بررسی تأثیر ملاتونین بر روی قطر بیضه و خصوصیات منی در فصل غیر تولید مثلی در قوچ نژاد لری بختیاری

نویسندگان

1 گروه فیزیولوژی دام، دانشکده علوم دامی وصنایع غذایی دانشگاه کشاورزی ومنابع طبیعی رامین، اهواز، خوزستان- ایران

2 گروه علوم دامی، دانشکده علوم دامی وصنایع غذایی دانشگاه کشاورزی ومنابع طبیعی رامین، اهواز، خوزستان- ایران

3 گروه علوم درمانگاهی، دانشکده دامپزشکی دانشگاه لرستان، خرم آباد- ایران

چکیده

زمینه مطالعه: به منظور بررسی تأثیر ملاتونین بر تغییرات کمی وکیفی منیو افزایش بازده تولید مثلی در قوچ لری بختیاری در فصل غیر تولیدمثلی این مطالعه انجام شده است. هدف: هدف از این مطالعه، بررسی تأثیر ملاتونین روی قطر بیضه وتغییرات کمی و کیفی منی در فصل غیر تولیدمثلی درقوچ نژاد لری بختیاری بوده است. روش کار: این آزمایش در قالب طرح کاملاًً تصادفی در 8 رأس (4 رأس گروه کنترل و 4 رأس گروه درمان) قو چ نژاد لری ‏بختیاری به‌مدت 10 هفته در بهار 1392 صورت پذیرفت. قوچ‏های گروه درمان هر کدام 3 عدد قرص ملاتونین 18 میلی‏گرمی را به شکل کاشتنی در زیر قاعده گوش بعد از گذشت 5 هفته از شروع آزمایش تا پایان دوره دریافت نمودند. نتایج: بررسی شاخص‏های منی و اندازه‏گیری قطر بیضه به‌صورت هفتگی صورت گرفت. هیچگونه تفاوت معنی‏داری در مورد شاخص‏های اندازه‏گیری شده بین گروه کنترل وگروه درمان قبل از کاشت ملاتونین مشاهده نگردید. بعد از کاشتن ملاتونین، تفاوت معنی‏داری در میانگین قطر بیضه بین دو گروه درمان (025cm/0 ±50/33) و کنترل (cm 07/0± 67/30) و در حجم منی بین دو گروه درمان (03ml/0 ± 34/1) و کنترل (ml 04/0 ± 87/0) ‏و در غلظت اسپرم بین دو گروه درمان (05/0± 76/1) و کنترل (04/0± 37/1) میلیارد در هر میلی‏لیترمشاهده گردید (05/0<p). ولی در سایر پارامتر‏هااز قبیل میانگین درصد اسپرم‏های‏زنده، میانگین حرکات ‏پیش‏رونده‏اسپرم، میانگین درصد حرکات ‏تجمعی‏اسپرم و میانگین درصد ‏ناهنجاری‏اسپرم تفاوت معنی‏داری بین دوگروه‏ مشاهده نگردید (05/0>p). نتیجه‏گیری‌نهایی: نتایج این آزمایش نشان داد که استفاده از ملاتونین در فصل غیر تولیدمثلی سبب افزایش قطر بیضه، حجم منی وغلظت اسپرم‏در قوچ نژاد لری بختیاری شد ولی بر روی بقیه پارامترهای مورد آزمایش درقوچ‏های این نژاد بومی ایران اثری نداشت.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of melatonin on testicular circumference and semen characteristics in non-breeding season in Lori-Bakhtiari ram

نویسندگان [English]

  • Jamshid Fazli-nezad 1
  • Morteza Mamoeii 2
  • Arash Kheradmand 3
  • Ali Sookhtezary 3
1 Department of Animal Physiology, School of Animal and Food Science, Agriculture University of Ramin, Ahwaz- Iran
2 Department of Animal Sciences, School of Animal and Food Science, Agriculture University of Ramin, Ahwaz- Iran
3 Department of Clinical Sciences, School of Veterinary Medicine, Lorestan University, Khorram Abad- Iran
چکیده [English]

BACKGROUND: This study was conducted to evaluate the effect of melatonin on semen quality and quantity changes and increase of reproductive efficiency in Lori-Bakhtiari ram in the non-reproductive season. OBJECTIVES: The purpose of this study was to investigate the effect of melatonin on testicular size and semen qualitative and quantitative changes in the non-breeding season is Lori-Bakhtiari rams. METHODS: This study was designed to evaluate the effects of melatonin on the quantity and quality of semen and testicular diameter in 8 Lori- Bakhtiari rams (4 control rams and 4 treatment rams) for 10 weeks during spring 2013. Each ram in the treatment group was received three tablets of 18 mg implantable melatonin subcutaneously at the base of ear after 5 weeks from the start of the trial. RESULTS: Semen parameters and testicular diameter measurements were evaluated on a weekly basis. No significant differences were observed between 2 groups before melatonin implanting. A significant difference was observed after melatonin implanting between 2 groups in the mean of testicular diameter (treatment: 33.5±0.25 and control: 30.67±0.07 cm), in semen volume (treatment: 1.34±0.03 and control: 0.87± 0.04 ml) and in sperm concentration (treatment: 1.76±0.05 and control: 1.37±0.04 109/ml) (p<0.05). However, other parameters including average of lives sperm percentage, total sperm motility, sperm progressive movement and percentage of abnormal sperm were not significant (p>0.05). CONCLUSIONS: The results of this experiment showed that the use of melatonin in the non-reproductive season resulted in increase of testicular size, semen volume and sperm concentration in Lori-Bakhtiari ram. However, the rest of the semen parameters have not been affected.

کلیدواژه‌ها [English]

  • Lori-Bakhtiari ram
  • melatonin
  • non breeding season
  • semen evaluation
  • testicular diameter
Abel, P., Axiak, V. (1990) Ecotoxicology and the Marine Environment. (1st ed.) Old University Building Press. Malta, Austria.

Adedeji, O., Adedeji, O., Adeyemo, O., Agbede, S. (2009) Acute Effects of Diazinon on Blood Paramters In The African Catfish (Clarias gariepinus). Int J Hematol. 5: 3940-3946.##

Adhikari, S., Sarkar, B., Chatterjee, A., Mahapatra, C. T., Ayyappan, S. (2004) Effects of cypermethrin and carbofuran on certain hematological parameters and prediction of their recovery in a freshwater teleost; Labeo rohita (Hamilton). Ecotoxicol Environ Saf. 58: 220-226.##

Atamanalp, M., Yanik, T. (2003) Alterations in hematological parameters of rainbow trout Oncorhynchus mykiss exposed to mancozeb. Turk J Vet Animal Sci. 27: 1213-1217.##

Aydin, R., Koprucu, K. (2005) Acute toxicity of diazinon on the common carp Cyprinus carpio embryos and larvae. Pestic Biochem Physiol. 82: 220-225. ##

Banaee, M., Mirvagefei, R., Rafei, G., Majazi Amiri, B. (2008) Effect of sub-lethal Diazinon  Concentrations on Blood Plasma Biochemistry. Int J Environ Res Public Health. 2: 189-198.##

Banaee, M., Sureda, A., Mirvagefei, R., Ahmadi, K. (2011) Effect of diazinon on biochemical parameters of  blood in rainbow trout (Oncorhynchus mykiss). Pestic Biochem Physiol. 99: 1-6.##

Blaxhall, P. C., Daisley, K. W. (1983) Roution haematological methods for use with fish blood. Fish Biol. 5: 771-781.##

Da Cuna, R., Vazquez, G., Piol, M., Guerrero, N., Maggese, M., Nostro, F. (2011) Assesment of the acute toxicity of the organochlorine pesticide endosulfan in Cichlasoma dimerus (Teleostei, Perciformes). Ecotoxicol Environ Saf. 74: 1065-1073.##

David, M., Mushigeri, S.B., Shivakumar, R., Philip, G.H. (2003) Responseof Cyprinus carpio (Linn) to sublethal concentration of cypermethrin: alterationsin protein metabolism profiles. Chemosphere. 56: 347-352.##

Dutta, H.M., Meijer, H.J.M. (2003) Sublethal effects of diazinon on the structure of the testis of bluegill, Lepomis macrochirus: a microscopic analysis. Environ Pollut. 125: 355-360.##

Evans, G.O. (2009) Animal Hematotoxicology. (2nd ed.) CRC Press. Bocarton, USA.##

Groff, J.M., Zinkl, J.G. (1999) Hematology and clinical chemistry of cyprinid fish Common carp and goldfish. Veterinary Clinics of North America: Exotic Animal Practice. 2: 741-776.##

Gross, R., Gliwitzki, M., Gross, P., Frank, K. (1996) Anaemia and haemo-globin status: A new concept and a new method of assessment. Food Nutr Bull. 17: 27-36. ##

Houston, A.H. (1990) Blood and circulation. In:  Methods in Fish Biology. Schreck, C.B., Moyle, P.B. (eds.). (1st ed.) American Fisheries Society. Bethesda, Maryland, USA.##

Inyang, I., Daka, E., Ogamba, E. (2010) Changes in electrolyte activities of Clarias gariepinus exposed to diazinon. Biol Environ Sci. 7: 198-200.  ##

Khoshbavar-Rostami, H.A., Soltani, M., Hassan, H.M.D. (2006) Immune response of great sturgeon (Huso huso) subjected to long-term exposure to sublethal concentration of the organophosphate, diazinon. Aquac Res. 256: 88-94. ##

Koprucu, S.S., Koprucu, K., Ural, M.S., Ispir, U., Pala, M. (2006) Acute toxicity of organophosphorous pesticide diazinon and its effects on behavior and some hematological parameters of fingerling European catfish (Silurus glanis L.). Pestic Biochem Physiol. 86: 99-105.##

Kumar, N., Antony, P., Pal, A.K., Remya, S., Aklakur, M., Rana, R.S., Gupta, S., Raman, R.P.,  Jadhao, S.B. (2011) Anti-oxidative and immuno-hematological status of Tilapia (Oreochromis mossambicus) during acute toxicity test of endosulfan. Pestic Biochem Physiol. 99: 45-52.##

Kumar, S., Sahu, N.P., Pal, A.K., Choudhury, D., Yengkokpam, S., Mukherjee, S.C. (2005) Effect of dietary carbohydrate on haematology, respiratory burst activity and histological changes in L. rohita juveniles. Fish Shellfish Immunol. 19: 331-344.##

Lermen, C.L., Lappe, R., Crestani, M., Vieira, V.P., Gioda, C.R., Schetinger, M.R.C., Baldisseretto, B., Moraes, G., Morsch, V.M. (2004) Effect of different temperature regimes on metabolic and blood parameters of silver catfish Rhamdia quelen. Aquac Res. 239: 497-507.##

Mikula, P., Modra, H., Nemethova, D., Groch, L., Svobodova, Z. (2008) Effects of subchronic exposure to LASSO MTX (alachlor 42% W/V) on hematological indices and histology of the common carp, Cyprinus carpio L. Bull Environ Contam Toxicol. 81: 475-479.##

Min, E.U., Kang, J.C. (2008) Effect of waterborne benomyl on the hematological and antioxidant parameters of the Nile tilapia, Oreochromis niloticus. Pestic Biochem Physiol. 92: 138-143.##

Nussey, G., Vanvuren, H.J., Dupreez, H.H. (1995) Effect of copper on the differential white blood cell counts of Mozambique tilapia Oreochromis mossambicus. Comp Biochem Physiol. 111: 381-388.##

Padash-Barmchi, Z., Safahieh, A.,  Bahmani, M.,  Savari, A., Kazemi, R. (2010) Immune responses and behavior alterations of Persian sturgeon fingerlings Acipenser persicus exposed to sublethal concentrations of diazinon. Toxicol Environ Chem. 92: 159-167.##

Pourgholam, R., Soltani, M., Hassan, M. D., Ghoroghi, A., Nahavandi, R., Pourgholam, H. (2006) Determination of diazinon LC50 in Grass carp Ctenopharyngodon idella and the effect of sublethal concentration of toxin on some hematological and biochemical indices. Iran J Fish Sci. 5: 67-82.##

Rehulka, J., Minark, B., Adamec, V., Rehulka, E. (2005) Investigation of physiological and pathological levels of total plasma protein in rainbow trout (Oncorhynchus mykiss). Aquac Res. 36: 22-32.##

Sakai, M. (1999) Current research status of fish immunostimulants. Aquac Res. 172: 63-92.##

Saravanan, M., Kumar, K.P., Ramesh, M. (2011) Haematological and biochemical responses of freshwater teleost fish Cyprinus carpio (Actinopterygii: Cypriniformes) during acute chronic sublethal exposure to lindane. Pestic Biochem Physiol. 100: 206-211. ##

Silveira-Coffigny, R., Prieto-Trujillo, A., Ascencio-Valle, F. (2004) Effects of different stressors in haematological variables in cultured Oreochromis aureus S. Comp Biochem Physiol  Part C: Toxicol Pharmacol. 139: 245-250.##

Stevens, M. L. (1997) Fundamentals of Clinical Hematology. (1st ed.) Saunders Company Press. Philadelphia , USA, 393 p.

Svobodova, M., Luscova, V., Drastichova, J., Habek, V. (2001) The effect of diazinon on haematological indics of common carp Cyprinus carpio. Acta Vet Brno. 70: 457-465.##

Svobodova, Z., Luscova, V., Drastichova, J., Svoboda, M., Zlabek, V. (2003) Effect of deltamethrin on haematological indices of common carp Cyprinus carpio. Acta Vet Brno. 72: 79-85.##

Tabarestani, M. (1985) Medical Hematology. (1st ed.) Mashhad University Press. Mashhad, Iran. 209 p.  ##

TRC, O.E.C.D. (1984) Guideline for testing if chemical section 2 ,on biotic systemmms. Paris, France. p. 180-202.##

Yekeen, T.A., Fawole, O.O. (2011) Toxic effects of endosulfan on haematological and biochemical indices of Clarias gariepinus. Afr J Biotechnol. 10: 14-19.#