Effects of triploidization by heat shock treatment on growth performance and amino acids profiles in rainbow trout (Oncorhynchus mykiss)

Document Type : Aquatic Animal Health Management

Authors

1 1Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Sciences and Technology, Khorramshahr, Iran

2 2Department of Natural Resources, Isfahan University of Technology, Isfahan, Iran

3 Department of Fisheries, Faculty of Natural Resources, University of Kurdistan, Sanandaj, Iran

Abstract

BACKGROUND: The induction of triploidy is an effective strategy for the production of sterile fish for aquaculture and has usually been reported to be accompanied by modifications in physiological characteristics. OBJECTIVES: The aim of this study was to assess the effects of triploidy induction on growth performance and amino acid profiles in rainbow trout. METHODS: Eight female (total weight 1600 ± 246 g) and six male (total weight 1393 ± 186 g) four-year-old healthy rainbow trout were used in this study. Triploidy was inducted through the application of heat shock of 28 ºC for 10 min to eggs 10 min post fertilization in an aquarium equipped with a heater. During the 38 days of rearing period, the fish were fed a rainbow trout commercial diet (BioMar, France) 12 times a day at the rate of 7% of their body weight. Fish ploidy level was determined by erythrocyte size measurement. RESULTS: Based on red blood cell analysis, the overall triploidization success level was 87.1%. Growth performance was significantly higher in diploids as compared to triploids (p<0.05). The levels of non essential amino acids increased and the levels of essential amino acids decreased as an effect of triploidy induction. CONCLUSIONS: Triploidy induction in rainbow trout affects growth performance and amino acid profiles in rainbow trout.

Keywords

References

Barton, B. A. (2002) Stress in fish: A diversity of responses with particular reference to change in circulating corticosteroids. Integer Comp Biol. 42: 217-225.##
Benfey, T. J. (1999) The physiology and behavior of triploid fishes. Rev in Fish Sci. 7: 39-67.##
Benfey, T. G., Sutterlin A.M. (1984) The hematology of the triploid landlocked Atlantic salmon, Salmo salar.  J  Fish Biol. 24: 333-338.##
Buchtova, H. Svobodova, Z., Flaj, M., Vorlova, L. (2003) Analysis of growth, weight and relevant indices of diploid and triploid population of tench, Tinca tinca. Aquac Res. 34: 719-726.##
Buchtova,  H., Smutna, M.,  Vorlova, L., Svobodova, Z., Flajshans, M. ( 2005)  Amino Acid Composition of Muscle Proteins of Diploid and Triploid Tench,  Tinca tinca. Acta Vet Brno. 74: 329-337.##
Dunham, R. A. (2001) Aquaculture and Fisheries Biotechnology, Genetic Approaches. CABI Publishing. p. 22-53.##
Fu, C.,  Cui, Y.,  Hung, S. S. O.,  Zhu, Z. (2000) Whole-body amino acid pattern of F4 human growth hormone genetransgenic red common carp, Cyprinus carpio fed diets with different protein levels. Aquaculture. 189: 287-292.##
Hochachka, P.W., Mommsen, T.P. (1995) Metabolic Biochemistry (Biochemistry and molecular biology of fishes). (1st ed.) Elsevier, Amsterdam. The Nederlands. 515 p.##
Ihssen, P. E., McKay, L. R., McMillan, I., Phillips, R. B. (1990) Ploidy manipulation and gynogenesis in fishes: cytogenetic and fisheries applications. Trans Am Fish Soc. 119: 698-717.##
Kaushik, S. J., Luquet, P. (1977) Study of free amino acids in rainbow trout in relation to salinity changes: I. Blood free amino acids during starvation. Ann  Hydrobiol. 8: 135-144.##
Mohanty, S. N., Kaushik, S. J. (1991) Whole body amino acid composition of Indian major carp and its significance. Aquat Living Resour. 4: 61-64.##
Omoto, N., Maebayashi, M., Adachi, S., Arai, K., Yamauchi, K. (2005) Sex ratios of triploids and gynogenetic diploids induced in the hybrid sturgeon, the bester (Huso huso × Acipenser ruthenus). Aquaculture. 245: 39-47.##
Pandian, T. J., Koteeswaran, R. (1998) Ploidy induction and sex control in fish. Hydrobiologia. 384: 167-243.##
Piferrer, F., Beaumont, A., Falguière, J. C., Flajšhans, M., Haffray, P., Colombo, L. (2009) Polyploid fish and shellfish: production, biology and applications to aquaculture for performance improvement and genetic containment. Aquaculture. 293: 125-156.##
Quilete, E., Chevassus, B., Krieg, F. (1987) Characterization of auto- and allotriploid salmonids for rearing in seawater cages. Selection, Hybridization, and Genetic Engineering in Aquaculture, vol. 3.  In: Tiews, K. (ed.). Heenemann Verlags, Berlin, Germany. p. 239-252.##
Sezaki, K., Watabe, S.,  Hashimoto, K. (1983) Comparison of chemical composition between diploids and triploids of ‘‘ginbuna’’ Carassius auratus. Bull Japan Soci  Sci Fish. 49: 97-101.##
Smith, D. S., Benfey, T. J. (2001) The reproductive physiology of three age classes of adult female diploid and triploid brook trout, Salmo trutta. Fish Physiol Biochem. 25: 319-333.##
Smutna, M., Vorlova, L., Svobodova, Z. (2002) Pathobiochemistry of ammonia in the internal environment of fish. Acta Vet Brno.71: 169-181.##
Soosean, C., Marimuthu, K., Sudhakaran, S., Xavier, R. (2010) Effects of mangosteen (Garcinia mangostana.) extracts as a feed additive on growth and haematological parameters of African catfish. Clarias gariepinus fingerlings. Eur Rev for Med Pharm Sci. 14: 605-611.##
Strunjak, I., Rakovak, R.A., Topic, N. (2003) Micronucleus occurrence in diploid and triploid rainbow trout, Oncorhynchus mykiss. Vet Med. 48: 215-219.##
Tiwary, B. K., Kirubagaran, R., Ray, A. K. (2004) The biology of triploid fish. Rev Fish Biol Fish. 14: 391-402.##
Walton, M. J., Wilson, R. P. (1986) Postprandial changes in plasma and liver free amino acids of rainbow trout fed complete diets containing casein. Aquaculture. 51: 105-115.##
Wilson, R.P. (1989) Amino acids and proteins. In: Fish Nutrition.  Halver, J.E. (ed.). Academic Press, San Diego, USA. p. 111-151.##
Yamamoto, T., Unuma, T., Akiyama, T. (2000) The influence of dietary protein and fat levels on tissue free amino acid levels of fingerling rainbow trout, Oncorhynchus mykiss. Aquaculture. 182: 353-372.##
Yokoyama, M.,  Nakazoe, J. (1991)  Effects of dietary protein levels on free amino acid and glutathione contents in the tissues of rainbow trout. Comp Biochem Physiol A. 99: 203-206.##
Yokoyama, M., Udagawa, M., Nakazoe, J. (1994) Influence of dietary protein levels on hepatic cysteine dioxygenase activity in rainbow trout. Fish Sci. 60: 229-233.##
Journal of Veterinary Research
Volume 73, Issue 2
June 2018
Pages 173-180

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