of a mannan oligosaccharide on the growth performance and immune status of rainbow trout (Oncorhynchus mykiss). Aqua Int. 15: 153-161.##
Torrecillas, S., Makol, A., Caballero, M.J., Montero, D., Robaina, L., Real, F., Sweetman, L., Tort, M., Izquierdo, S. (2007) Immune stimulation and improved infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides. Fish Shellfish Immunol. 23: 969-981.##
Vossoughi, G.H., Mostajeer, B. (2010) Freshwater Fishes. (8th ed.) Tehran university press. Tehran, Iran.##
Webster, C.D., Lim, C. (2002) Nutrient Requirements and Feeding of Finfish for Aquaculture.  (1st ed.) CABI Publishing. New York, USA. ##
Winton, J.R. (2001) Fish health management. In:  Fish Hatchery Management. Wedemeyer, G. (ed). (2nd ed.) American Fisheries Society. Bethesda, Maryland, USA. p. 559-639.##