The effect of using Lactococcus lactis JF831150 on the status of the intestinal bacterial flora of Persian sturgeon (Acipenser persicus) and exposure to Aeromonas hydrophila

Authors

1 Department of Aquatic Animal Health, International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht- Iran

2 Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Center of excellence of aquatic animal health, University of Tehran- Iran

3 Iranian Fisheries Science Research Instititue

Abstract

BACKGROUND: Lactococcus lactis is one of the lactic acid bacteria that can be considered as a probiotic in Persian sturgeon. OBJECTIVES: The aim of the present study was to increase the resistance to Aeromonas hydrophila after feeding the L. lactis JF831150. METHODS: In the present study, first 60 fish were fed different doses of L.lactis JF831150 for 56 days. The bacterial flora were counted with  TSA and MRS. Resistance of fish against A. hydrophila were considered after feeding. RESULTS: The use of L. lactis JF831150 for 56 days in P. sturgeon showed significant improvement in decreasing heterotrophic aerobic bacteria flora and increasing the lactic acid bacteria in the intestine. In the face of fish against A. hydrophila in third treatment (108 CFU/g), the survival rate was shown to be significantly higher than the control and other treatments. CONCLUSIONS: The results showed that consumption of L. lactis JF831150 (108 CFU/g) could improve the intestinal flora and increase resistance to A. hydrophila, and therefore has  potential for use  with P. sturgeon.

Keywords


Abd El-Rhman, A.M., Khattab, Y.A.E. , Shalaby, A.M.E. (2009) Micrococcus luteus and Pseudomonas species as probiotics for promoting the growth performance and health of Nile tilapia, Oreochromis niloticus. Fish Shellfish Immun. 27:175-180.
Aly, S.M., Ahmed, YA-G., Ghareeb, AA-A.,Mohamed, M.F. (2008) Studies on Bacillus subtilis and Lactobacillus acidophilus, as potential probiotics, on the immune response and resistance of Tilapia nilotica (Oreochromis niloticus) to challenge infections. Fish Shellfish Immun. 25: 128-136.
Askarian, F., Kousha, A., RingØ, E. (2009) Isolation of lactic acid bacteria from the gastrointestinal tract of Beluga (Huso huso) and Persian sturgeon (Acipenser persicus). J Appl Ichthyol. 25 (Suppl. 2): 91-94.
Askarian, F., Kousha, A., Salma,W., Ringø, E.(2011) The effect of lactic acid bacteria administration on growth, digestive enzyme activity and gut microbiota in Persian sturgeon (Acipenser persicus) and beluga (Huso huso) fry. Aquacult Nutr. 17: 488-497.
Babu, S.M., Banerjee, C.S., Abraham, T.J. (2003) Effect of gram positive bacterium, Lactobacillus sp. on the growth performance of gold fish, Carassius auratus Linnaeus, 1758. Environ Ecol. 21: 17-19.
Balcazar, J.L., Blas, I., Ruiz-Zarzuela, I., Cunningham, D., Vendrell, D., Muzquiz, J.L. (2006) Review.The role of probiotics in aquaculture. Vet Microbiol. 114: 173-186.
Balcazar, J.L., Blas, I., Ruiz-Zazuela, I., Vandrell, D., Girones, O., Muzquiz, J.L. (2007) Enhancement of the immune response and protection induced by probiotic lactic acid bacteria against furunculosis in rainbow trout (Oncorhynchus mykiss). FEMS Immunol Med Mic. 51: 185-193.
Balcázar,  J.L., Vendrell, D., Blas, I., Ruiz-Zarzuela, I., Muzquiz, J.L., Girones, O. (2008) Characterization of probiotic properties of lactic acid bacteria isolated from intestinal microbiota of fish. Aquaculture. 278: 188-191.
Brunt, J., Newaj-Fyzul, A., Austin, B. (2007)The development of probiotics for the control of multiple bacterial diseases of rainbow trout, Oncorhynchus mykiss (Walbaum). J Fish Dis. 30: 573-579.
Castex, M., Lemaire, P., Wabete, N., Chimet, L. (2009) Effect of dietary probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress status of shrimp Litopenaeus stylirostris. Aquaculture. 294: 306-313. 
Díaz-Rosales, P., Arijo, S., Chabrillón,  M., Alarcón, F.J., Tapia-Paniagua, S.T., Martínez-Manzanares, E., Balebona, M.C., Moriñigo, M.A. (2009) Effects of two closely related probiotics on respiratory burst activity of Senegalese sole (Solea senegalensis, Kaup) phagocytes, and protection against Photobacterium damselae subsp. piscicida. Aquaculture. 293: 16-21.
Fooks, L.J., Fuller, R., Gibson, G.R. (1999) Prebiotics, probiotics and human gut microbiology. Int Dairy J. 9: 53-61.
Ghanbari, M., Rezaei, M., Jami, M., Nazari, R. M. (2009) Isolation and characterization of Lactobacillus species from intestinal contents of beluga (Huso huso) and Persian sturgeon (Acipenser persicus). Iran J Vet Res Shiraz Uni. 10: 152-157.
Hedrick, R.P., McDowell, T.S., Ahne, W., Torhy, C., Kinkelin, P. (1992) Properties of three  iridovirus-like agents associated with systemic infections of fish. Dis Aquat Oragan. 13: 203-209.
Khoshbavar-Rostami, H.A., Soltani, M., Hassan, H.M.D. (2006) Immune response of great sturgeon (Huso huso) subjected to long-term exposure to sublethal concentration of the organophosphate, diazinon. Aquaculture. 256: 88-94.
Khoshbavar-Rostami, H.A., Soltani, M., Hassan, H.M.D. (2007) Immune responses of great Sturgeon (Huso huso) to Aeromonas hydrophila. J Fish  Biol. 70: 1931-1938.
Kim, D., Austin, B. (2006) Innate immune responses in rainbow trout (Oncorhynchus mykiss, Walbaum) induced by probiotics. Fish  Shellfish Immun. 21: 513-524.
LaPatra, S.E., Groff, J.M., Jones, G.R., Munn, B., Patterson, T.L., Holt, R.A., Hauck, A.K.,  Hedrick, R.P. (1994) Occurrence of white sturgeon iridovirus infections among cultured white sturgeon in the Pacific Northwest. Aquaculture. 126: 201-210.
Meng,Y., Xiao, H.B., Zeng, L.B. (2011) Isolation and identification of the hemorrhagic septicemia pathogen from Amur sturgeon, Acipenser schrenckii. J Appl Ichthyol. 27: 799-803.
Merrifield, D.L., Dimitroglou, A., Bradley, G., Baker, R.T.M., Davies, S.J. (2009a) Probiotic applications for rainbow trout (Oncorhynchus mykiss Walbaum) I. Effects on growth performance, feed utilization, intestinal microbiota and related health criteria. Aquacult  Nutr. DOI: 10.1111/j.1365-2095.2009. 00689.x.
Merrifield, D.L., Bradley, G., Baker, R.T.M., Davies, S.J. (2009b) Probiotic applications for rainbow trout (Oncorhynchus mykiss Walbaum) II. Effects on growth performance, feed utilization, intestinal microbiota and related health criteria post antibiotic treatment. Aquacult  Nutr. DOI: 10.1111/j.1365-2095.2009. 00688.x
Merrifield, D.L., Bradley, G., Harper, G.M., Baker, R.T.M., Munn, C.B., Davies S.J. (2011) Assessment of the effects of vegetative and lyophilized Pediococcus acidilactici on growth, feed utilization, intestinal colonization and health parameters of rainbow trout (Oncorhynchus mykiss Walbaum). Aquacult  Nutr. 17: 73-79.
Nikoskelainen, S., Ouwehand, A., Salminen, S., Bylund, G. (2001) Protection of rainbow trout (Oncorhynchus mykiss) from furunculosis by Lactobacillus rhamnosus. Aquaculture. 198: 229-236.
Pan, X., Wu, T., Song, Z., Tang, H., Zhao, Z. (2008) Immune responses and enhanced disease resistance in Chinese drum, Miichthys miiuy (Basilewsky), after oral administration of live or dead cells of Clostridium butyricum CB2. J Fish  Dis. 31: 679-686.
Rengpipat, S., Rueangruklikhit, T., Piyatiratitivorakul, S. (2008) Evaluations of lactic acid bacteria as probiotics for juvenile seabass Lates calcarifer. Aquac Res. 39: 134-143.
Sharifuzzaman, S.M., Austin, B. (2009) Influence of probiotic feeding duration on disease resistance and immune parameters in rainbow trout. Fish Shellfish Immun. 27: 440-445.
Soltani, M., Kalbassi, M.R. (2001) Protection of  Persian sturgeon (Acipenser persicus) fingerling against Aeromonas hydrophila septicemia using three different antigens. Bull Eur Assn Fish P. 21: 235- 240.
Soltani, M., Pourkazemi, M., Ahmadi, M.R., Taherimirghead, A., Merrifield, D.L., Masouleh, A.S. (2013) Genetic diversity of lactic acid bacteria in the intestine of Persian sturgeon fingerlings. J Appl Ichthyol. 29: 494-498.
Son,V.M., Chang, C., Wu, M., Guu,  Y., Chiu, C., Cheng, W. (2009) Dietary administration of the probiotic, Lactobacillus plantarum, enhanced the growth, innate immune responses, and disease resistance of the grouper Epinephelus coioides. Fish Shellfish Immun. 26: 691-698.
Vazquez, J.A., Gonza´lez, M.P., Muradoet, M.A. (2005) Effects of lactic acid bacteria cultures on pathogenic microbiota from fish. Aquaculture. 245: 149- 161.
Vuilaume, A., Brun, R., Chene, P., Sochoni, E., Lesel, E. (1986) First isolation of Yersinia ruckeri from Sturgeon (Acipenser baeri). Branot. In South West of France, Bult. Eur. Ass. Fish Pathology. Bull Eur Assn Fish P. 7: 18-21.
Yanbo, W., Zirong, X. (2006) Effect of probiotics for common carp (Cyprinus carpio) based on growth performance and digestive enzyme activities. Anim Feed Sci Tech. 127: 283-292.
Yang, W., Li, A. (2009) Isolation and characterization of Streptococcus dysgalactiae from diseased Acipenser schrenckii. Aquaculture. 294: 14-17.