Molecular detection of Haemoproteus in homing pigeons (Colombia livia domestica) in Mazandaran Province


1 1Department of Cellular and Molecular, Babol Branch, Islamic Azad University, Babol, Iran

2 Department of Veterinary Parasitology, Babol Branch, Islamic Azad University, Babol, Iran.

3 Department of Veterinary Parasitology,Tehran Veterinary Faculty,Tehran University,Tehran Iran

4 2-Department of Veterinary Parasitology, Tehran University, Tehran, Iran


Background: Haemoproteus is a parasitic protozoa that over 120 species of it has been reported from wild aquatic birds, sparrows and other birds orders. So far, no study has been performed to determine the species of this protozoa in the north of Iran.
Objectives: The aim of this study was to investigate the molecular and structural properties of Heamoproteus protozoa in the blood of infected pigeons in Mazandaran province.
Methods: In the present study molecular investigation of Haemoproteus infection was carried out in domestic pigeons of Mazandaran province. For this purpose, samples obtained randomly from 150 pigeons in different regions of Mazandaran. At first, blood samples were stained with Gimsa stain and examined for presence of Haemoproteus gametocytes. Then, positive samples were used for PCR by Cytochrome b genes..
Results: Obtained results after morphological survey showed that 17 samples were positive indicating infection rate of 11.33%. Molecular investigation and analysis of PCR products showed that all of the samples belonged to Haemoproteus columbae species.
Conclusion: So, being precisely familiar with this kind of protozoan and its species can limit many mistakes and be helpful in differential diagnosis of different species. This study has revealed that the most common species of Haemoproteus in Mazanadran province is Haemoproteus columbae.


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