بررسی اثر عصاره هیدروالکلی گیاه اسطوخدوس (Lavandula officinalis) براعمال حرکتی در موش صحرایی نر مدل شبه بیماری پارکینسون

نویسندگان

1 گروه زیست شناسی، دانشکده علوم پایه دانشگاه شهید چمران، اهواز

2 گروه فارماکولوژی، دانشکده داروسازی دانشگاه علوم پزشکی جندی شاپور، اهواز، ایران

3 گروه آمار، دانشکده ریاضی دانشگاه شهید چمران، اهواز، ایران

چکیده

زمینه مطالعه: بیماری پارکینسون یک اختلال ناشی از تخریب عصبی است که در حدود 1% از جمعیت بالای 50 سال اثر می‌گذارد. به طور کلاسیک پارکینسون به عنوان یک بیماری مربوط به سیستم حرکتی در نظر گرفته می‌شود و تشخیص آن بر مبنای گروهی از علائم حرکتی نظیر سفتی عضلات، کندی حرکت و اختلال در رفلکس‌های حرکتی و وضعی بنا نهاده می‌شود. هدف: هدف از این مطالعه ارزیابی اثرات عصاره هیدروالکلی گیاه اسطوخدوس بر اختلالات حرکتی موش‌های صحرایی مدل شبه بیماری پارکینسون است. روش کار: موش‌های صحرایی نر نژاد ویستار به چهار گروه تقسیم شدند (7=n). علائم بیماری پارکینسون قبل از تجویز عصاره اسطوخدوس به وسیله تزریق درون صفاقی (ip) رزرپین (mg/kg 5 ) ایجاد شد. سه گروه از موش‌های صحرایی مقادیر مختلف عصاره اسطوخدوس (mg/kg, po 400، 300، 200 ) و گروه شاهد نیز به مدت 6 روز و روزی دوبار به همان حجم آب مقطر دریافت کردند. در روز هفتم، سختی عضلات و دیگر علائم پارکینسون به وسیله بار تست، روتارود و میدان باز اندازه گرفته شد. نتایج: در این مطالعه بهبود علائم ناشی از بیماری پارکینسون با استفاده از عصاره هیدروالکلی اسطوخدوس مشاهده نشد و اختلالات حرکتی آن‌ها در مقایسه با گروه شاهد کاهش نیافت. نتیجه گیری نهایی: عصاره هیدروالکلی اسطوخدوس بر اختلالات حرکتی ناشی از بیماری پارکینسون بی تأثیر است.

کلیدواژه‌ها

عنوان مقاله [English]

Hydroalcoholic extract effect of Lavandula officinalis on motor functions in the male rat model of pseudo Parkinson’s disease

نویسندگان [English]

  • Maryam Abrishamdar 1
  • Ahmad Ali Moazedi 1
  • Ardeshir Arzi 2
  • Gholam Ali Parham 3
1 Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz, Ahvaz, Iran
2 Department of Pharmacology, Faculty of Pharmacology, Jondi Shapour University, Ahvaz, Ahvaz, Iran
3 Department of Statistics, Faculty of Mathematics, ShahidChamran University, Ahvaz, Iran
چکیده [English]

BACKGROUND: Parkinson’s disease (PD) is a neurodegenerative disorder that involves approximately 1% of the population older than 50 years. Classically, PD is considered a motor system disease, and its diagnosis is based on the presence of a set of cardinal motoric signs (e.g. rigidity, bradykinesia, motor and postural reflex disturbance). ObjectiveS: The present study aimed to evaluate the effects of hydro-alcoholic extract of lavender (Lavandula officinalis) on motor disorders of pseudo PD rats. Methods: Male Wistar rats were divided into four groups (n=7). PD signs were induced by intra-peritoneal (IP) injection of reserpine (5mg/kg) prior to administration of the lavender extract. Three goups of rats received different doses (200, 300, 400 mg/kg, P.O) of the lavender extract and sham group received distilled water at the same volume for six days and two times per day. At the seventh day, Catalepsy and  other signs of PD were measured in the bar test, rotarod and open field. Results: In this study, improvement in signs of Parkinson disease were not observed by applying lavender hydro-alcoholic extract and  motor disorders were not reduced in comparison with sham group. Conclusions: The lavender hydro-alcoholic extract does not have any effect on motor disorders of Parkinson disease.

کلیدواژه‌ها [English]

  • Parkinson
  • Lavandula officinalis
  • rotarod
  • bar test
  • open field

مراجع

Abilio, A.C., Silva, R.H., Carvalho, R.C., Grassel, C. (2004) Important role of striatal catalase in aging-and reserpine-induced oral dyskinesia, Neuropharmacology. 47: 263-272.
Aguiar, Jr.A.S., Araujo, A.L., De-Cunha, T.R., Speck, A.E., Ignacio, Z.M. (2009) Physical exercise improves motor and short-term social memory deficits in reserpinized rats, Brain res Bull. 79: 452-457.
Akhondzadeh, Sh., Kashani, L., Fotouhi, A., Jarvandi, S., Mobasheri, M. (2003) Comparison of Lavandula angastifolia Mill Tincture and imipramine in the treatment of mild to moderate depression, a double -blind randomized trial, Prog Neuropsychopharmacol Biol Psychiat. 27: 7-123.
Akhondzadeh, Sh., Maleki, J. (2006) Herbal medicines in the treatment of psychiatric and neurological disorders. Iran J Psychiat. 1: 1-11.
Brayfield, A. (2009) Martindale; The Complete drog Reference. (38th ed.) United Kingdom, london, Pharmaceutical press.
Buchbauer, G., Jirovetz, L., Jager, W. (1991) Aromatherapy evidence for sedative effects of the essential oil of lavender after inhalation, Z Naturforsch. 46: 167-172.
Burger, M., Fachinetto, R., Calegari, L. (2004) Effects of age on reserpine-induced orofacial and possible protection of diphenyl diselenide. Brain Res Bull. 64: 339-345.
Carvalho, R.C., Patti, C.C., Takatsu Coleman, A.A., Kameda, R.S., Souza, F.C. (2006) Effects of reserpine on the plus-maze discriminative avoidance task, Dissociation between memory and motor impairments, Brain Res. 1122: 179-183.
Cavanagh, H.M., Wilkinsin, J.M. (2002) Biological activities of lavender essential oil. Phytother Res. 16: 301-308.
Chang, S.Y. (2008) Effects of aroma hand massage on pain, state anxiety and depression in hospice patients with terminal cancer. Taehan Kanho Hakhoe Chi. 38: 493-502.
Colpaert, F.C. (1987) Pharmacological characteristics of tremor, rigidity and hypokinesia induced by reserpine in rat. Neuropharmacology. 26: 1431-1440
De moura linck, V., Lourence da silva, A., Figueiro, M. (2009) Inhaled linalool induced sedation in mice. Phytomedicine. 16: 303-307.
Di Matteo,V., Eposito, E. (2003) Biochemical and therapeutic effects of Alzheimer’s disease,parkinson’s disease. Curr Drug Target CNS Neurol. Disord. 2: 95-10.
Dubois, B., Pillon, B. (1997) Cognitive deficits in Parkinson’s disease. J Neurol. 244: 2-8.
Dutra, R.C.,  Andreazza, A.,  Andreatini, R.,  Tufik, S. (2002) Behavioral effects of MK-801 on reserpine-trearted mice, Prog Neuro-Psychoph. 26: 487-495.
Eric, P.M., Francis, C. (2002) 5-HT1A receotor activation and anti-cataleptic effects: high-efficacy agonists maximally inhibit haloperidol-induced catalepsy. Euro J Pharmacol. 453: 217-221.
Fathi Moghadam, H.,  Khodayar, M.J.,  Zarei Abarghouei, S.M.,  Shafiee Ardestani, M. (2010) Evaluation of the role of striatal cannabinoid CB1 receptors on movement activity of parkinsonian rats induced by reserpine, Saudi pharmaceut. 18: 207-215.
Ghotbeddin, Z., Moazedi, A.A., Parham, GH., (2007) Comparison the effect of different doses of zink supplementation on motor activity in young male rats. Iran J  Biol. 21: 543-548.
Hajhashemi, V., Ghannadi, A., Sharif, B. (2003) Anti- inflammatory and analgesic properties of the leaf extract and essential oil of Lavandula angastifolia Mill. J Ethnopharmacol. 89: 67-71.
Jahani Hashemi, H., Jafari, H., Abbasi, E., Momeni, A., Esmaeily, H. (2009) The effects of hydro alcoholic-extracts garlic on depression induced by reserpine in rats. Iran J Neurol. 7: 26- 27, 527-534.
Kim, H.M., Cho, SH. (1999) Lavender oil inhibits immediate-type allergic reaction in mice and rats. J Pharmacy Pharmacol. 51: 221-226.
Kim, Y., Kim, M., Kim, H., Kim, K. (2009) Effect of lavender oil on motor function and dopamine receptor expression in the olfactory bulb of mice. J Ethnopharmacol. 125: 31-35.
Kulkarni, S.K., Arzi, A., Kaul, P.N. (1980) Modification of drug-induced catatonia and tremors by quipazine in rats and mice, Japan. J Pharmacol. 30: 129-135.
Langeston, J.M. (2002) Parkinson’s disease, current and future challenges, Neurotoxicology. 23: 443-445.
Lewis, S.J., Dove, A., Robbins, T.W., Barker, R.A., Owen, A.W. (2003) Cognitive impairments  in realy Parkinson’s disease are accompanied by reductions  in activity in frontostriatal neural circuitry. J Neurosci. 23: 6351-6356.
Mehrabani, M., Modirian, E., Ebrahimabadi, A.R. (2007) Study  of  the  Effects  of  Hydro-methanol  Extracts  of Lavandula  vera DC.  and  Cuscuta Epithymum Murr. on the Seizure Induced by Pentylentetranzol in Mice. U Kerman J. 14: 25-32.
Mirzaei F, Keshtgar S, Kaviani M,(2009).  The effect of Lavender essence smelling during labor on cortisol and serotonin plasma levels and anxiety reduction in nulliparous women, U Kerman J. 16: 245-254.
Nigel, W., Bond, Eros, L. (1976) Effects of prenatal alcohol consumption on open- field behavior and alcohol preference in rats. Psychopharmacology. 43: 163-165.
Nikfarjam, M., Parvin, N. (2010) The effect of  Lavandula angustifolia in the treatment of mild to moderate depression. U Shahrekord J. 2: 66-74.
Prinssen, P.M.E., Colpaert, C.F., Koek, W. (2002) 5-HT1A receptor activation and anti-efficacy agonists maximally inhibit haloperidol-induced catalepsy. Euro J Pharmacol. 453: 217-221.
Rabiei, Z., Rafieian, M., Mokhtari, SH. (2013) The effect of pretreatment with different doses of Lavandula Officinalis ethanolic extract on memory, learning and nociception. Biomed Pathol. 4: 71-76.
Re, L., Barocci, S.,  Sonnino, S.,  Mencarelli, A.,  Vivani, C. (2000) Linalool modifies the nicotinic receptor-ion channel kinetics at the mouse neuromuscular junction, Pharmacol Res. 42: 82-177.
Rezaei, A., Jafari, B., Jalilzadeh, H.M. (2010) Study of sedative, preanaesthetic and anxiolytic effect of herbal extract of Lavandula Stoechas in comparison with diazepam. U Tabriz J. 3: 899-905.
Salah, S.M., Jager, A.K. (2005) Two flavonoids from Artemisia herba-alba asso with in vitro GABA A-benzodiazepine receptor activity. J Ethnopharmacol. 99: 145-146.
Shaw, D., Annett, J.M., Doherty, B., Leslie, J.C. (2007) Anxiolytic effects of lavender oil inhalation on open-field behaviour in rats, Phytomedicine. 14: 613-20.
Sikiric, P., Marovic, A., Matoz, W., Anic, T.,  Buljat, G. (1999) A behavioural study of The effect of pentadecapeptide BPC 157 in Parkinson’s disease models in mice and gastric lesions induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. J Physiol. 93: 505-512.
Walsh, E., Wilson, C. (1999) Complementary therapies in long stay neurology in-patients settings. Nurs stand. 13: 32-35.

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