عنوان مقاله English
نویسندگان English
Sarcocyst is one of the most important protozoa belonged to apicomplexa. This parasite is prevalent
among warm blooded animals throughout the world. In the present work, Sarcocystis gigantea was
identified by amplification of 18S rRNA gene using PCR- RFLP. In this regard macroscopic cysts of
sarcocystis were collected from esophagus and intra costal muscles of sheep in Shahriar
slaughterhouse. Then genomic DNA of the parasites was extracted from specimens using phenolchloroform
method. 18S rRNA gene was amplified with specific primers. For RFLP two restricted
enzymes of MspI and MobI were used and the patterns were analyzed accordingly. According to the
resulted band, all the specimens were identified as Sarcocystis gigantea. This is the first report of
molecular identification of Sacrocystis gigantea in Iran.
کلیدواژهها English
