بررسی تأثیر ملاتونین بر روی قطر بیضه و خصوصیات منی در فصل غیر تولید مثلی در قوچ نژاد لری بختیاری

نویسندگان

1 گروه فیزیولوژی دام، دانشکده علوم دامی وصنایع غذایی دانشگاه کشاورزی ومنابع طبیعی رامین، اهواز، خوزستان- ایران

2 گروه علوم دامی، دانشکده علوم دامی وصنایع غذایی دانشگاه کشاورزی ومنابع طبیعی رامین، اهواز، خوزستان- ایران

3 گروه علوم درمانگاهی، دانشکده دامپزشکی دانشگاه لرستان، خرم آباد- ایران

چکیده

زمینه مطالعه: به منظور بررسی تأثیر ملاتونین بر تغییرات کمی وکیفی منیو افزایش بازده تولید مثلی در قوچ لری بختیاری در فصل غیر تولیدمثلی این مطالعه انجام شده است. هدف: هدف از این مطالعه، بررسی تأثیر ملاتونین روی قطر بیضه وتغییرات کمی و کیفی منی در فصل غیر تولیدمثلی درقوچ نژاد لری بختیاری بوده است. روش کار: این آزمایش در قالب طرح کاملاًً تصادفی در 8 رأس (4 رأس گروه کنترل و 4 رأس گروه درمان) قو چ نژاد لری ‏بختیاری به‌مدت 10 هفته در بهار 1392 صورت پذیرفت. قوچ‏های گروه درمان هر کدام 3 عدد قرص ملاتونین 18 میلی‏گرمی را به شکل کاشتنی در زیر قاعده گوش بعد از گذشت 5 هفته از شروع آزمایش تا پایان دوره دریافت نمودند. نتایج: بررسی شاخص‏های منی و اندازه‏گیری قطر بیضه به‌صورت هفتگی صورت گرفت. هیچگونه تفاوت معنی‏داری در مورد شاخص‏های اندازه‏گیری شده بین گروه کنترل وگروه درمان قبل از کاشت ملاتونین مشاهده نگردید. بعد از کاشتن ملاتونین، تفاوت معنی‏داری در میانگین قطر بیضه بین دو گروه درمان (025cm/0 ±50/33) و کنترل (cm 07/0± 67/30) و در حجم منی بین دو گروه درمان (03ml/0 ± 34/1) و کنترل (ml 04/0 ± 87/0) ‏و در غلظت اسپرم بین دو گروه درمان (05/0± 76/1) و کنترل (04/0± 37/1) میلیارد در هر میلی‏لیترمشاهده گردید (05/0<p). ولی در سایر پارامتر‏هااز قبیل میانگین درصد اسپرم‏های‏زنده، میانگین حرکات ‏پیش‏رونده‏اسپرم، میانگین درصد حرکات ‏تجمعی‏اسپرم و میانگین درصد ‏ناهنجاری‏اسپرم تفاوت معنی‏داری بین دوگروه‏ مشاهده نگردید (05/0>p). نتیجه‏گیری‌نهایی: نتایج این آزمایش نشان داد که استفاده از ملاتونین در فصل غیر تولیدمثلی سبب افزایش قطر بیضه، حجم منی وغلظت اسپرم‏در قوچ نژاد لری بختیاری شد ولی بر روی بقیه پارامترهای مورد آزمایش درقوچ‏های این نژاد بومی ایران اثری نداشت.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of melatonin on testicular circumference and semen characteristics in non-breeding season in Lori-Bakhtiari ram

نویسندگان [English]

  • Jamshid Fazli-nezad 1
  • Morteza Mamoeii 2
  • Arash Kheradmand 3
  • Ali Sookhtezary 3
1 Department of Animal Physiology, School of Animal and Food Science, Agriculture University of Ramin, Ahwaz- Iran
2 Department of Animal Sciences, School of Animal and Food Science, Agriculture University of Ramin, Ahwaz- Iran
3 Department of Clinical Sciences, School of Veterinary Medicine, Lorestan University, Khorram Abad- Iran
چکیده [English]

BACKGROUND: This study was conducted to evaluate the effect of melatonin on semen quality and quantity changes and increase of reproductive efficiency in Lori-Bakhtiari ram in the non-reproductive season. OBJECTIVES: The purpose of this study was to investigate the effect of melatonin on testicular size and semen qualitative and quantitative changes in the non-breeding season is Lori-Bakhtiari rams. METHODS: This study was designed to evaluate the effects of melatonin on the quantity and quality of semen and testicular diameter in 8 Lori- Bakhtiari rams (4 control rams and 4 treatment rams) for 10 weeks during spring 2013. Each ram in the treatment group was received three tablets of 18 mg implantable melatonin subcutaneously at the base of ear after 5 weeks from the start of the trial. RESULTS: Semen parameters and testicular diameter measurements were evaluated on a weekly basis. No significant differences were observed between 2 groups before melatonin implanting. A significant difference was observed after melatonin implanting between 2 groups in the mean of testicular diameter (treatment: 33.5±0.25 and control: 30.67±0.07 cm), in semen volume (treatment: 1.34±0.03 and control: 0.87± 0.04 ml) and in sperm concentration (treatment: 1.76±0.05 and control: 1.37±0.04 109/ml) (p<0.05). However, other parameters including average of lives sperm percentage, total sperm motility, sperm progressive movement and percentage of abnormal sperm were not significant (p>0.05). CONCLUSIONS: The results of this experiment showed that the use of melatonin in the non-reproductive season resulted in increase of testicular size, semen volume and sperm concentration in Lori-Bakhtiari ram. However, the rest of the semen parameters have not been affected.

کلیدواژه‌ها [English]

  • Lori-Bakhtiari ram
  • melatonin
  • non breeding season
  • semen evaluation
  • testicular diameter
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