نویسندگان
1 گروه تحقیق و تولید واکسن‌های ویروسی طیور، موسسسه تحقیقات واکسن و سرم سازی رازی، کرج– ایران
2 گروه علوم درمانگاهی، دانشکده دامپزشکی دانشگاه تهران،تهران– ایران
3 گروه بیماریهای طیور، مرکز تشخیص اداره کل دامپزشکی استان خراسان رضوی سازمان دامپزشکی کشور، تهران– ایران
4 گروه ویروس شناسی، مرکز تشخیص اداره کل دامپزشکی استان خراسان رضوی سازمان دامپزشکی کشور، تهران– ایران
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
BACKGROUND: Infectious bronchitis is an acute, highly contagious, viral disease of poultry with worldwide distribution, and is continuously evolving through point mutation and recombination of their genome; subsequently the emergence of IBV variants complicates disease control. OBJECTIVES: To investigate genetic characterization of new IBV variants isolated from commercial chicken flocks in Iran collected between 2009 and 2010. METHODES: The partial S1 gene of the spike protein, covering a hypervariable and constant regions, was amplified and sequenced using conventional RT-PCR. RESULTS: Phylogenetic analysis revealed four viruses designated as Razi-HKM891, Razi-HKM892, Razi-HKM893 and Razi-HKM894. Deduced amino acid sequence comparison with other IBV genotypes, published in the GenBank database, indicated that the isolates Razi-HKM891 and Razi-HKM894 were placed into the pathogenic 793/B serotype. However, the isolates Razi-HKM892 and Razi-HKM893 were different with previously described isolates in Iran. The Razi-HKM893 is closely related to recently published isolates from countries in Middle East and likely indigenous to Iran. CONCLUSIONS: These findings is essential for improving the disease control strategies and thus emphasize the importance of continuous surveillance of the disease and of sharing the information to the global scientific community, which would help to fill the epidemiological gaps in the regions and to validate the robustness of diagnostic screening.
کلیدواژه‌ها [English]
', './data/jvr/coversheet/cover_fa.jpg'))