بررسی تأثیرعصاره گیاهان جغجغه، تاتوره و استبرق بر سه گونه باکتری بیماریزای ماهی

نویسندگان

پژوهشکده تالاب بین المللی هامون، گروه شیلات، دانشگاه زابل، زابل- ایران

چکیده

زمینه مطالعه:  استفاده مکرر از آنتی بیوتیک‌ها در زمینه‌های مختلف (دامپزشکی و پزشکی) ظهور و بروز پدیده مقاومت را در باکتری‌های بیماریزا بهبود می‌بخشد. با توجه به مشکل مقاومت میکروبی، نیاز فوری به کشف داروهای جدید و درمان‌های جایگزین برای کنترل بیماریهای باکتریایی در زمینه آبزی پروری وجود دارد. هدف: هدف از این مطالعه، بررسی اثرات ضد باکتریایی عصاره‌های متانولی و هگزانی گیاهان دارویی جغجغه (Prosopis farcta)، تاتوره(Datura stramunium L) و استبرق (Calotropis procera L)، بر باکتری‌های بیماریزای مهم ماهی از جمله آئروموناس هیدروفیلا (Aeromonas hydrophilaیرسینیا راکری (Yersinia ruckeri) و استرپتوکوکوس اینیایی (Streptococcus iniae) است. روش کار: عصاره گیری با استفاده از دستگاه روتاری(تقطیر در خلا) انجام شد. برای تعیین حداقل غلظت مهار رشد (MIC) از روش استاندارد رقیق سازی در محیط مایع استفاده شده و میزان حداقل غلظت باکتری کشی(MBC) با استفاده از مقادیر MIC هر عصاره تعیین شد. نتایج: نتایج نشان داد که قو ی ترین عصاره، عصاره متانولی میوه جغجغه بر هر سه باکتری مورد مطالعه با میزان MIC ، MBC، به ترتیب mg/mL‌25،50 بود. حساس ترین باکتری نسبت به عصاره‌های متانولی و هگزانی گیاهان، باکتری استرپتوکوکوس اینیایی بوده و باکتری آئروموناس هیدروفیلا و یرسینیا راکری مقاوم بودند و عصاره‌های مورد بررسی، خواص ضد باکتریایی قوی تری بر باکتری گرم مثبت در مقایسه با باکتری‌های گرم منفی داشتند. نتیجه گیری نهایی: بر طبق نتایج به دست آمده به نظر می‌رسد که استفاده از عصاره متانولی میوه جغجغه برای درمان بیماریهای باکتریایی در آبزی پروری مؤثر باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of plant extracts Prosopis farcta, Datura stramunium and Calotropis procera Against three species of Fish Pathogenic Bacteria

نویسندگان [English]

  • Narjes Sanchooli
  • Mahin Rigi
Department of Fisheries, Institute Research of International Hamoun Wetland, University of Zabol, Zabol-Iran
چکیده [English]

BACKGROUND: The repetitive use of antibiotics in different fields (veterinary and medicine) improves the emergence and occurrence of the resistance phenomenon in pathogenic bacteria. Due to the problem of antimicrobial resistance, it is an urgent need to discover new drugs and alternative treatments for the control of bacterial diseases in aquaculture. OBJECTIVES: The purpose of this study is to investigate antibacterial effects of methanol and hexane extracts of medicinal plants Rattles (Prosopis farcta), datura (Datura stramunium L) and milkweed (Calotropis procera L), the major pathogenic bacteria of fish, including Aeromonas hydrophila, Yersinia ruckeri and Streptococcus iniae. METHODS: Extraction was performed using a rotary evaporator. To determine the minimum inhibitory concentration (MIC), the standard microdilution method (Dilution in broth) was used and the minimum bactericidal concentration (MBC) was determined based on MIC values obtained for each extract. RESULTS: The results showed that the effect of most potent extract, methanol extract obtained from fruit rattle on the three studied bacteria, with MIC and MBC are 25, 50mg/ml, respectively. The most sensitive bacteria to methanol and hexane plant extracts, is bacterium Streptococcus iniae and Aeromonas hydrophila and Yersiniaruckeri bacteria were resistant. The studied extracts had stronger antibacterial properties against gram-positive bacteria compared to gram-negative. CONCLUSIONS: According to the results, it seems that the use of methanol extract of Prosopis farcta fruit is effective for treatment of bacterial diseases in aquaculture.

کلیدواژه‌ها [English]

  • Antibacterial activity
  • hexane extract
  • methanol extract
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