Effect of Oral Calcium Chloride Supplementation on Serum Calcium, Phosphorus and Magnesium of Holstein Dairy Cows in Transition Period

Document Type : Large Animal Health Management


1 1Graduate From the Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 2Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran 3Department of Animal Health Management, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran

3 2Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

4 Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran


BACKGROUND: Loss of calcium around calving can lead to diseases of transition period and reduce animal economic life. Prevention of milk fever and subclinical hypocalcemia is crucial and important in this period. Repeated doses of oral calcium chloride at calving is a method to prevent hypocalcemia and associated complications. ObjectiveS: The objective of this study was to evaluate the influence of oral calcium chloride at calving on serum calcium, phosphorus and magnesium in transitional period of Holstein dairy cows fed with anionic and cationic diets. Methods: Forty-two Holstein dairy cows were randomly divided in 3 groups. Group 1 (n = 14), fed diet with negative DCAD without calcium chloride supplementation. Group 2 (n = 14), fed diet with negative DCAD and supplemented with calcium chloride at calving and 12 h later. Group 3 (n = 14), fed diets with positive DCAD and supplemented with calcium chloride at calving and 12 h later. Blood samples were collected at calving and 6 h and 12 h and 1d, 2 d, 7 d, 14 d, 21 and 28 d after calving. Serum concentrations of Ca, P and Mg were measured by conventional methods. Results: The pattern of changes in serum levels of calcium and magnesium in different groups in different time periods (time × treatment interaction) were different (p<0.0001). Changes in serum phosphorus levels in different time periods were statistically significant (p<0.0001), but its mean was not affected by the treatment groups (p=0.7164).                    ConclusionS: In addition to anionic diets, supplemental calcium chloride should be used to prevent subclinical hypocalcemia in high-producing dairy cows.


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