مطالعه هیستومتری فولیکول های تخمدان موش های ماده تحت درمان با متیل فنیدیت

نویسندگان

1 گروه آناتومی، واحد علوم پزشکی تهران دانشگاه آزاد اسلامی، تهران- ایران

2 گروه چشم پزشکی دانشکده پزشکی، دانشگاه آزاد اسلامیواحد علوم پزشکی تهران ،ایران

3 گروه علوم پایه، دانشکده دامپزشکی دانشگاه تهران، تهران- ایران

4 گروه اطفال، دانشگاه علوم پزشکی زنجان، تهران- ایران

چکیده

زمینه مطالعه: بیشترین بخش تخمدان را فولیکولها تشکیل میدهند که بعضی از داروها میتوانند موجب تغییراتی در آن شوند. هدف:  مطالعه تخمدان موشهای بالغی است که در دوران رشد از متیل فنیدیت (Methylphenidate) استفاده کردهاند. روش کار: 40 سر موش سوری ماده نابالغ، در سن سه هفتگی به یک گروه کنترل و سه گروه تجربی تقسیم شدند. حیوانات در گروههای تجربی، به روش خوراکی به مدت 60 روز، متیل فنیدیت را به میزان mg/kg 2، 5 و10 دریافت کردند. پس از اتمام مدت تیمار و تعیین وزن مجدد، حیوانات را بیهوش و سطح سرمی ‌FSH و LH تعیین گردید و تغییرات ساختاری فولیکولهای تخمدانی و اجسام زرد آنها مورد مطالعه قرار گرفت. نتایج:  میانگین اختلاف وزن بدن در گروههای تجربی نسبت به گروه کنترل کاهش معنیداری را نشان داد (05/0>p). همچنین در مقایسه انواع فولیکولهای تخمدانی در گروههای تجربی نسبت به گروه کنترل کاهش معنیداری در فولیکولهای  پیش آنترال، آنترال وجسم زرد، افزایش معنیداری در فولیکولهای آترزی مشاهده گردید(05/0>p). نتیجهگیرینهایی: استفاده طولانی مدت متیل فنیدیت در موش موجب افزایش فولیکولهای آترزی، کاهش جسم زرد، در نتیجه کاهش رشد تعدادی از فولیکولها و اووسیت و تغییر در بعضی از سلولهای لوتئینی  میشود.

کلیدواژه‌ها


عنوان مقاله [English]

Histometrical study of ovarian follicles of immature mice treated with methylphenidate

نویسندگان [English]

  • Simin Fazelipour 1
  • Farhad Adhami Moghadam 2
  • Parivash Davudi 1
  • Zahra Tootian 3
  • Fardin Assadi 4
1 Department of Anatomy, Tehran Medical Sciences Branch, Islamic Azad University, Tehran-Iran
2 Department of Ophtalmology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran-Iran
3 Department of Basic Sciences, Faculty of Veterrinary Medicine, University of Tehran, Tehran-Iran
4 Department of Pediatrics, University of Medical Sciences, Zanjan-Iran
چکیده [English]

BACKGROUND: The main part of ovary is consisted of follicles which certain drugs may cause change in them. OBJECTIVES: The purpose of this study was to investigate the effects of Methylphenidate on ovarian follicle of mice, treated by Ritalin before puberty. METHODS: 40 immature female mice at 3 weeks of age were divided into 4 groups, consisting of one control and 3 experimental groups. The experimental groups were gavaged by 2, 5 and 10 mg/kg methylphenidate respectively and the control group received only distilled water with the same method for 60 days. At the end of the experiment, the mice were weighed and then the serum levels of FSH and LH were assessed and structural changes of ovarian follicles and corpora lutea were studied. RESULTS: The mean difference of body weight in experimental groups compared with the control group which showed a significant reduction (p<0.05). In experimental groups compared with the control group, a significant reduction in pre enteral, enteral follicles, corpora lutea and a significant increase in atretic follicles were observed (p<0.05). CONCLUSIONS: Ritalin intake for a long period may increase the number of atretic follicles and decrease corpora lutea, so subsequently results in reduction of the growth of follicles and oocytes as well as inducing the atypical appearance of the cells in the luteinized cells.

کلیدواژه‌ها [English]

  • methylphenidate
  • mice
  • ovarian follicles
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