اثرات لوامیزول بر پاسخهای ایمنی و مقاومت در برابر تنش تراکم در بچه ماهیان انگشت قد قزلآلای رنگین کمان (Oncorhynchus mykiss)

نویسندگان

1 گروه پاتوبیولوژی و کنترل کیفی آبزیان، دانشکده دامپزشکی و پژوهشکده آرتمیا و جانوران آبزی دانشگاه ارومیه، ارومیه، ایران

2 گروه میکروبیولوژی، دانشکده دامپزشکی دانشگاه ارومیه، ارومیه، ایران

3 گروه شیلات و آبزیان، دانشکده منابع طبیعی دانشگاه ارومیه، ارومیه، ایران

چکیده

زمینه مطالعه: تقویت سیستم ایمنی ماهیان در برابر تنش‌های موجود در مزارع پرورشی، امری ضروری به نظر می‌رسد. هدف: این تحقیق به منظور بررسی اثر لوامیزول در تقویت سیستم ایمنی قزل‌آلای رنگین کمان در برابر تنش تراکم انجام گرفت. روش کار: 1500 قطعه ماهی 50 گرمی قزل‌آلای رنگین کمان با تراکم m3/kg 33 در پنج تیمار (هر تیمار دارای سه تکرار) تقسیم گردید و  بترتیب با جیره‌های حاوی mg‌ 0 (گروه شاهد)، 100، 250 ، 500 و 1000 لوامیزول در هر کیلوگرم غذا به مدت 45 روز تغذیه شدند، سپس تا روز 60 همه تیمارها در شرایط تنش تراکم دو و سه برابر دوره پرورش قرار گرفتند. در روزهای 0، 15، 30، 45 و60 از تمام تیمارها نمونه سرم برای ارزیابی فاکتورهای فعالیت عامل مکمل، فعالیت لیزوزیم و میزان ایمونوگلوبولین تام تهیه گردید. نتایج: نتایج حاصل بیانگر این بود که تا پایان روز 45 تیمارهای دریافت کننده لوامیزول تنها از نظر فاکتور عامل مکمل سرم دارای تفاوت معنی‌دار (05/0>p) با گروه شاهد بودند. در پایان آزمایش در تنش تراکم دو و سه برابر، تیمار حاوی mg‌1000 لوامیزول در یک کیلوگرم غذا، افزایش معنی‌دار (05/0>p) فعالیت عامل مکمل، فعالیت لیزوزیم و ایمونوگلبولین تام سرم را نسبت به گروه شاهد نشان داد. بیشترین درصد بقا در پایان آزمایش در تیمار لوامیزول 1000 مشاهده گردید. نتیجه گیرینهایی: با توجه به نتایج این تحقیق، استفاده از لوامیزول به عنوان محرک ایمنی، حداکثر با غلظت 1/0% جیره غذایی موجب بالا بردن مقاومت ماهی قزل‌آلای رنگین کمان در برابر تنش تراکم زیاد و افزایش بقای آن می‌گردد.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of levamisole on immune responses and resistance against density stress in rainbow trout fingerling (Oncorhynchus mykiss)

نویسندگان [English]

  • Saeid Meshkini 1
  • Norouz Delirezh 2
  • Ali Akbar Tafi 3
1 Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine and Artemia and Aquatic Animals Institute, Urmia University, Urmia, Iran
2 Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Iran
3 Department of Fishery, Faculty of Natural Resources, Urmia University, Urmia, Iran
چکیده [English]

 
BACKGROUND: It is necessary to potentiate the immune system of fishes against stresses in farms. OBJECTIVES: This study was carried out to address the potential effect of Levamisole on immune system of rainbow trout against density stress. METHODS: 1500 fish (average weight of 50 g) were divided into 5 test groups, in which each test group was repeated three times with average density of 33 kg/m3. They were fed with commercial diet supplemented with Levamisole at concentrations of 0 (control), 100, 250, 500 and 1000 mg / kg for a period of 45 days. The fishes of all groups were then fed with Levamisole free diet and exposed to 2 and 3-fold density stress for the following 15 days. Blood samples were collected on days 0, 15, 30, 45 and 60 to evaluate the serum compliment and lysozyme activity as well as total immunoglobulins. RESULTS: The results showed that all used concentrations of Levamisole just had significant effect on compliment activity after 45 days feeding period (p<0.05). Higher levels of lysozyme and complement activity as well as total immunoglobulin were observed in 1000 mg/kg Levamisole treated group when exposed to density stresses 2 and 3-fold at the end of trial (day 60) (p<0.05). The highest overall survival was found in group which was treated with 1000 mg/kg of Levamisole. CONCLUSIONS: Our results revealed that using 0/1% Levamisole as an immunostimulator in commercial diet could potentiate rainbow trout against outbreak of high density stresses and increase its overall survival.

کلیدواژه‌ها [English]

  • Rainbow trout
  • levamisole
  • immune responses
  • density stress
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