تأثیر تغذیهای لاکتوکوکوس لاکتیس (Lactococcus lactis JF831150) بر وضعیت فلور باکتریایی روده تاسماهی ایرانی (Acipenser persicus) و مواجهه سازی با آئروموناس هیدروفیلا (Aeromonas hydrophila)

نویسندگان

1 بخش بهداشت و بیماریهای آبزیان موسسه تحقیقات بین المللی تاسماهیان دریای خزر، سازمان تحقیقات، آموزش و ترویج کشاورزی، رشت- ایران

2 گروه بهداشت و بیماریهای آبزیان دانشکده دامپزشکی دانشگاه تهران، تهران- ایران

3 موسسه تحقیقات علوم شیلاتی کشور، ایران

چکیده

زمینه مطالعه: لاکتوکوکوس لاکتیس یکی از باکتری‌های تولیدکننده اسید لاکتیک بوده و می‌تواند به عنوان یک پروبیوتیک احتمالی در تاسماهی ایرانی مورد توجه قرار گیرد. هدف: هدف از انجام تحقیق حاضر، مقاوم سازی تاسماهی ایرانی در برابر باکتری بیماریزای آئروموناس هیدروفیلا پس از تغذیه با باکتری Lactococcus lactis JF831150 بوده است. روش کار: در مطالعه حاضر، ابتدا 60 عدد تاسماهی ایرانی به مدت 56 روز با مقادیر متفاوت از L. lactis JF831150  تغذیه شدند و سپس فلور باکتریایی روده با استفاده از محیط‌های کشت TSA و MRS شمارش شدند در انتها میزان مقاومت آن‌ها در مواجه سازی با آئروموناس هیدروفیلا ارزیابی گردید. نتایج: استفاده از L. lactis JF831150  به مدت 56 روز در تغذیه تاس ماهیان بهبود معنی‌داری در کاهش فلور باکتریایی هترتروف بی هوازی اختیاری و افزایش باکتری‌های اسید لاکتیک در روده را  نشان داد. همچنین در مواجهه سازی تاسماهیان مورد تغذیه علیه باکتری آئروموناس هیدروفیلا مشخص گردید که در تیمار سوم (CFU/g 8 10) میزان بازماندگی بطور معنی‌داری نسبت به گروه شاهد و سایر تیمارها بیشتر بوده است. نتیجه گیری نهایی: نتایج این مطالعه نشان داد که مصرف L. lactis JF831150  به میزان 108 CFU در هر گرم غذا می‌تواند در بهبود فلور میکروبی روده تاسماهی ایرانی و افزایش مقاومت آن نسبت به بیماریزایی آئروموناس هیدروفیلا مؤثر باشد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of using Lactococcus lactis JF831150 on the status of the intestinal bacterial flora of Persian sturgeon (Acipenser persicus) and exposure to Aeromonas hydrophila

نویسندگان [English]

  • Alireza Shenavar Masouleh 1
  • Mehdi Soltani 2
  • Mohammad Reza Ahmadi 2
  • Mohammad Pourkazemi 3
  • Ali Taheri Mirghaed 2
1 Department of Aquatic Animal Health, International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht- Iran
2 Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Center of excellence of aquatic animal health, University of Tehran- Iran
3 Iranian Fisheries Science Research Instititue
چکیده [English]

BACKGROUND: Lactococcus lactis is one of the lactic acid bacteria that can be considered as a probiotic in Persian sturgeon. OBJECTIVES: The aim of the present study was to increase the resistance to Aeromonas hydrophila after feeding the L. lactis JF831150. METHODS: In the present study, first 60 fish were fed different doses of L.lactis JF831150 for 56 days. The bacterial flora were counted with  TSA and MRS. Resistance of fish against A. hydrophila were considered after feeding. RESULTS: The use of L. lactis JF831150 for 56 days in P. sturgeon showed significant improvement in decreasing heterotrophic aerobic bacteria flora and increasing the lactic acid bacteria in the intestine. In the face of fish against A. hydrophila in third treatment (108 CFU/g), the survival rate was shown to be significantly higher than the control and other treatments. CONCLUSIONS: The results showed that consumption of L. lactis JF831150 (108 CFU/g) could improve the intestinal flora and increase resistance to A. hydrophila, and therefore has  potential for use  with P. sturgeon.

کلیدواژه‌ها [English]

  • Acipenser persicus
  • Lactococcus lactis
  • Probiotic
  • Resistance
  • Survival

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