Comparison of Neospora caninum Infected Tissues in Aborted Fetal Bovine by PCR

Document Type : Obstetrics & Theriogenology


1 1Department of Obstetrics and Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Pathology, Qazvin University of Medical Sciences, Qazvin, Iran

3 Graduated From the Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran


BACKGROUND: Neospora caninum is a protozoan
intracellular parasite which is considered as one of the main factors for
recurrent abortions of dairy cattle in various countries such as Iran. This
parasite leads to negative economic impacts such as decline in reproduction,
reduced amount of milk, and long calving intervals. OBJECTIVES: There
have been numerous tests to determine the cause of abortion. PCR test is
considered as a suitable method to specify Neospora caninum DNA and it
can determine the DNA in tissue samples and body fluids of the aborted fetus.
This study aims to use PCR to evaluate parasites in the tissues of aborted
fetuses so as to detect the best tissue for determining the parasite. METHODS:
In this study, 82 aborted fetuses in the first six months of 2015 were studied.
The tissues were selected from brain, liver, lungs, spleen, kidneys and rennet
fluids. The NP21plus primer was used to detect the presence of Neospora
in samples. After conducting the PCR Test, samples with 340bp band
in Gel electrophoresis were considered as positive. Statistical data from the
survey of Neospora caninum’s presence in selected tissues were evaluated
by SAS (version 9.2) software. RESULTS: Contamination with this parasite
was found in 34 brain samples (41.5%) of aborted fetuses. In 2 (2.4%) and 4
(4.9%) of the aborted fetuses, parasite DNA was found in lung and liver tissues
along with brain tissues, respectively. CONCLUSIONS: Due to significant
difference of infection of brain tissues in comparison to other tissues, our
study considers brain tissue as the most appropriate sample for detecting Neospora
infection in aborted fetuses in PCR method


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