مقایسه تأثیرجیره تکمیلی (فلاشینگ) وeCG برمیزان باروری وچند قلوزایی میش های نژاد زل درتابستان

نویسنده

گروه علوم درمانگاهی، دانشکده دامپزشکی واحد گرمسار، دانشگاه آزاد اسلامی گرمسار، سمنان-ایران

چکیده

زمینه مطالعه: با توجه به متفاوت بودن میزان چند قلوزایی در نژادها و مناطق جغرافیای مختلف و ضرورت مطالعه عوامل مؤثر بر چند قلوزایی در نژادهای مختلف یافتن بهترین روش ایجاد چندقلو زایی وایجادفعالیت تخمدانی در خارج از فصل در هر نژاد میش ضروری می‌نماید. هدف: تعیین میزان فعالیت تخمدانی و چند قلوزایی در تابستان و مقایسه اثرجیره و eCG در میزان باروری وچند قلو زایی میش‌های نژاد زل. روش کار: دراوایل تابستان 135رأس میش زل بصورت تصادفی در6 گروه مشابه به قرار ذیل تقسیم شدند. گروه 1(22= N) میش‌ها به مدت 21 روزقبل ازقوچ اندازی علاوه برچرای آزاد از جیره تکمیلی بهره مندگردیدند. گروه 2(22=N) میش‌ها 8 روز قبل از قوچ اندازی جیره تکمیلی مصرف کردند. گروه 3 (26=N) میش‌ها جیره تکمیلی از 8 روز قبل از قوچ اندازی وهمچنین اسفنج واژنی پروژسترون دار و در هنگام‌خروج‌اسفنج، IU  400 eCG دریافت کردند. گروه 4 (23=N) میش‌ها در این گروه هیچ گونه جیره تکمیلی دریافت نداشتند ولی اسفنج واژنی پروژسترون و eCG در هنگام خروج اسفنج دریافت کردند. گروه 5 (20=N)  میش‌ها جیره تکمیلی و هورمون eCG دریافت نداشتند و تنها فحلی آنها با اسفنج واژنی همزمان گردید. گروه 6 (22=N)  به عنوان گروه شاهد در نظر گرفته شد و میش‌ها در این گروه تحت هیچگونه تیماری قرار نگرفتند. همزمانی فحلی درکلیه میش‌ها بجزگروه شاهد(6) بوسیله اسفنج داخل واژنی آغشته به پروژسترون برای مدت 14 روزصورت پذیرفت. تغذیه پایه کلیه دام‌ها براساس چرای آزاد در مرتع با پوشش غالب ازگیاهان خانواده گندمیان صورت پذیرفت. میش‌های گروه‌های آزمایشی به منظورجفت‌گیری 48 ساعت پس ازخروج اسفنج به مدت 72 ساعت درمجاورت قوچ بارورقرارگرفتند. نتایج: یافته‌ها نشان دادمیزان باروری متعاقب همزمانی فحلی درگروه سوم ازگروه دوم و پنجم بصورت معنی‌داری بیشتر بود ولی در دیگر گروه‌ها تفاوت معنی‌داری نداشت (05/0<p). شاخص prolificacy  وشاخص fecundityدرگروه‌های درمان شده باeCG به‌طور معنی‌داری از دیگر گروه‌ها بیشتر بود (0.01>p) ولی جیره تکمیلی بر روی آن تأثیر معنی‌داری بر جا نگذاشت (05/0<p). نتیجه گیری نهایی: از این مطالعه نتیجه‌گیری شداستفاده از پروژسترون واژنی و eCG در فصل تابستان در نژاد زل در افزایش تعداد چند قلوزایی مؤثر است جیره تکمیلی افزایش معنی‌داری در آن ایجاد نمی‌کند.

کلیدواژه‌ها


عنوان مقاله [English]

A comparison between the Effects of supplementary feeding and eCG on fertility and multiple ovulation rate in Zel ewes during summer

نویسنده [English]

  • Majid Mohammad Sadegh
Department of Clinical Sciences, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Semnan-Iran
چکیده [English]

BACKGROUND: Considering the difference between the rate of multiple ovulation and ovarian activities of different breeds of ewes in different regions, especially out of seasonal breeding condition, finding efficient and effective protocols to induce multiple ovulation out of seasonal breeding would be much appreciated. OBJECTIVES: This study was conducted to determine fertility and multiple ovulation rates during summer and investigate the effect of supplementary feeding, and eCG injection prior to mating in the reproductive performance of Zel ewes. METHODES: At the beginning of summer, 135 ewes in Savadkooh region (north of Iran) were randomly selected and divided into six groups. The estrus cycles of ewes, except in control groups, were synchronized using a vaginal sponge for 14 days. In groups 1 (n=22) and 2 (n=22) ewes were fed with supplementary feeding for 21 and 8 days, respectively, prior to mating. In Group 3 (n=26) ewes were fed with the same ration as Group 2 and were injected (IM) with 400 IU eCG at the time of sponge withdrawal. Ewes in Group 4 (n=23) just grazed on the pasture and injected with eCG as Group 3. In Group 5 (n=20) the estrus of ewes was synchronized as other groups, but they were not received any other treatment. Ewes in Group 6 (n=22, Control) received no treatment, and as the same Group 1 and 2 were mated with fertile rams for eight weeks. Experimental ewes were mated with fertile rams (1:8) 48 hrs after sponge removal for 96 hrs. RESULTS: The findings showed that the fertility in group 3 was significantly more than group 2 and 5, but there was no significant difference between the others (p>0.05). The prolificacy and fecundity were significantly higher in groups treated with eCG than the others (p<0.01), however there was no significant difference between groups 3 (with flashing) and 4 (without flashing). CONCLUSIONS: It could be concluded that vaginal sponge and eCG treatment may improve the multiple lambing and reproductive performance of Zel ewes during summer, and supplementary feeding prior to mating, as described in this experiment, cannot make any significant improvement in multiple lambing.

کلیدواژه‌ها [English]

  • eCG
  • flashing
  • vaginal progesterone
  • Zel ewes
Alkass, J.E., Hamra, A.H., Ibrahim, F.F. (1989) Combined effect of flushing and hormonal treatment on the reproductive performances of Awassi ewes. Indian J Anim Sci. 59: 1249-1252.
Barrett, D.M.W.,  Bartlewskib, P.M., Batista-Arteagac, M.,  Symingtona, A., Rawlingsa, N.C. (2004) Ultrasound and endocrine evaluation of the ovarian response to a single dose of 500 IU of eCG following a 12-day treatment with progestogen-releasing intravaginal sponges in the breeding and nonbreeding seasons in ewes. Theriogenology. 61: 311–327.
Cline, M.A., Raltson, J.N., Seals, R.C., Lwis G.S. (2001) Intervals from norgestomet withdrawal and injection of equine chorionic gonadotrophin or P.G. 600 to estrus and ovulation in ewes. J Anim Sci. 79: 589-594.
Crocker, K.P., Johnx, M.A., Bell, S.H., Brown, G.A., Wallace, J.F. (1990) The influence of vaccination with f ECUNDIN and supplementation with lupin grain on the reproductive performance of Merino ewes in Western Australia. Aust J Experimental Agric. 30: 469-476.
Cummins, L.J., Spiker, S.A., Cook, C., Cox, R.I. (1984) The effect of steroid immunization of ewes and their nutrition on the ovulation rate and associated reproductive wastage. In: Reproduction in Sheep. Lindsy, D.R., Pearce, C. (eds.). Australian academy of science. Canberra, Australia. p. 326-328.
Davis, I.F., Brien, F.D., Findley, J.K., Cuming, I.A. (1981) Interaction between dietery protein, ovulation rate and follicle stimulating hormone level in the ewe. Anim Reprod Sci. 4: 19-28.
Dowing J.A., Joss, J., Connel, P., Scaramuzzi, R.J. (1995) Ovulation rate and the concentration of gonadotrophic and metabolic hormones in ewes fed lupin grin. J Reprod Fertil Dev. 103: 137-145.
Enzminger, M.E., Parker, R.O. (1986) Reproduction in sheep and goats. In: Sheep and Goat Science. The interstate Printers and publishers, Danville, USA. p. 349-367.
Fleisch, A., Werne, S., Heckendorn, F., Hartnack, S., Piechotta, M., Bollwein, H., Thun, R., Janett, F. (2012) Comparison of 6-day progestagen treatment with Chronogest CR and Eazi-breed™ CIDR G intravaginal inserts for estrus synchronization in cyclic ewes. Small Rumin Res. 107: 141–146.
Fell, H. (1988) Intensive Sheep Management. (2nd ed.) Farming Press, Australia. p. 165-176.
Henderson, D. (1985) Control of breeding season in sheep and goats, In Practice, (July), 5: 118-123.
Helali, I, Abdel-Hakim, N.F., Safwat, M., Salama, R. (1990) Effect of age and level of nutrition on fertility and productivity in Barki ewes, Egypt. J Anim Pord. 27: 241-258.
Jabbour, Hn., Ryan, Jp., Evans, G., Maxwell, W.M.C. (1991) Effects of season GnRH administration and lupin supplementation on ovarian adnendocrine responses of Merino ewes treated with ECG and FSH –P to induce superovulation. Reprod Fert Develop. 55: 699-707.
Kermani Moakhar, H.,  Kohram, H.,  Zareh Shahneh, A.,  Saberifar, T. (2012) Ovarian response and pregnancy rate following different doses of eCG treatment in Chall ewes. Small Rumin Res. 102: 63-67.
Khaldari, M., Tajik, P., Afzalzadeh, A., Farzin, N. (2004) Efficacy of CIDR and eCG estrous synchronization and twining rate in Zandi ewes during the breeding season. J Vet Res (University of Tehran). 59: 141-145.
Martemucci G., D’Alessandro, A.G. (2010) Estrous and fertility responses of dairy ewes synchronized with combined short term GnRH, PGF2α and estradiol benzoate treatments. Small Rumin Res. 93: 41-47.
Mousavy, S.M., Sookhtehzari, A., Vojgani, M. (2009) Efficacy of GnRH or CIDR on out of season reproductive performance of ewe. J Vet Res (University of Tehran). 64: 69-72.
Mylne, M.J.A., Hunton, J.R., Buckrll, B.C. (1996) Artificial insemination of sheep. In: Current Therapy in Large Animal Theriogenology.  Youngquist, R.S. (ed.). WB Saunders  Company. Philadelphia USA. p. 585-594.
Nottle, N.B., Hynd, P.I., Seamark, R.F., Setchell, B.P. (1988) Increase of ovulation in lupin fed ewes are initiated by increase in protein digested post rumially. J Reprod Fertil. 84: 563-566.
Nottle, N.B., Seamark, R.F., Setchell, B.P. (1990) Feeding lupine grain for six days prior to a Cloprostenol induced luteolysis to increase ovulation rate in sheep irrespective of when in oestrous cycle supplementation commences. J Reprod Fertil Dev. 2: 189-192.
Nottle, N.B., Kleeman, Do., Grosser, T.I., Seamark, R.F. (1997) Evaluation of nutrition strategy to increase ovulation rate in Merino ewes mated in late spring-early summer. Anim Reprod Sci. 47: 255-261.
Oldham, C.M., Lindsay, D.R. (1984) The minimum period of Intake of lupin grain required by ewes to increase their ovulation rate when gazing dry summer pasture. Reproduction in sheep, Cambridge University Press. Cambridge, USA. p. 274-276.
Omidi, M., Jillella, D., Cameron, R.D., Geddes, P.H., Young, M. (1996) The effect of lupin supplementation on superovulatory response and embryo recovery in merino ewes. Theriogenology. 45: 321.
Rhind, S.M., Leslie, I.D., Gunn, R.G., Doney, J.M. (1985) Plasma FSH, LH, Prolactin and Progesterone profile of Cheviot ewes with different levels of intake before and after mating and associated effects on reproductive performance.Anim Reprod Sci. 8: 301-313.
Sadeghipanah, H.L., Zare Shahneh, A.L., Nik-Khah, A.L., Niasari Nasa[ii, A. (2006) Effects of Different Supplemental Fats to Flushing Diet on Reproduction of Zandi Ewes. J Vet Res (University of Tehran). 61: 101-106.
Sawalha, M.N., Kridli, R.T, Jawasreh, K.I., Meza-Herrera, C.A.  (2011) The use of melatonin and progestagen-eCG to initiate reproductive activity in prepuberal Awassi ewe lambs. Trop Anim Health Prod. 43: 1345-50.
Schilo, K.K. (1992) Effect of dietary Energy on control of luteinizing hormone secretion in cattle and sheep. J Anim Sci. 70: 1580-1593.
Sirjani, M.A., Kohram, H., Shahir, M.H. (2012) Effects of eCG injection combined with FSH and GnRH treatment on the lambing rate in synchronized Afshari ewes. Small Rumin Res. 106: 59-63.
Sookhthezary, A., Vojgani,M., Niassari-Naslaji, A. (2006) Survey on Efficacy of Melatonin Implant in the Atabi Rams on Improvement of Reproductive Performance.in the Anestrus Ewes. J Vet Res (University of Tehran). 61: 181-185.
Szell, A., Mcleod, I.M., Windsor, D.P., Kelly, R.W. (1994) Productionof identical twins lambs by embryo splitting. Theriogenology. 41: 1643-1652.
Teleni, E., Rowe, J.B. (1986) Ovulation of ewes, J Agric, W Aust. 27: 36-38.
Uslu, B.A., Tasal, T., Gulyuz, F., Sendag, S., Ucar, O., Goericke-Pesch, S., Wehrend, A., (2012) Effects of oestrus synchronisation using melatonin and norgestomet implants followed by eCG injection upon reproductive traits of fat-tailed Morkaraman ewes during suckling, anoestrus season. Small Rumin Res. 108: 102–106.s