The Role of Ventral Hippocampal (CA3) 5-HT4 Receptor in Rats Emotional Memory in Elevated Plus-Maze Task

Document Type : Basic Sciences

Authors

1 Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran

2 2Iranian National Center for Addiction Studies, Tehran University of Medical Sciences, Tehran, Iran

3 3Cognitive and Neuroscience Research Center (CNRC), Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran

Abstract

BACKGROUND: Many studies have demonstrated the important role of hippocampal serotonergic receptors in the emotional memory formation. Therefore, it is necessary to investigate the possible role of hippocampal serotonergic receptors in emotional memory formation in several animal model tasks. OBJECTIVES: The aim of this study was to investigate the possible role of ventral hippocampal (CA3) 5-HT4 receptors in emotional memory formation of rats in elevated plus-maze task. METHODS: 102 male Wistar rats were divided to 16 groups  (n=7), randomly. Two guide cannulae were implanted bilaterally, into the left and right hippocampi, using stereotaxic apparatus. At the test time and following the injections of saline, 5-HT4 receptor’s agonist and/or antagonist, the elevated plus-maze was used for evaluation of the emotional memory. RESULTS: A pre-test intra-CA3 injection of competitive agonist (RS67333; 1.2 µg/rat) and competitive antagonist (RS23597-190; 1.2 µg/rat) of 5-HT4 receptor, increased OAT% and OAE% by themselves while only the RS23597-190 decreased the closed arm entries, indicating that these drugs impaired the memory formation. Also, a pre-retest intra-CA3 injection of RS67333 and RS23597-190 (1.2 µg/rat) increased OAT% and OAE% by themselves, indicating that these drugs prevented the recall of the memories that formed on first day. CONCLUSIONS: The results revealed that the hippocampal 5-HT4 serotonergic receptor have an important role in memory formation and memory recall, at the time of emotional arousal and stressful situations.

Keywords

References

Ahmadi, H., Nasehi, M., Rostami, P., Zarrindast, M.R. (2013) Involvement of the nucleus accumbens shell dopaminergic system in prelimbic NMDA-induced anxiolytic-like behaviors. Neuropharmacology. 71: 112-123.##
Bertoglio, L.J., Joca, S.R.L., Guimarães, F.S. (2006) Further evidence that anxiety and memory are regionally dissociated within the hippocampus. Behav Brain Res.175: 183-188.##
Bockaert, J., Claeysen, S., Compan, V., Dumuis, A. (2004) 5-HT4 receptors. Curr Drug Targets CNS Neurol Disord. 3: 39-51.##
Buhot, M.C., Wolff, M., Segu, L. (2004) Serotonin. In: From messengers to molecules: Memories are made of these. Riedel, G., Platt, B. (eds.). (1st ed.) Springer Science & Business Media. New York, NY  USA.  p. 125-142.##
Charnay, Y., Léger, L. (2010) Brain serotonergic circuitries. Dialogues Clin Neurosci. 12: 471-487.##
Chegini, H.R., Nasehi, M., Zarrindast, M.R. (2014) Differential role of the basolateral amygdala 5-HT3 and 5-HT4 serotonin receptors upon ACPA-induced anxiolytic-like behaviors and emotional memory deficit in mice. Behav Brain Res. 261: 114-126.##
Christianson, S.A. (1992) Remembering emotional events: Potential mechanisms. In: The Handbook of Emotion and Memory: Research and Theory. Christianson, S.A.(ed.). (1st ed.) Lawrence Erlbaum Associates. Hillsdale, NJ, USA. p. 307-340.##
Galeotti, N., Ghelardini, C., Bartolini, A. (1998) Role of 5-HT4 receptors in the mouse passive avoidance test. J Pharmacol Exp Ther. 286: 1115-1121.##
9. Hensler, J.G. (2006) Serotonergic modulation of the limbic system. Neurosci Biobehav Rev. 30: 203-214.##
Lechin, F., van der Dijs, B., Lechin, M.E. (2002) Neurocircuitry and Neuroautonomic Disorders: Reviews and Therapeutic Strategies. (1st ed.) Karger Medical and Scientific Publishers. Basel, Switzerland. p. 3-4.##
Lezoualc’h, F., Berthouze, M. (2006) 5-HT3 and 5-HT4 receptors as targets for drug discovery for dementia. In: The Serotonin Receptors. From Molecular Pharmacology to Human Therapeutic. Roth, B.L.(ed.). (1st ed.) Humana Press Inc. Totowa, NJ, USA. p. 459-479.##
Lezoualc’h, F., Robert, S.J. (2003) The serotonin 5-HT4 receptor and the amyloid precursor protein processing. Exp Gerontol. 38: 159-166.##
Mamounas, L.A., Mullen, C.A., O’hearn, E., Molliver, M.E. (1991) Dual serotoninergic projections to forebrain in the rat: Morphologically distinct 5-HT axon terminals exhibit differential vulnerability to neurotoxic amphetamine derivatives. J Comp Neurol. 314: 558-586.##
McGaugh, J.L. (2004) Memory reconsolidation hypothesis revived but restrained: theoretical comment on Biedenkapp and Rudy (2004) Behav Neurosci. 5: 1140-1142.##
Mendez-David, I., David, D.J., Darcet, F., Wu, M.V., Kerdine-Römer, S., Gardier, A.M., Hen, R. (2014) Rapid anxiolytic effects of a 5-HT4 receptor agonist are mediated by a
neurogenesis-independent mechanism. Neuropsychopharmacology. 39: 1366-1378.##
Meneses, A. (2003) A pharmacological analysis of an associative learning task: 5-HT1 to 5-HT7 receptor subtypes function on a Pavlovian/instrumental autoshaped memory. Learn Memory. 10: 363-372.##
Meneses, A. (2007) Stimulation of 5-HT 1A, 5-HT 1B, 5-HT 2A/2C, 5-HT 3 and 5-HT 4 receptors or 5-HT uptake inhibition: short-and long-term memory. Behav Brain Res. 184: 81-90.##
Meneses, A., Liy-Salmeron, G. (2012) Serotonin and emotion, learning and memory. Rev Neurosci. 23: 543-553.##
Mengod, G., Vilaró, M.T., Cortés, R., López-Giménez, J.F., Raurich, A., Palacios, J.M. (2006) Chemical neuroanatomy of 5-HT receptor subtypes in the mammalian brain. In: The Serotonin Receptors. From Molecular Pharmacology to Human Therapeutic. Roth, B.L. (ed.). (1st ed.) Humana Press Inc. Totowa, NJ, USA. p. 319-364.##
Nasehi, M. (2014) The modulatory effect of CA1 5HT 4 receptors on memory acquisition deficit induced by harmaline. JPS. 5: 61-71.##
Nasehi, M., Kafi, F., Khakpai, F., Zarrindast, M.R. (2015) Involvement of the serotonergic system of the ventral hippocampus (CA3) on amnesia induced by ACPA in mice. Behav Brain Res. 286: 356-363.##
Naseri, M.H., Hesami-Tackallou, S., Torabi-Nami, M., Zarrindast, M.R., Nasehi, M. (2014) Involvement of the CA1 GABAA receptors in MK-801-induced anxiolytic-like effects: an isobologram analysis. Behav Pharmacol. 25: 197-205.##
Pavlides, C., Watanabe, Y., McEwen, B.S. (1993) Effects of glucocorticoids on hippocampal long-term potentiation. Hippocampus. 3: 183-192.##
Paxinos, G., Watson, C. (2009) The rat brain in stereotaxic coordinate. (1st ed.) Elsevier, academic Press. San Diego, CA, USA. p. 113.##
Richardson, M.P., Strange, B.A., Dolan, R.J. (2004) Encoding of emotional memories depends on amygdala and hippocampus and their interactions. Nat Neurosci. 7: 278-285.##
Stern, C., Carobrez, A., Bertoglio, L. (2008) Aversive learning as a mechanism for lack of repeated anxiolytic-like effect in the elevated plus-maze. Pharmacol Biochem Be. 90: 545-550.##
Stern, C., Do Monte, F., Gazarini, L., Carobrez, A., Bertoglio, L. (2010) Activity in prelimbic cortex is required for adjusting the anxiety response level during the elevated plus-maze retest. Neuroscience. 170: 214-222.##
Strange, B., Dolan, R. (2004) β-Adrenergic modulation of emotional memory-evoked human amygdala and hippocampal responses. Proc Natl Acad Sci USA. 101: 11454-11458.##
Takahashi, H., Takada, Y., Urano, T., Takada, A. (2002) 5-HT4 receptors in the hippocampus modulate rat locomotor activity. Hippocampus. 12: 304-310.##
Tsoory, M., Vouimba, R., Akirav, I., Kavushansky, A., Avital, A., Richter-Levin, G. (2007) Amygdala modulation of memory-related processes in the hippocampus: potential relevance to PTSD. Prog Brain Res. 167: 35-51.##
Xiong, H. (2008) Hippocampus and spatial memory. In: Neuroimmune Pharmacology. Ikezu, T. and Gendelman, H. (eds.). (1st ed.) Springer Science & Business Media. New York, NY, USA.  p. 55-64.##
Journal of Veterinary Research
Volume 73, Issue 3
September 2018
Pages 353-360

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