Comparative evaluation of the effects of chitosan and atorvastatin on serum lipid profile changes in dog

Document Type : Hematology and Clinical Pathology

Authors

1 1Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Graduated of Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

BACKGROUND: Many drugs such as atorvastatin are known effective in reduction of serum lipids in dogs, but with a literature review, we did not find report on the field of the effect of chitosan on serum lipid in dogs. OBJECTIVES: The purpose of the present survey was comparative evaluation of the effects of chitosan and atorvastatin on serum lipid profile changes and the influence of time on treatment process in dogs. METHODS: For the management of cholesterol powder induced hyperlipidemia, twenty healthy dogs were randomly divided into four equal groups. Group A (control) included of five dogs that were fed with cholesterol powder (4 gr/kg for 10 days). Group B was similar to group A, but in addition, atorvastatin (5 mg/kg) was administered for 45 days after induced hyperlipidemia. Group C was similar to group B, but chitosan (3 gr/dog) was administered instead of atorvastatin. Group D was a combination of groups B and C, which the combination of atorvastatin and chitosan were fed to dogs with the same dose of previous groups. Blood samples were collected four times on days 0, 10, 40 and 55 after challenge, then serum triglycerides, total cholesterol, HDL-C and LDL-C levels were measured using standard commercial kits. RESULTS: Groups of atorvastatin and chitosan (B and C) and group D were more effective in lowering serum triglyceride, total cholesterol and LDL-C and increase of HDL-C, compared with group A (p<0.05). The greatest decrease was related to group D for triglyceride (105.60±17.49), total cholesterol (119.80±11.39) and LDL-C (36.40±7.57). The greatest increase was seen in group D for HDL-C (36.40±7.57) also. In comparison between two drugs and their effects on lipid profiles, atorvastatin showed a significant difference than chitosan (p<0.05). A combination of two drugs, was more effective compared with single administration of the drugs (p<0.05). CONCLUSIONS: The present survey showed that although both drugs have hypolipidemic activity in dogs, but the effect of chitosan was lower than atorvastatin, so it is not recommended to use chitosan only. Further experimentation needs to elucidate the possible mechanism of the drugs.

Keywords

References

Bauer, J.E. (2004) Lipoprotein-mediated transport of dietary and synthesized lipids and lipid abnormalities of dogs and cats. J Am Vet Med Assoc. 224: 668-675.##
Bokura, H., Kobayashi, S. (2003) Chitosan decreases total cholesterol in women: a randomized, double-blind, placebo-controlled trial. Eur J Clin Nutr. 57: 721-725.##
Briand, F., Magot, T., Krempf, M., Nguyen, P., Ouguerram, K. (2006a) Effects of atorvastatin on high-density lipoprotein apolipoprotein A-I metabolism in dogs. Eur J Clin Invest. 36: 224-230.##
Briand, F., Serisier, S., Krempf, M., Siliart, B., Magot, T., Ouguerram, K., Nguyen, P. (2006b) Atorvastatin increases intestinal cholesterol absorption in dogs. J Nutr. 136: 2034-2036.##
Chen, D., Zhou, D., Qian, J., Chen, F., Guan, L., Dong, L., Ge, J. (2012) Atorvastatin prevents dehydromonocrotaline-induced pulmonary hypertension in beagles. Exp Lung Res. 38: 333-343. ##
Chiang, M.T., Hsien, T.Y., Hsing, C.C. (2000) Effect Of Dietary chitosan with different viscosity on plasma lipid and peroxidation in rats fed on a diet enriched with cholesterol. Biosci Biotechnol Biochem. 64: 965-971.##
Choi, C.R., Kim, E.K., Kim, Y.S., Je, J.Y., An, S.H., Lee, J.D., Wang, J.H., Ki, S.S., Jeon, B.T., Moon, S.H., Park, P.J. (2012) Chitooligosaccharides decreases plasma lipid levels in healthy men. Int J Food Sci Nutr. 63: 103-106.##
Cunningham, S.M., Rush, J.E., Freeman, L.M. (2013) Short-term effects of atorvastatin in normal dogs and dogs with congestive heart failure due to myxomatous mitral valve disease. J Vet Intern Med. 27: 985-989. ##
Davis, H.R., Pula, K.K., Alton, K.B., Burrier, R.E., Watkins, R.W. (2001) The synergistic hypocholesterolemic activity of the potent cholesterol absorption inhibitor, ezetimibe, in combination with 3-hydroxy-3-methylglutaryl coenzyme a reductase inhibitors in dogs. Metab. 50: 1234-1241. ##
Dixon, R.M., Reid, S.W., Mooney, C.T. (1999) Epidemiological, clinical, haematological and biochemical characteristics of canine hypothyroidism. Vet Rec. 145: 481-487.##
Ford, R.B. (1996) Clinical management of lipemic patients. Compend Contin Educ Vet. 18: 1053-1060.
Fukada, Y., Kimura, K., Ayaki, Y. (1991) Effect of chitosan feeding on intestinal bile acid metabolism in rats. Lipids. 26: 395-399.##
Gades, M.D., Stern, J.S. (2003) Chitosan supplementation and fecal fat excretion in men. Obes Res. 11: 683-688.##
Gallaher, D.D., Gallaher, C.M., ahrat, J., Carr, P., Hollingshead, C.H., Heslink, R. (2002) A Glucomannan and Chitosan Fiber Supplement Decreases Plasma Cholesterol and Increases Cholesterol Excretion in Overweight Normocholesterolemic Humans. Nutr. 21: 428-433.##
Grondin, F., LeHoux, J.G. (1993) Some Effect of Chitosan on Liver Function in the Rat. Endocrin. 132: 1078-1084.##
Hur, S.J., Kim, D.H., Chun, S.C., Lee, S.K., Keum, Y.S. (2013) Effects of biopolymer encapsulation on trans fatty acid digestibility in an in vitro human digestion system. Food Funct. 4: 1827-1834.##
Johnson, M.C. (2005) Hyperlipidemia disorders in dogs. Compend Contin Educ Vet. 27: 361-364. ##
Kashyap, M.L., McGovern, M.E., Berra, K., Guyton, J.R., Kwiterovich, P.O., Harper, W.L., Toth, P.D., Favrot, L.K., Kerzner, B., Nash, S.D., Bays, H.E., Simmons, P.D. (2002) Long-term safety and efficacy of a once-daily niacin/lovastatin formulation for patients with dyslipidemia. Am J Cardiol. 89: 672-678.##
Khoushab, F., Yamabhai, M. (2010) Chitin Research Revisited. Mar Drugs. 8: 1988-2012. ##
Kubo, M. (2001) Clinical characteristics of hypertriglyceridemia-induced pancreatitis. Jpn Clin Med. 59: 698-701.##
Lee, H.W., Park, Y.S., Choi, J.W., Yi, S.Y., Shin, W.S. (2003) Antidiabetic Effects of Chitosan Oligosaccharides In Neonatal Streptozotocin- Induced Noninsulin-Dependent Diabetes Mellitus In Rats. Biol Pharm Bull. 26: 1100-1103.##
Maldonado, E.N., Romero, J.R., Ochoa, B., Aveldano, M.I. (2001) Lipid and fatty acid composition of canine lipoproteins. Comp Biochem Physiol Biochem Mol Biol. 128: 719-729.##
Mazaki-Tovi, M., Abood, S.K., Schenck, P.A. (2014) Fish oil supplementation increases concentration of adiponectin in healthy dogs. J Small Anim Pract. 55: 247-253.##
Ormrod, D.J., Holmes, C.C., Miller, T.E. (1998) Dietry Chitosan Iinhibits Hypercholesterolemia and Atherogenesis in the Apolipoprptein E- deficient Mouse Model of Atherosclerosis. Atherosclerosis. 138: 329-334.##
Prueksaritanont, T., Zhao, J.J., Ma, B., Roadcap, B.A., Tang, C., Qiu, Y., Liu, L., Lin, J.H., Pearson, P.G., Baillie, T.A. (2002) Mechanistic studies on metabolic interactions between gemfibrozil and statins. J Pharmacol Exp Ther. 301: 1042-1051.##
Puiggros, C., Chacon, P., Armadans, L.I., Clapes, J., Planas, M. (2002) Effects of oleic-rich and omega-3-rich diets on serum lipid pattern and lipid oxidation in mildly hypercholesterolemic patients. Clin Nutr. 21: 79-87.##
Sakamoto, S., Kashiki, M., Imai, N., Liang, C.S. (1991) Effects of short-term, diet-induced hypercholesterolemia on systemic hemodynamics, myocardial blood flow, and infarct size in awake dogs with acute myocardial infarction. Circulation. 84: 378-386.##
Singla, A.K., Chawla, M. (2001) Chitosan: some pharmaceutical and biological aspects-an update. J Pharm Pharmacol. 53: 1047-1067.##
Stalenhoef, A.F., de Graaf, J., Wittekoek, M.E., Bredie, S.J., Demacker, P.N., Kastelein, J.J. (2000) The effect of concentrated n-3 fatty acids versus gemfibrozil on plasma lipoproteins, low density lipoprotein heterogeneity and oxidizability in patients with hypertriglyceridemia. Atherosclerosis. 153: 129-138.##
Tan, S., Gao, B., Tao, Y., Guo, J., Su, Z.Q. (2014) Antiobese effects of capsaicin-chitosan microsphere (CCMS) in obese rats induced by high fat diet. J Agri Food Chem. 62: 1866-1874.##
Tuccori, M., Montagnani, S., Mantarro, S., Capogrosso-Sansone, A., Ruggiero, E., Saporiti, A., Antonioli, L., Fornai, M., Blandizzi, C. (2014) Neuropsychiatric adverse events associated with statins: epidemiology, pathophysiology, prevention and management. CNS Drugs. 28: 249-272. ##
Walsh, K.M., Rothwell, C.E. (1999) Hepatic effects in beagle dogs administered atorvastatin, a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor, for 2 years. Toxicol Pathol. 27: 395-401.##
Xenoulis, P.G., Steiner, J.M. (2010) Lipid metabolism and hyperlipidemia in dogs. Vet J. 183: 12-21.##
Xenoulis, P.G., Suchodolski, J.S., Levinski, M.D., Steiner, J.M. (2008) Serum liver enzyme activities in healthy Miniature Schnauzers with and without hypertriglyceridemia. J Am Vet Med Assoc. 232: 63-67.##
Yen, Y.Y., Liu, L. (2001) Cholesterol lowering effect of garlic extracts and organo-sulpur compounds: Human and animal studies. J Nutr. 131: 989-993.##
Ylitalo, R., Lehtinen, S., Wuolijoki, E., Ylitalo, P., Lehtimaki, T. (2002) Cholesterol-lowering properties and safety of chitosan. Drug Res. 52: 1-7.##
Zuhair, M.H., Luma, T.A., Qutuba, Gh. (2013) Effect of Atorvastatin And Garlic on Lipid Profile in Hyperlipidemic Patients. Diyala J Med. 63: 63-68.##
Journal of Veterinary Research
Volume 73, Issue 1
March 2018
Pages 101-109

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