Assessing the Serum Metabolic Biomarkers of Darehshori Horses and Their Relationships With Each Other

Document Type : Large Animal Health Management


1 Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

2 D.V.M, Private Sector, Iran


BACKGROUND: Different breeds of horses have distinct and
specific metabolic profiles which reflect the health status of horses. OBJECTIVES:
The present study was performed to determine the circulating metabolic
biomarkers and their correlations in Darehshori horses. METHODS: Blood
samples were taken from 56 Darehshori horses (5 to 7 years old). They were
divided into 2 groups containing 26 non-pregnant and non-lactating mares and 30
stallions. Sera were separated and assayed for glucose, insulin,
beta-hydroxybutiric acid, non-esterified fatty acid, cholesterol, triglyceride
and high, low and very low density lipoproteins. RESULTS: Serum concentration
of beta-hydroxybutiric acid in stallions was significantly higher than in
mares. The remaining metabolic biomarkers showed no significant differences
between the two studied groups. There were negative and non- significant
correlations between insulin and glucose in both sexes. Insulin was negatively
correlated with cholesterol, triglyceride and high, low and very low density
lipoproteins in studied groups. The negative relationships were seen among
glucose and cholesterol, triglyceride and high, low and very low density
lipoproteins in mares and stallions. In both groups, non-esterified fatty acid
and beta-hydroxybutiric acid were negatively correlated with serum lipid
profile. CONCLUSIONS: The results of the present study provided a
general pattern for normal values of metabolic profile biomarkers in
non-pregnant mares and stallions of Darehshori horses. These values also help
veterinarians to diagnose metabolic abnormalities of this breed. However,
season, age, management, nutritional and different physiological states may
alter the values of these biomarkers and in interpreting the changes, these
conditions should be considered


Abell, L.L., Levy, B.B., Brodie, B.B., Kendall, F.E. (1952) A simplified method forthe estimation of total cholesterol in serumand determination of its specificity. J Biol Chem. 195: 357-366.##
Afifi, A., Kraft, W., Arif, H. (1979) Values of rT3, T4, total T3 and cholesterol of some farm animals in Egypt. Indian Vet J. 56: 16-18.##
Asadi, F., Mohri, M., Adibmoradi, M. Pourkabir, M. (2006) Serum lipid and lipoprotein parameters of Turkman horses. Vet Clin Pathol. 35: 332-334.##
Boden, G., Laakso, M. (2004) Lipids and glucose in type 2 diabetes: what is the cause and effect? Diabetes Care. 27: 2253-2259.##
Buff, P.R., Morrison, C.D., Ganjam, V.K., Keisler, D.H. (2005): Effects of shorttermfeed deprivation and melatonin implants on circadian patternsof leptin in the horse. J Anim Sci. 83: 1023-1032.##
Burtis, C.A., Ashwood, E.R. (1994) Tietz Textbook of Clinical Chemistry. (2nd ed.). W.B. Saunders Com, Philadelphia, USA.##
Carr, M.C., Brunzell, J.D. (2004) Abdominal obesity and dyslipidemiain the metabolic syndrome: importance of type 2 diabetes and familialcombined hyperlipidemia in coronary artery disease risk. J Clin Endocrinol Metab. 89: 2601-2607.##
Davis, A.J., Evans, D.L. (2000) Blood lactate responses tosubmaximal field exercise tests in Thoroughbred horses.Vet J. 159: 252-258.##
DeKoster, J.D., Opsomer, G. (2013) Insulin resistance in dairy cows. Vet Clin N Am Food Anim Pract. 29: 299-322.##
Emery, R.S., Liesman, J.S., Herdt, T.H. (1992) Metabolism of long chain fatty acids by ruminant liver. J Nutr. 122: 832-837.##
Fiore, E., Gianesella, M., Arfuso, F., Giudice, E., Piccione, G., Lora, M., Stefani, A., Morgante, M. (2014) Glucose infusion response on some metabolic parameters in dairy cows during transition period. Arch Tierz. 57:1-9.##
Frank, N. (2011) Equine metabolic syndrome. Vet Clin North Am Equine Pract. 27: 73-92. ##
Frank, N., Elliott, S.B., Brandt, L.E., Keisler, D.H. (2006) Physical characteristics, blood hormone concentrations, and plasma lipid concentrations in obese horses with insulin resistance. J Am Vet Med Assoc. 228:1383-1390.##
Frank, N., Sojka, J.E., Latour, M.A. (2003) Effects of hypothyroidismand withholding of feed on plasma lipid concentrations, concentrationand composition of very-low-density lipoprotein, and plasmalipase activity in horses. Am J Vet Res. 64: 823-828.##
Friedewald, W.T., Levy, R.I., Fredrickson, D.S. (1972) Estimation of the concentrationof low density lipoprotein cholesterol without the use of the preparative ultracentrifuge. Clin Chem. 18: 499-502.##
Gomide, L.M.W., Martins, C.B., Orozco, C.A.G., Sampaio, R.C.L., Belli, T., Baldissera, V., Lacerdaneto, J.C. (2006) Blood lactate concentrations of horses competing in the resistance phase of 3-day combined training event. Cienc Rural. 36: 509-513.##
Grummer, R.R., Mashek, D.G., Hayirli, A. (2004) Dry matter intake and energy balance in the transition period. Vet Clin N Am Food Anim Pract. 20: 447-470.##
Hasso, S.A., Al-Hadithy, H.A., Hameed, R.M. (2012) Serum glucose concentration and lipid profile in racing horses. Iraqi J Vet Sci. 26: 1-3.##
Henneke, D.R., Potter, G.D., Kreider, J.L., Yeates, B.F. (1983) Relationship between condition score, physical measurements and body fat percentage in mares. Equine Vet J. 15: 371-372.##
Hoffman, R.M., Boston, R.C., Stefanovski, D., Kronfeld, D.S., Harris,P. A. (2003) Obesity and diet affect glucose dynamics and insulin sensitivity in Thoroughbred geldings. J Anim Sci. 81: 2333-2342.##
Johnson, P.J. (2002) The equine metabolic syndrome peripheral Cushing’ssyndrome. Vet Clin North Am Equine Pract. 18: 271-293.##
Kaneko, J.J. (1989) Clinical Biochemistry of Domestic Animals. Academic Press. NewYork. U.S.A. p. 83-115.##
Kronfeld, D.S., Treiber, K.H., Geor, R.J. (2005) Comparison of nonspecific indications and quantitative methods for the assessment of insulin resistance in horses and ponies. J Am Vet Med Assoc. 226: 712-719.
Kronfeld, D.S. (2003) Equine syndrome X, the metabolic syndrome, and equine grain-associated disorders: nomenclature and dietetics. J Equine Vet Sci. 23: 567-569.##
Lacerda, L., Campos, R., Sperb, M., Soares, E., Barbosa, P., Godinho, E., Ferreira, R., Santos, V., González, F.D. (2006) Hematologic and Biochemical parameters in three high performance horse breeds from southern brazil. Arch Vet Sci. 11: 40-44. ##
McGowan, M.W., Artiss, J.D., Strandbergh, D.R., Zak, B. (1983) A peroxidasecoupled method for the colorimetric determinationof serum triglycerides. Clin Chem. 29: 538-542.##
Nadeau, J.A., Frank, N., Valipe, S.R., Elliot, S.B. (2006) Blood lipid, glucose, and insulin concentrations in Morgan horses and Thoroughbreds. J Equine Vet Sci. 26: 401-405.##
Nazifi, S., Saeb, M., Rategh, S., Khojandi, A. (2005) Serum lipids and lipoproteins in clinically healthy Caspian miniature horses. Vet Arhiv. 75: 175-182.##
Nazifi, S., Saeb, M., Abedi, M. (2003) Serum lipid profiles and their correlation with thyroid hormones in clinically healthy Turkoman horses. Comp Clin Path. 12: 49-52.##
Powell, D.M., Reedy, S.E., Sessions, D.R., Fitzgerald, B.P. (2002) Effect of short term exercise training on insulin sensitivity in obese and lean mares. Equine Vet J. 34: 81-84.##
Pullen, D.L., Palmquist, D.L., Emery, R.S. (1989) Effect on days of lactation and methioninehydroxy analog on incorporation of plasma fatty acids into plasma triglycerides. J Dairy Sci. 72: 49-58.##
Reaven, G.M. (2005) Compensatory hyperinsulinemia and the development of an atherogenic lipoprotein profile: the price paid tomaintain glucose homeostasis in insulin-resistant individuals. Endocrinol Metab Clin North Am. 34: 49-62.##
Robinson, N.E. (1997) Current Therapy in Equine Medicine. W.B. Saunders Co. Philadelphia. U.S.A. p. 698-704.##
Van Weyenberg, S., Hesta, M., Buyse, J., Janssens, G.P. (2008) The effect of weight loss by energy restriction on metabolic profile and glucose tolerance in ponies. J Anim Physiol Anim Nutr. (Berl). 92: 538-545.##
Watson, T.D., Burns, L., Freeman, D. J., Packard, C.J., Shepherd, J. (1993) High density lipoprotein metabolism in the horse (Equus caballus). Comp Biochem Physiol. 104: 45-53.##