Molecular Detection of Neospora caninum in Aborted Fetuses of Cattle Farms in Arak

Document Type : Large Animal Health Management

Authors

1 Razi Vaccine and Serum Research Institute, Arak, Iran

2 Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

BACKGROUND: Neospora caninum is one of the most important pathogenic protozoan parasites causing bovine abortion around the world.
Objectives: The aim of this study was to detect the presence of Neospora caninum in the brain, cerebellum and medulla oblongata of aborted fetuses in cattle in Arak by means of molecular method.
Methods: 38 samples of brain, cerebellum and medulla oblongata from aborted fetuses in dairy cattle of Arak were tested for the presence of Neospora with nested-PCR.
Results: Survey findings indicated the presence of DNA in 26.3 % of aborted fetal brains. In the cerebellum and medulla oblongata samples no Neospora caninum  DNA was detected. There was a significant relationship between neosporosis and maternal age (number of calvings), abortion history and the presence of dogs in the herd.
Conclusions: The results showed a significant association between the infection and the number of abortions in the examined cows; As a result it seems that neosporosis could be an important factor in epidemic abortions in Arak city’s dairy farms which requires continuous monitoring and implementation of prevention programs in the dairy industry.

Keywords


 
Basso, W., Venturini, L., Venturini, M. C., Hill, D. E., Kwok, O. C. H., Shen, S. K., Dubey, J. P. (2001). First isolation of Neospora caninum from the feces of a naturally infected dog. J Parasitol, 87(3): 612-618.
Dubey, J. P., Carpenter, J. L., Speer, C. A., Topper, M. J., Uggla, A. N. D. A. (1988). Newly recognized fatal protozoan disease of dogs. JAVMA, 192(9): 1269-1285.
Dubey, J. P., Lindsay, D. S., Adams, D. S., Gay, J. M., Baszler, T. V., Blagburn, B. L., Thulliez, P. (1996). Serologic responses of cattle and other animals infected with Neospora caninum. Am J Vet Res, 57(3): 329-336.
Dubey, J. P., Schares, G. (2006) Diagnosis of bovine neosporosis. Vet Parasitol, 140(1): 1-34.
Dubey, J. P., Schares, G., Ortega-Mora, L. M. (2007). Epidemiology and control of neosporosis and Neospora caninum. Clin Microbiol Rev, 20(2): 323-367.
Dubey, J.P., Hartley, W.J., Lindsay, D.S., Topper, M.J. (1990). Fatal congenital Neospora caninum infection in a lamb. J Parasitol, 76:127–130.
Dubey, J.P., Lindsay, D.S. (1996). A review of Neospora caninum and neosporosis. Vet Parasitol, 67: 1–59.
Gharekhani, J., Haddadzadeh, H., Bahonar, A. (2014). Prevalence of immunoglobulin G (IgG) antibody to Neospora caninum in dairy cattle of Hamedan province, west of Iran. Vet Res Forum, 5(2): 149-152.
Gharekhani, J., Heidari, H. (2014). Serology based comprehensive study of Neospora infection in domestic animals in Hamedan province, Iran. J Adv Vet Anim Re, 1(3): 119-124.
Gondim, L. F., McAllister, M. M., Pitt, W. C., Zemlicka, D. E. (2004). Coyotes (Canislatrans) are definitive hosts of Neospora caninum. Int J Parasitol, 34(2): 159-161.
Habibi,G. R., Hashemi-Fesharki, R., Sadrebazzaz, A., Bozorgi, S., Bordbar, N. (2005). Seminested PCR for diagnosis of Neospora caninum infection in cattle. Arch Razi Inst, 55: 55-64.
Hajikolaei, M.H., Hamidinejat, H., Ghorbanpoor, M., Goraninejad, S. (2008). Serological study of Neospora caninum infection in cattle from Ahvaz area, Iran. Int J Vet Res, 2(1): 63-66.
Heidari, H., Mohammadzadeh, A., Gharekhani, J. (2014). Seroprevalence of Neospora caninum in slaughtered native cattle in Kurdistan province, Iran. Vet Res Forum, 5(1): 69-72.
Hosseininejad, M., Hosseini, F. (2011). Seroprevalence of Neospora caninum and Toxoplasma gondii infection in dogs from west and central parts of Iran using two indirect ELISA tests and assessment of associate risk factors. Iran J Vet Res, 12(1): 46-51.
Kamali, A., Adin Seifi, H., Movassaghi, A. R., Razmi, Gh.R., Naseri, Z. (2014).  Histopathological and molecular study of Neospora caninum infection in bovine aborted fetuse. Asian Pac J Trop Biomed, 4(12): 990-994.
Macedo, C. A. B. D., Macedo, M. F. S. B. D., Cardim, S. T., Paiva, M. C. D. C., Taroda, A., Barros, L. D., Garcia, J. L. (2013). Neospora caninum: evaluation of vertical transmission in slaughtered dairy cows (Bos taurus). Rev Bras Parasitol Vet, 22(1): 13-17.
Nematollahi, A., Jaafari, R., Moghaddam,Gh.(2011). Seroprevalence of Neospora caninum infection in dairy cattle in Tabriz, Northwest Iran. Iran J Parasitol, 6(4): 95-98.
Razmi, G. (2009). Survey of dogs’ parasites in Khorasan Razavi province, Iran. Iran J Parasitol, 4(4): 48-54.
Razmi, G. R., Mohammadi, G. R., Garrosi, T., Farzaneh, N., Fallah, A. H., Maleki, M. (2006).Seroepidemiology of Neospora caninum infection in dairy cattle herds in Mashhad area. Vet Parasitol, 135(2): 187-189.
Razmi, Gh.R., Zarae, H., Nourbakhash, M.F., Naseri, Z. (2013). Estimating the rate of transplacental transmission of Neospora caninum to aborted fetuses in seropositive dams in Mashhad area. Iran J Parasitol, 7(4):253-256 25.
Salehi, N., Hadadzadeh, H., Ashrafi Helan, J., Shayan, P., Sadrebazzaz, A. (2009). Molecular and pathological study of bovine aborted fetuses and placenta from Neospora caninum infected dairy cattle. Iran J Parasitol, 4(3): 40-51.
Sattari, A., Moshiri, F., Musavi, S. Gh. (2011). The seroprevalence of Neospora caninum antibodies in dairy cattle herds in Golestan province, Iran. J Vet Microbiol, 1(22): 60-64.
Sawada, M., Park, C. H., Kondo, H., Morita, T., Shimada, A., Yamane, I., Umemura, T. (1998). Serological survey of antibody to Neospora caninum in Japanese dogs. J Vet Med Sci, 60(7): 853-854.
Wouda, W., Dijkstra, T. H., Kramer, A. M. H., Van Maanen, C., Brinkhof, J. M. A. (1999). Seroepidemiological evidence for a relationship between Neospora caninum infections in dogs and cattle. Int J Parasitol, 29(10): 1677-1682.
Youssefi, MR., Arabkhazaeli, F., Tabar Molla, H. (2009). Seroprevalence of Neospora caninum infection in rural and industrial cattle in northern Iran. Iran J Parasitol, 4(1): 15-18.