Journal of Veterinary Research

Journal of Veterinary Research

Serological Diagnosis of Infectious Haematopoietic Necrosis Disease (IHN) in Rainbow Trout (Oncorhynchus mykiss) using Indirect Fluorescent Antibody Test

Authors
Roozbeh Fallahi
Mahdi Soltani
Seyed Jalil Zorriehzahra
Farhid Hemmatzadeh
Unit of Aquatic Animal Health and Diseases, RaTi Vaccine and SerumRe search Institute, Karaj - I ran
Abstract
Objective: Serological diagnosis of infectious haematopoietic necrosis virus (IHNV) in tissues of kidney, spleen and liver of rainbow trofi(O.mykiss)fry. Design: Cross- sectional study. Animals:Ninty nine samples consisting of smears from kidney, spleen and liver were obtained from rainbow trout. Procedure: Kidney, spleen and liver from rainbow trout (O.mykiss) fry less than 3 g body weight obtained from some farmed rainbow trout of Tehran and Char mahal - va- Bakhtiari provinces. Indirect immunofluorescent antibody test (IFAT) was undertaken using both polyclonal and monoclonal antibodies to IHNV to identify the IHNV antigens in the tissue samples. Results: Only eleven samples from the region of Char mahal - va - Bakhtiari were positive for IHNV using both monoclonal and polyclonal antibodies to IHNV.Conclusion: Because of economic losses due to disease caused by IHNV in farmed rainbow trout , it is necessary to do a comprehensive screening test, virus isolation and evaluation of the pathogenicity of recovered virus.
Keywords
IFAI
IHNV
Rainbow trout