بررسی پروتئین های سرم طیور بومی بوسیله الکتروفورز

In this study variations in the protein levels of sera of 200 native fowl were investigated according to age «<4, 4-5.5, 5.5-7.5, 7.5-15, > 15 months) and laying.
Cellulose acetate electrophoresis revealed six (albumin, alpha -1, alpha -2, beta -1, beta-2 and gammaglobulins) or four (albumin and alpha-, beta- and gamma globulins) separat serum protein bands.
Four separat proteins (albumin and alpha-, beta- and gammaglobulins) Were separated in agarose gel electrophoresis. The amounts of proteins of sera in separate age groups were significantly different. Positive and significant correlations were observed between age and total protein (g/dl; r=0.724), albumin (g/dl; r=0.387), total globulin (both% of total protein and g/dl; r=0.646 and r=0.763, respectively), alpha-l globulin(g/dl r=O.555), alpha-2globulin(g/dl;r=:0.560), beta-1 globulin (g/dl; r=0.402), beta -2 globulin (g/dl; r=0.485), gamma - globulin (both % of total protein and g/dl; r=O.678 and r=0.791, respectively).
In contrast negatiive and significant correlations were found between the age and albumin (% of total protein; r=-0.646), beta -2globulin (g/dl r=-0.259)md A/G (r=-0.643).
Laying had a significant effect on the amounts of serum proteins. Positive and significant correlations were observed between the laying and
total protein (g/dl), albumin (g/dl), total globulin (both % of total protein and g/dl, respectively), alpha -1 globulin (g/dl), alpha -2-globulin (g/dl), beta -1-globulin (g/dl), beta-2-globulin (g/dl), gamma-globulin (both % of total protein and g/dl, respectively).
Negative and significant correlation were found between the laying and albumin (% of total protein), beta -1-globulin (% of total protein), beta -2-globulin (% of total protein) and A/G. Significant correlations between various serum protein s were also observed.