ارتباط مجتمع عمده پذیرش بافتی طیور با پاسخ پادتن به واکسن

نویسندگان

1 گروه میکروبیولوژی و ایمونولوژی، دانشکده دامپزشکی دانشگاه تهران، تهران-ایران

2 گروه پاتوبیولوژی، دانشکده دامپزشکی دانشگاه شیراز، شیراز-ایران

چکیده

زمینه مطالعه: مجتمع عمده پذیرش بافتی (MHC) نقشی مرکزی در چگونگی پاسخ به بیماریهای عفونی دارد و پاسخ‌های ایمنی تحت کنترل ژن‌های این مجتمع هستند. بدلیل تنوع MHC تفاوت‌های فردی در پاسخ به واکسن‌های مختلف طیور مشاهده می‌شود. بررسی ارتباط آلل‌های MHC طیور با توان پاسخ به واکسن‌های معمول، به توفیق واکسیناسیون و کنترل بیماریها کمک خواهد نمود. هدف: هدف از مطالعه حاضر بررسی تنوع MHC در جمعیت طیور بومی خراسان و ارتباط آن با پاسخ پادتن به واکسن‌های گامبورو، نیوکاسل و آنفلوانزا است. روش‌کار: به منظور تعیین ژنوتیپ‌های MHC از نشانگر ریزماهواره LEI0258 و روش تحلیل قطعه‌ای استفاده شد. عیار پادتن تولید شده بر ضد واکسن‌های نیوکاسل و آنفلوانزا با آزمون ممانعت هماگلوتیناسیون و واکسن گامبورو با آزمون الایزا اندازه‌گیری شدند. تحلیل آماری با کمک نرم‌افزار SPSS نسخه 21 صورت پذیرفت. جهت تعیین ضرایب تأثیر آلل‌ها از تحلیل رگرسیون تک متغیره با روش حداقل مربعات استفاده شد. نتایج: در جمعیت طیور بومی خراسان 13 آلل ریزماهواره LEI0258 شناسایی شد که نشان‌دهنده تنوع بالای MHC در این جمعیت است. آلل 361 بیشترین (48/28%) و آلل 350 کمترین (69/0%) فراوانی را در جمعیت داشتند. در بررسی ارتباط MHC با پاسخ ایمنی، آلل 266 ریزماهواره LEI0258 با کاهش عیار پادتن ضد واکسن گامبورو و آلل‌های 311 و 313 با افزایش پاسخ پادتن به واکسن نیوکاسل همراه بودند (05/0<p). نتیجه‌گیری‌نهایی: با توجه به نقش قابل ملاحظه MHC در مقاومت یا حساسیت در برابر بیماریهای عفونی ویروسی و کیفیت پاسخ‌های ایمنی، از روش‌ها و نتایج بدست آمده می‌توان برای انتخاب و بهبود جمعیت‌های در حال اصلاح نژاد بهره برد.
 

کلیدواژه‌ها

عنوان مقاله [English]

Study of the association of major histocompatibility complex with antibody response to vaccines in Khorasan native chickens

نویسندگان [English]

  • Gholamraza Nikbakhat Brujeni 1
  • Atefeh Esmailnejad 2
  • Neda Khazeni Oskoui 1
1 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran
2 Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz-Iran
چکیده [English]

BACKGROUND: Major histocompatibility complex (MHC) plays a central role in regulation and control of the immune responses to infectious diseases. Due to its polymorphism, individual differences in response to vaccines have been observed in different chicken populations. Studying the association of chicken MHC with immune response to vaccines will help the control of infectious disease and vaccination success. Objectives: The present study aimed to evaluate the MHC polymorphism and its association with antibody response against infectious bursal disease (Gumboro), Newcastle (ND) and Influenza (AI) vaccines in Khorasan native chickens. Methods: Diversity of LEI0258 microsatellite marker (MHC genotyping) was investigated by fragment analysis method. Antibody titer against IBD was measured by ELISA and antibody titers against ND and AI vaccines were measured by Haemaglutination Inhibition (HI) assay. Statistical analysis was performed using SPSS software (version 21). Univariate regression analysis was performed using weighted least squares with weight number of progeny mean data. Results: Total of 13 LEI0258 microsatellite alleles were identified in Khorasan native chickens which indicated a high genetic diversity in the population. The allele 361 bp had the highest (28.48%) and the allele 350 bp had the lowest (0.69%) frequency, respectively. In evaluating the association of MHC with immune responses, 311 and 313 bp alleles were significantly associated with elevated immune responses to Newcastle vaccine, while allele 266 bp was associated with lower IBDV antibody titers (p<0.05). ConclusionS: According to the important role of MHC in controlling infectious disease resistance or susceptibility and quality of immune responses, these results could be used for selection and improving the populations under selective breeding.
 

کلیدواژه‌ها [English]

  • Gumboro
  • influenza
  • major histocompatibility complex
  • Microsatellite
  • newcastle

مراجع

Bacon, L.D., Hunt, H.D., Cheng, H.H. (2001) Genetic resistance to Mareks disease. Curr Top Microbiol Immunol. 255: 121-141.
Bashalkhanov, S., Pandey, M., Rajora, O. (2009) A simple method for estimating genetic diversity in large populations from finite sample sizes. BMC. Genet. 10: 84.
Boonyanuwat, K., Thummabutra, S., Sookmanee, N., Vatchavalkhu, V., Siripholvat, V. (2006) Influences of major histocompatibility complex class I haplotypes on avian influenza virus disease traits in Thai indigenous chickens. Anim Sci J. 77: 285-289.
Bulut, Z., Kurar, E., Ozsensoy, Y., Nizamlioglu, M., Garip, M., Yilmaz, A., Caglayan, T., Dere, S., Kurtoglu, V., Dogan, M. (2013) Determination of chromosomal regions affecting body weight and egg production in Denizli X White Leghorn F2 populations. Eurasian J Vet Sci. 29: 30-38.
Bumstead, N. (1998) Genetic resistance to avian viruses. Rev Sci Technol. 17: 249-255.
Butter, C., Staines, K., Hateren, A., Davison, T.F., Kaufman, J. (2013) The peptide motif of the single dominantly expressed class I molecule of the chicken MHC can explain the response to a molecular defined vaccine of infectious bursal disease virus (IBDV). Immunogenetics. 65: 609-618.
Chang, C.S., Chen, C.F., Berthouly-Salazar, C., Chazara, O., Lee, Y.P., Chang, C.M., Chang, K.H., BedHom, B., Tixier-Biochard, M. (2011) A global analysis of molecular markers and phenotypic traits in local chicken breeds in Taiwan. Anim Genet. 43: 172-182.
Cheng, H.H. (2003) 21 Selection for disease resistance: Molecular genetic techniques. In: Poultry Genetics, Breeding, and Biotechnology. Muir, W.M., Aggrey, S.E. (eds.). (1st ed.) CABI Publishing, Wallingford, UK. p. 385-399.
Davison, T.T.F., Kaspers, B., Schat, K.A. (2008) Avian Immunology. (1st ed.) Elsevier Ltd. London, UK.
Ewald, S.J., Ye, X., Avendano, S., McLeod, S., Lamont, S.J., Dekkers, J.C. (2007) Associations of BF2 alleles with antibody titres and production traits in commercial pure line broiler chickens. Anim Genet. 38: 174-176.
Fulton, J.E., Juul-Madsen, H.R., Ashwell, C.M., McCarron, A.M., Arthur, J.A., O’Sullivan, N.P., Taylor Jr, R.L. (2006) Molecular genotype identification of the Gallus gallus major histocompatibility complex. Immunogenetics. 58: 407-421.
Hoffmann, I. (2009) The global plan of action for animal genetic resources and the conservation of poultry genetic resources. World Poult Sci J. 65: 286-297.
Hunt, H.D., Jadhao, S., Swayne, D.E. (2010) Major histocompatibility complex and background genes in chickens influence susceptibility to high pathogenicity avian influenza virus. Avian Dis. 54: 572-575.
Izadi, F., Ritland, C., Cheng KM. (2011) Genetic diversity of the major histocompatibility complex region in commercial and noncommercial chicken flocks using the LEI0258 microsatellite marker. Poult Sci. 90: 2711-2717.
Javanrouh Aliabad, A., Seyedabadi, H., Taheri Dezfuli, B. (2011) Association of insulin-like growth factor-I gene with body comosition traits in Iranian commercial broiler lines. World Appl Sci J. 14: 71-76.
Joiner, K.S., Hoerr, F.J., Van, S.E., Ewald, S.J. (2005) The avian major histocompatibility complex influences bacterial skeletal disease in broiler breeder chickens. Vet Pathol. 42: 275-281.
Juul-Madsen, H.R., Dalgaard, T.S., Rontved, C.M., Jensen, K.H., Bumstead, N. (2006) Immune response to a killed infectious bursal disease virus vaccine in inbred chicken lines with different major histocompatibility complex haplotypes. Poult Sci. 85: 986-998.
Lima-Rosa, C.A.d.V., Canal, C.W., Fallavena, P.R.V., Freitas, L.B.d., Salzano, F.M. (2005) LEI0258 microsatellite variability and its relationship to B-F haplotypes in Brazilian (blue-egg Caipira) chickens. Genet Mol Biol. 28: 386-389.
Luo, C., Qu, H., Ma, J., Wang, J., Li, C., Yang, C., Hu, X., Li, N., Shu, D. (2013) Genome-wide association study of antibody response to Newcastle disease virus in chicken. BMC Genet. 14: 42.
Lwelamira, J., Kifaro, G.C., Gwakisa, P.S., Msoffe, P.L.M. (2008) Association of LEI0258 microsatellite alleles with antibody response against Newcastle disease virus vaccine and body weight in two Tanzania chicken ecotypes. Afr J Biotechnol. 7: 714-720.
Miller, M.M., Bacon, L.D., Hala, K., Hunt, H.D., Ewald, S.J., Kaufman, J., Zoorob, R., Briles, W.E. (2004) 2004 Nomenclature for the chicken major histocompatibility (B and Y) complex. Immunogenetics. 56: 261-279.
Nikbakht, G., Esmailnejad, A., Barjesteh, N. (2013) LEI0258 Microsatellite Variability in Khorasan, Marandi, and Arian Chickens. Biochem Genet. 51: 341-349.
Norup, L.R., Dalgaard, T.S., Pedersen, A.R., Juul-Madsen, H.R. (2011) Assessment of Newcastle disease-specific T cell proliferation in different inbred MHC chicken lines. Scand J Immunol. 74: 23-30.
Notter, D.R. (1999) The importance of genetic diversity in livestock populations of the future. J Anim Sci. 77: 61-69.
Owen, J.P., Delany, M.E., Cardona, C.J., Bickford, A.A., Mullens, B.A. (2009) Host inflammatory response governs fitness in an avian ectoparasite, the northern fowl mite (Ornithonyssus sylviarum). Int J Parasitol. 39: 789-799.
Pitcovski, J., Cahaner, A., Heller, E.D., Zouri, T., Gutter, B., Gotfried, Y., Leitner, G. (2001) Immune response and resistance to infectious bursal disease virus of chicken lines selected for high or low antibody response to Escherichia coli. Poult Sci. 80: 879-884.
Schou, T.W., Labouriau, R., Permin, A., Christensen, J.P., Sorensen, P., Cu, H.P., Nguyen, V.K., Juul-Medsen, H.R. (2010) MHC haplotype and susceptibility to experimental infections (Salmonella Enteritidis, Pasteurella multocida or Ascaridia galli) in a commercial and an indigenous chicken breed. Vet Immunol Immunopathol. 135: 52-63.
Schou, T.W., Permin, A., Juul-Madsen, H.R., Sorensen, P., Labouriau, R., Nguyen, T.L., Fink, M., Pham, S.L. (2007) Gastrointestinal helminths in indigenous and exotic chickens in Vietnam: association of the intensity of infection with the major histocompatibility complex. Parasitology. 134: 561-573.
Sheldon, B.L. (2000) Research and development in 2000: Directions and priorities for the world’s poultry science community. Poult Sci. 78: 147-158.
Sironi, L., Williams, J.L., Stella, A., Minozzi, G., Moreno, A., Ramelli, P. Han, J., Weigend, S., Wan, J., Lombardi, G., Cordioli, P., Mariani, P. (2011) Genomic study of the response of chicken to highly pathogenic avian influenza virus. BMC Proc 5 Suppl 4: S25.
Suzuki, K., Matsumoto, T., Kobayashi, E., Uenishi, H., Churkina, I., Plastow, G., Yamashita, H., Hamasima, N., Mitsuhashi, T. (2010) Genotypes of chicken major histocompatibility complex B locus associated with regression of Rous sarcoma virus J-strain tumors. Poult Sci. 89: 651-657.
Tizard, I.R. (2009) Veterinary Immunology. (8th ed.) Saunders Elsevier Ltd. New York, USA.
Weigend, S., Matthes, S., Solkner, J., Lamont, S.J. (2001) Resistance to Marek’s disease virus in White Leghorn chickens: effects of avian leukosis virus infection genotype, reciprocal mating, and major histocompatibility complex. Poult Sci. 80: 1064-1072.
Weigend, S., Vef, E., Wesch, G., Meckenstock, E., Seibold, R., Ellendorff, F. (1995) Conception for conserving genetic resources in poultry in Germany. Archiv fuer Gefluegelkunde. 59: 327-334.
Yonash, N., Heller, E.D., Hillel, J., Cahaner, A. (2000) Detection of RFLP markers associated with antibody response in meat-type chickens: haplotype/genotype, single-band, and multiband analyses of RFLP in the major histocompatibility complex. J Hered. 91: 24-30.

فایل ها

هم رسانی

ارجاع به این مقاله

آمار