اثر تجویز خوراکی شیر گاوهای آبستن بر اسپرماتوژنز موشهای صحرایی نر

نویسندگان

1 گروه علوم پایه، دانشکده دامپزشکی دانشگاه تهران، تهران، ایران

2 گروه مامایی و بیماریهای تولید مثل، دانشکده دامپزشکی دانشگاه تهران، تهران، ایران

3 گروه پاتوبیولوژی، دانشکده دامپزشکی دانشگاه لرستان، خرم آباد، ایران

4 گروه پاتولوژی، دانشکده دامپزشکی دانشگاه تهران، تهران، ایران

چکیده

زمینه مطالعه: امروزه ناباروری یکی از مشکلات بزرگ جوامع بشری می‌باشد. هدف: بررسی اثرات تجویز خوراکی شیر مخلوط گاوداری و شیر گاوهای آبستن سنگین بر اسپرماتوژنز موش‌های صحرایی نر. روش کار: موش‌های گروه اول از روز 1 بارداری تا پایان شیردهی و سپس فرزندان نرآن‌ها تا بلوغ با شیر گاوهای آبستن سنگین گاواژ شدند. موش‌های گروه دوم از روز 12 بارداری تا 15 روز بعد از زایمان با شیر گاوهای آبستن سنگین گاواژ شدند.  موش‌های گروه سوم از روز 1 بارداری تا پایان شیردهی و سپس فرزندان نرآن‌ها تا بلوغ با شیر مخلوط گاوداری گاواژ شدند. موش‌های گروه چهارم از روز 12 بارداری تا 15 روز بعد از زایمان با شیر مخلوط گاوداری گاواژ شدند. موش‌های گروه شاهد در کل دوره پژوهش فقط با غذای مخصوص موش‌های صحرایی تغذیه شدند و در پایان توانایی زنده ماندن، انواع حرکت (پیشرونده، درجا و عدم تحرک) و شمار اسپرم‌ها و همچنین میزان تستوسترون خون موش‌ها بررسی گردید.  نتایج: تجویز هر دو نوع شیر تأثیری بر حرکات درجا و همچنین توانایی زنده ماندن اسپرم‌های گروه‌های آزمایشی نداشت  اما توانست سبب افزایش معنی‌دار عدم تحرک اسپرم‌ها و همچنین کاهش معنی‌دار شمار  اسپرم گروه‌های آزمایشی گردد همینطور میزان تستوسترون خون گروه‌های آزمایشی نسبت به گروه شاهد کاهش معنی‌دار یافت. نتیجهگیرینهایی: به طور کل مشخص گردید مصرف شیر گاوهای آبستن سنگین و یا شیر مخلوط گاوداری که دارای استروژن بالایی باشد می‌تواند سبب تغییر برخی از ویژگی‌های اسپرم که در باروری جنس نر دخیل‌اند گردند.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of oral administration of pregnant cow’s milk on male rat’s spermatogenesis

نویسندگان [English]

  • Zeynab Hamidiya 1
  • Parviz Tajik 2
  • morteza zendehdel 1
  • Omid Dezfoulian 3
  • Farhang Sasani 4
1 Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2 Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3 Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khoramabad, Iran
4 Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
چکیده [English]

BACKGROUND: Nowadays, infertility is one of the major problems of human societies. OBJECTIVES: To study oral administration of bulk milk and milk of late pregnant cows on spermatogenesis of male rats. METHODS: The first group of rats from day 1 of pregnancy until the end of lactation and then their male pups to maturity were treated with late pregnant cow’s milk. The second group from day 12 of pregnancy up to 15 days after delivery was treated with late pregnant cow’s milk. The third group of rats from day 1 of pregnancy until the end of lactation and then their male pups to maturity were treated with bulk milk. The fourth group from day 12 of pregnancy up to 15 days after delivery was treated with bulk milk. Rats in the control group during the study period were only fed with special food of rats and at the end viability, types of movement (progressive and in-place movement, immobility), number of sperms and also the serum testosterone level were elevated. RESULTS: Administration of both types of milk had no effect on in-place movement and also viability of sperms of experimental groups but they could cause a significant increase in sperm  immobility and a significant decrease in number of sperms of experimental groups. Also,the level of serum testosterone of experimental groups was significantly reduced in comparison with control group (p<0.05). CONCLUSIONS: Overall, it was determined consumption of late pregnant cow’s milk and bulk milk when it contains high estrogen can cause changes in some sperm species that are involved in male reproduction.

کلیدواژه‌ها [English]

  • Milk
  • estrogen
  • Spermatogenesis
  • infertility
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