بررسی اثر ادجوانی نانوکیتوزان بر ایمنی زایی واکسن کشته‌ی آئروموناس هیدروفیلا در ماهی کپور معمولی(Cyprinus carpio)

نوع مقاله: میکروبشناسی و ایمنی شناسی


1 گروه علوم درمانگاهی، دانشکده دامپزشکی دانشگاه شهید چمران اهواز، اهواز ، ایران

2 دانش آموخته دامپزشکی، دانشکده دامپزشکی دانشگاه شهید چمران اهواز، اهواز، ایران

3 گروه پاتوبیولوژی، دانشکده دامپزشکی دانشگاه شهید چمران اهواز، اهواز، ایران


زمینه مطالعه: اخیرا گزارشات متعددی از اثرات تحریک ایمنی و ادجوانی نانوکیتوزان در حیوانات مختلف گزارش شده است. هدف: در این مطالعه، اثر ادجوانی نانوکیتوزان تولید شده از کیتوزان حاصل از کیتوزان پوسته میگو بر ایمنی زایی واکسن کشته آئروموناس هیدروفیلا در ماهی کپور معمولی (Cyprinus carpio) مورد بررسی قرار گرفت. روش کار: تعداد 300 عدد ماهی کپور معمولی (با وزن متوسط 6/5±51 گرم) به چهار گروه (هر گروه 75 ماهی و 25 ماهی در هر تکرار) تقسیم شدند. گروه 1، 2 و 3 با واکسن آئروموناس هیدروفیلا ایمن شدند. در گروه 1 واکسن بدون ادجوان، گروه 2، واکسن همراه ادجوان فروند و گروه 3 واکسن همراه ادجوان نانوکیتوزان استفاده شد. گروه 4 (شاهد) با سرم فیزیولوژی تزریق گردید. ماهی‌های هر گروه در روز صفر و 14 ایمن شدند و به مدت 6 هفته با شرایط مشابه نگهداری و تغذیه شدند. خونگیری در انتهای هفته 2، هفته 4 و هفته 6 تحقیق انجام گرفت. برخی شاخص‌های ایمنی مانند تعداد گلبول‌های سفید خونی، فعالیت لایزوزیم و قدرت ضدباکتریایی سرم، فعالیت کمپلمان، میزان احیای NBT ، پروتئین و گلوبولین تام و عیار آنتی‌بادی ضد آئروموناس هیدروفیلا بررسی شد. در انتهای دوره ماهی‌ها با غلظت LD50 باکتری زنده آئروموناس هیدروفیلا چالش داده شدند و میزان تلفات طی 10 روز ثبت شد. نتایج نشان داد که تعداد گلبول‌های سفید خونی، فعالیت لایزوزیم سرم، میزان گلوبولین، میزان فعالیت NBT و عیار پادتن در دو تیمار فروند و نانوکیتوزان در مراحل نمونه گیری افزایش معنی داری نسبت به تیمار کنترل یافت(P<0.05). در میزان فعالیت ضدباکتریایی و سطح آلبومین سرم در تیمارهای مختلف تفاوت معنی داری مشاهده نگردید(P>0.05). تلفات بعد از چالش نیز در هر دو تیمار نانوکیتوزان و فروند کاهش معنی داری نسبت به تیمار بدون ادجوان و کنترل نشان دادند(P<0.05). نتیجه‌گیری نهایی: بر اساس نتایج این تحقیق نانوکیتوزان دارای خواص ادجوانی مناسب و قابل رقابت با ادجوان فروند بوده و گزینه خوبی برای جایگزینی ادجوان های رایج ماهی می باشد.


عنوان مقاله [English]

Adjuvant effects of nanochitosan on immunogenicity of Aeromonas hydrophila vaccine in Cyprinus carpio

نویسندگان [English]

  • Mojtaba Alishahi 1
  • Ozra Hajipoor 2
  • Masood Ghorbanpoor 3
  • Mehrzad Mesbah 1
1 1Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 2Student of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 3Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz-Iran
چکیده [English]

BACKGROUND: Recently chitosan nanoparticles have been applied as an immunostimulant and adjuvant in biomedical fields. OBJECTIVES: In this study the adjuvant effects of nanochitosan, derived from shrimp shell's chithin, on immunogenicity of Aeromonas hydrophila vaccine in common carp were evaluated. METHODS: Totally 300 juvenile common carp (51±5.6 g, Mean ±SD) were randomly divided into four groups each contained three subgroups with 25 fishes. Groups 1, 2 and 3 were immunized intraperitoneally with A. hydrophila vaccine. No adjuvant used in group 1, but nanochitosan and freund adjuvant were used with vaccine in groups 2 and 3 respectively. Group 4 (Control) injected with normal saline instead of vaccine. Fish were immunised at days zero and 14 and fishes were reared in the same situation for 6 weeks. Serum samples were taken every other weeks and immunological parameters including WBC, serum lysozyme and bactericidal activity, serum complement activity, NBT reduction activity, total protein and globulin levels and anti A.hydrophila antibody titer were compared among the groups. At the end of experiment all groups were challenged with LD50 concentration of A. hydrophila and mortality recorded for ten days. RESULTS: WBC, serum lysozyme and complement activity, NBT activity, globulin level and anti A.hydrophila antibody titer were increased in nanochitosan and freund groups compare to control group (P<0.05). Serum bactericidal activity and serum albumin level showed no significant change among the groups (P>0.05). Challenge mortality decreased in immunised groups compare to control. CONCLUSIONS: Nonochitosan posses proper adjuvant effects which is comparable with freund adjuvant and can be a good candidate as an alternative to current adjuvant in fish vaccines.

کلیدواژه‌ها [English]

  • Nanochitosan
  • Adjuvant
  • Cyprinus carpio
  • Aeromonas hydrophila
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