تأثیر اسانس مرزه خوزستانی بر pH پس از کشتار و پتانسیل آنتی اکسیداتیو عضله سینه مرغ گوشتی تحت تنش گرمایی

نویسندگان

1 گروه علوم دامی، دانشکده کشاورزی دانشگاه لرستان، خرم آباد-ایران

2 بخش بیوشیمی، دانشکده دامپزشکی دانشگاه لرستان، خرم آباد-ایران

3 لابراتوار تحقیقات کاربردی گیاهان دارویی خرمان، لرستان، خرم آباد-ایران

4 مرکز تحقیقات داروهای گیاهی رازی، دانشگاه علوم پزشکی لرستان، خرم آباد-ایران

چکیده

زمینه مطالعه: تأمین منابع آنتی اکسیدانی از طریق خوراک یا آب برای مرغ گوشتی ضمن کاهش اثرات زیانبار رادیکال‌های آزاد در بدن پرنده متأثر از تنش گرمایی، به افزایش پتانسیل آنتی اکسیداتیو و پایداری لیپیدها در بافت‌های مختلف کمک می‌کند. هدف: این مطالعه به منظور بررسی تأثیر اسانس مرزه خوزستانی (Satureja khuzistanica) بر تغییرات پس از کشتار pH و پتانسیل آنتی اکسیداتیو عضله سینه در مرغ گوشتی اجرا شد. روش کار: تعداد 720 قطعه جوجه یکروزه سویه آرین تهیه و اثر شش تیمار آزمایش شامل سطوح mg/L‌0، 200، 300، 400 و 500 اسانس مرزه خوزستانی و mg/L‌‌500 پلی سوربات-80 در آب آشامیدنی، در 6 تکرار (شامل 20 پرنده)، در قالب طرح بلوک‌های کامل تصادفی، طی 42 روز بررسی شد. فعالیت آنزیم کاتالاز (با استفاده از روش کینتیکی)، گلوتاتیون پراکسیداز و سوپراکسید دیسموتاز (با استفاده از کیت‌های راندوکس) بوسیله اسپکتروفتومتر اندازه گیری شد. میزان پراکسیداسیون لیپید با سنجش میزان مواد واکنش دهنده با تیوباربیتوریک اسید (TBARS) به روش شیمیایی در عضله سینه انجام گرفت. نتایج: وزن سینه، pH اولیه و نهایی عضله سینه مرغ در سن 42 روزگی، تحت تأثیر افزودن اسانس مرزه به آب آشامیدنی قرار نگرفت (05/0>p). سطوح بالاتر از mg/L‌200 اسانس مرزه، میزان TBARS موجود در عضله سینه را به طور معنی‌داری کاهش داد (05/0<p). فعالیت آنزیم کاتالاز در عضله سینه پرندگان دریافت کننده آب حاوی اسانس، بالاتر از گروه‌های شاهد بود. فعالیت آنزیم گلوتاتیون پراکسیداز در عضله سینه مرغ‌های دریافت کننده سطوح mg/L‌400 و 500  اسانس مرزه به طور معنی‌داری بالاتر از مرغ‌های دریافت کننده آب فاقد اسانس بود (05/0<p). افزودن اسانس مرزه به آب تا سطح mg/L‌400 باعث افزایش میزان فعالیت آنزیم سوپراکسید دیسموتاز عضله سینه گردید (05/0<p). نتیجه گیری نهایی: افزودن اسانس مرزه خوزستانی به آب آشامیدنی مرغ گوشتی تحت تنش گرمایی، با افزایش فعالیت آنزیم‌های آنتی اکسیدان و کاهش پراکسیداسیون لیپید، موجب افزایش پتانسیل آنتی اکسیداتیو عضله سینه مرغ می‌شود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Satureja khuzistanica essential oils on antioxidative potential and postmortem pH of breast muscle in heat stressed broiler chicken

نویسندگان [English]

  • Heshmatollah Khosravinia 1
  • Masoud Alirezaei 2
  • Sedigheh Ghasemi 3
  • Shima Neamati 4
1 1Department of Animal Sciences, Faculty of Agriculture, Lorestan University, Khorramabad-Iran
2 Division of biochemistry, Faculty of Veterinary Medicine, Lorestan University, Khorramabad-Iran 3Lorestan Medical Plant Lab, Khorraman, Khorramabad-Iran
3 Lorestan Medical Plant Lab, Khorraman, Khorramabad-Iran
4 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad-Iran
چکیده [English]

BACKGROUND: Inclusion of antioxidant sources in feed or water reduces the detrimental effects of free radicals and increases the antioxidative potential and stability of lipids in heat stressed broiler chicken. OBJECTIVES: This study was conducted to examine the effect of Satureja khuzistanica essential oils (SkEO) on early postmortem and ultimate pH as well as antioxidative potential of breast muscle in broiler chicken. METHODS: A total number of 720,1-d-old Arian broiler chicks were used in a 42-d trial to examine the effects of six experimental treatments consisting inclusion of 0 (control-), 200, 300, 400, 500 mg/L SkEO and 500 mg/L Polysorbate-80 (control+) in drinking water in six replicates of 20 birds each in a completely randomized blocks design. Catalase enzyme activity (by kinetic method) and glutathione peroxidase as well as superoxide dismutase activities (via Randox® kits) were measured spectrophotometerically. Lipid peroxidation also was measured in breast muscle by a chemical method with thiobarbituric acid. RESULTS: The breast percentage and early as well as ultimate pH of breast muscle was not differ for the birds received SkEO-treated water compared to the control birds (p>0.05). Addition of SkEO in drinking water at doses greater than 200 mg/L significantly decreased the thiobarbituric acid reactive substances values in breast muscle of the birds in comparison with the control birds (p<0.05). Catalase activity was significantly higher in breast muscle of the SkEO-treated groups than the control groups (p<0.05). Activity of glutathione peroxidase was significantly greater in the birds given 400 and 500 mg/L SkEO. Addition of SkEO into water up to 400 mg/L increased superoxide dismutase activity in breast muscle of the treated birds compared to the control birds. CONCLUSIONS: It was concluded that supplementation of drinking water with SkEO elevates the antioxidative potential and increases the lipid stability of breast muscle in heat stressed male broiler chicken.

کلیدواژه‌ها [English]

  • Breast muscle
  • heat stress
  • phytogenic antioxidants
  • Satureja khuzistanica
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