The effect of Anethum graveolens on egg quality, some blood metabolites and hatchability in broiler breeder hens


1 Department of Animal Sciences, Faculty of Agriculture, University of Guilan, Rasht- Iran

2 Department of Animal Sciences, Sari Agricultural Sciences and Natural Resources University, Sari- Iran

3 Department of Biochemistry and Nutrition, Kashan University of Medical Sciences, Kashan- Iran


BACKGROUND: Improvement of hatchability in aging broiler
breeder has economic justification. OBJECTIVES: The main objective of
this study was to determine the effect of dietary Anethum graveolens
essential oil on egg quality, some plasma metabolites and hatchability in
broiler breeder hens. METHODS: A total number of eighty hens and eight
cocks from Ross 308 strain of 105 weeks old were divided into four groups with
two replicates within each (10hens+ 1cock). The diets were supplemented with
out essential oil (Control) and with 10, 20 and 40 ml essential oil/100kg diets
for four weeks. RESULTS: There were no significant differences among the
groups in Haugh unit and yolk index. While, there were significant differences
in egg weight, egg shape index, shell thickness, shell ratio, yolk color and
shell weight. Plasma triglyceride, high density lipoprotein cholesterol (HDL-C)
and egg yolk cholesterol concentration were not influenced by dietary essential
oil of Anethum graveolens. Whereas essential oil increased plasma
cholesterol significantly, it also significantly decreased plasma glucose.
Hatchability and fertility were positively affected by supplementation of
essential oil in the diet. CONCLUSIONS: Results of this study showed
that supplementing diet with  Anethum
essential oil improved hatchability of total eggs set of broiler


Abd  El motaal, A.M., Ahmed,  A.M.H., Bahakaim,  A.S.A.,  Fathi,  M.M. (2008)  Productive performance and immunocompetence of commercial laying hens given diets supplemented with eucalyptus. Int J Poult Sci. 7: 445-449.
Ali, M.N., Hassan, M.S., Abd El-Ghany, F.A. (2007) Effect of strain, type of natural antioxidant and sulphate ion on productive, physiological and hatching performance of native laying hens. Int J Poult Sci. 6: 539-554.
Bagir, N.M., Aziza, Y.H., Rania, M.H., Ahmed, A.R., Anton, C.B. (2006) Lipid composition of egg yolk and serum in laying hens fed diets containing Black Cumin (Nigella Sativa). Int J Poult Sci. 6: 574-578.
Bolukbasi,  S.C., Erhan,  M.K., Kaynar,  O. (2008) The  effect of  feeding  thyme,  sage  and rosemary oil on laying hen performance, cholesterol and some proteins ratio of egg yolk and E. coli count in feces. Arch Fu Gef. 72: 231-237.
Bopanna, K.N., Kannan, J., Gadgil, S., Balaraman, E.R., Rathore, S.P. (1997) Antidiabetic and antihyperglycaemic effects of neem seeds kernel powder on alloxan diabetic rabbits. Indian. J Pharmacol. 29: 162-167.
Bozkurt, M., Alcicek, A., Cabuk, M., Kucukyilmaz, K., Catli, A.U. (2009) Effect of an herbal essential oil mixture on growth, laying traits, and egg hatching characteristics of broiler breeders. Poult Sci. 88: 2368-2374.
Braun, E.J., Sweazea, K.L. (2008) Glucose regulation in birds. Comparative Biochemistry and Physiology. Elsevier. 151: 1-9.
Canogullary, S., Karaman, M., Erdogan, Z., Baylan, M., Kucukgul, A., Duzguner V., Ozugur, A. K. (2009) Effect of garlic powder on egg yolk and serum cholesterol and performance of laying hens. Bull Vet Inst Pulawy. 53: 515-519.
Case, G.L., He, L., Mo, H., Elson, C.E. (1995) Induction of geranyl pyrophosphate pyrophosphatase activity by cholesterol-suppressive isoprenoids. J Lip. 30: 357-359.
Christensen, V. L. (2001) Factors associated with early embryonic mortality. World’s Poult. Sci J. 57: 359-372.
Christensen, V.L., Wineland, M.J., Fasenko, G.M., Donaldson, W.E. (2001) Egg storage effects on plasma glucose and supply and demand glycogen concentrations of broiler embryos. Poult Sci. 80: 1729-1735.
Dikmen, Y., Sahan, U. (2006) Correlations between breeder age, egg cholesterol content, blood cholesterol level and hatchability of broiler breeders. Br Poult Sci. 48: 98-103.
Ekpo, K.E, Azeke, M.A. (2008) Egg yolk cholesterol lowering effects of garlic and tea. J Biol Sci. 8: 456-460.
Feher, G. (1984) Development of the albumen sac and changes of its structure in the goose during hatching. Magy. Allatorvosok. Lapja. 39: 105-112.
Florou-Paneri, P., Nikolakakis, I., Giannenas, I., Koidis, A., Botsoglou, E., Dota, V., Mitsopoulos, I. (2005) Hen performance and egg quality as affected by dietary oregano essential oil and  a-tocopheryl  acetate  supplementation. Poult Sci. 4: 449-454.
Folch, J., Lees, M., Sloane-Stanly, G.H. (1957) A simple method for the isolation and purification of total lipids from animal tissue. J Biol Chem. 226: 477-509.
Galobart, J., Barroeta, A.C., Baucells, M.D., Codony, R., Ternes, W. (2001) Effect of dietary supplementation with rosemary extract and alpha tocopheryl acetate on lipid oxidation in eggs enriched with omega - 3 - fatty acids. Poult Sci. 80: 460- 467.
Hajhashemi, V., Abbasi, N. (2008) Hypolipidemic activity of Anethum graveolens in rats. Phytother Res. 22: 372-375.
Jirovetz, L., Buchbauer, G., Stoyanova, A.S. (2003) Composition, quality control and antimicrobial activity of the essential oil of long-time stored dill (Anethum graveolens L.) seeds from Bulgaria. J Agric Food Chem. 51: 3854-3857.
John, T.M., George, J.C., Moran, E.T. (1987) Pre- and posthatch ultrastructural and metabolic changes in the hatching muscle of turkey embryos from antibiotic and glucose treated eggs. Cytobios. 49: 197-210.
Khanna, K., Rizvi, F., Chander, R. (2002) Lipid lowering activity of Phyllanthu niruri in hyperlipedemic rats. J Ethnopharm. 82: 19-22.
Kucera, P., Raddatz, E., Baroffio, A. (1984) Oxygen and glucose uptake in the early chick embryo. Respiration and Metabolism of Embryonic Vertebrates. 3: 299-309.
Lemhadri, A., Hajji, L., Michel, J.B., Eddouks, M. (2006) Cholesterol and triglycerides lowering activities of caraway fruits in normal and streptozotocin diabetic rats. J Ethnopharmacol. 106: 321-326.
Radwan, N., Hassan, R.A., Qota, E.M., Fayek, H.M. (2008) Effect of natural antioxidant on oxidative stability of eggs and productive and reproductive performance of laying hens. Int J Poult Sci. 2: 134-150.
Safaa, H.M.( 2007) Effect Of dietary Garlic or Fenugreek on cholesterol metabolism in laying hen. Egypt Poult Sci. 27: 1207-122.
Sato, M., Tachibana, T., Furuse, M. (2006) Heat production and lipid metabolism in broiler and layer chickens during embryonic development. Comp Biochem Physiol. 143: 382-388.
Senanayake, G.V., Maruyama, M., Sakono, M., Fukuda, N., Morishita, T., Yukizaki, C., Kawano, M., Ohta, H. (2004) The effects of bitter melon (Momordica charantia) extracts on serum and liver lipid parameters in hamsters fed cholesterol-free and cholesterol-enriched diets. J Nutr Sci Vitaminol. 50: 4253-4257.
Speake, B.K., Murray, A.M.B., Noble, R.C. (1998) Transport and transformation of yolk lipids during development of the avian embryo. Prog Lipi Res. 37: 29-32.
Surai, A.P., Surai, P.F., Steinberg, W., Wakeman, W.G., Speake, B.K., Sparks, N.H.C. (2003) Effect of canthaxanthin content of the maternal diet on the antioxidant system of developing chick. Br Poult Sci. 44: 612-619.
Surai, P.F. (1999) Tissue-specific changes in the activities of antioxidant enzymes during the development of the chicken embryo. Br Poult Sci. 40: 397-405.
Tona, K., Bruggeman,V., Onagbesan, O., Bamelis, F., Gbeassor, M., Mertens, K., Decuypere, E. (2005) Day-old chick quality. Relationship of hatching egg quality, adequate incubation practice and prediction of broiler performance. Avian Poult Biol Rev. 16: 109-119.
Villamor, E., Ruijtenbeek, K., Pulgar, V., De Mey, J.G., Blanko, C.E. (2002) Vascular reactivity in intrapulmonary arteries of chicken embryos during transition to ex ovo life. Am. J Physiol Regul Integr Comp Physiol. 282: 917-927.
Whittow, G.C., Tarazawa, H. (1991) The early development of thermoregulation in birds. Physiol Zool. 64: 1371-1390.
Williams, P., Losa, R. (2001) The use of essential oils and their compounds in poultry nutrition. World’s Poult Sci. 17: 14-15.
Yazdanparast, R., Alavi, M. (2001) Antihyperlipidemic and antihypercholesterolemic effects of Anethum graveolens leaves after removal of furocoumarins. Cytobios. 105: 189-191.
Zheng, G.Q., Kenney, P.M., Lam, L.K. (1992) Anethofuran, carvone, and limonene. Potential cancer chemopreventive agents from dill weed oil and caraway oil. Planta Med. 58: 338-341.
Zlakis, A., Zak, B., Boyle, A. (1953) A new method for the direct determination of serum cholesterol. J Lab Clin Medic. 4: 486-492.