تلقیح مصنوعی داخل رحمی به روش لاپاروسکوپی و تلقیح سرویکال در میشهای سوپر اووله شده در برنامه انتقال جنین

In order to study efficacy of cervical Al (once and twice
inseminations) and laparoscopic intrauterine insemination, in egg
recovery rate and fertilization rate, three experiments were done.
In each of the 1st and 2nd experiments, 5 moghani ewes were
inseminated in the external oss of cervix. Ewes had been
synchronized by vaginal sponges and super ovulated by injections of
800 or 1200 IU of eCG. Insemination was done at 35-40 hours
after sponge removal (the times of experiments and the ewes were
different). In the first experiment, every ewe was inseminsted with
0.2 to 0.3 ml of semen diluted by skimed milk and in the secon
one, every ewe was inseminated with the same volume of semen
diluted by tris buffer each contained 200-300 >406 live motile
sperm cells. The maximum interval between obtaining semen and
insemination was 3 hours. In the first experiment, every ewe was
injected 100 IU eCG immidiately after insemination. In the third
experiment 8 Ghezel X Ciucy ewes were synchronized by PGF2a
and super ovulated by FSH, and divided into two groups.
Bothgroups were inseminated by a semen diluted by tris-buffer and
contained 100x 106 sperm cells/nil. The first group were
inseminated cervicaly with 0.3 ml of diluted semen twice, at 42 and
48 h after the last injection of FSH considering heat detection. In
the 2nd group, laparoscopic intrauterine insemination was
performed by 0.1 ml of diluted semen into each uterine horn at
44-45 h after the last injection of FSH. In bouth groups, 60 micro
gram of GnRH was injected immediately after insemination (in the
first group after the first insemination). Laparotomy was
performed on all of the ewes 6 day after insemination, and uterine
horns were flushed for recovery of the embryos. In the first
experiment, 13 eggs were recovered from 23 ovulations (recovery
rate=57%), of the 7 were fertilized (54%). In the 2nd experiment,
11 eggs were recovered in 35 ovulations (31% recovery rate) and
even one egg was not fertilized (0% fertilization rate). In the first
group among the 3rd experiment (twice cervical inseminations), 23
eggs were recovered from 41 ovulations (58% recovery rate) in
which 14 were fertilized (65% fertilization rate) and 10 were
suitable for embryo transfer. In the 2nd group of this experiment
(laparoscopic insemination), 18 eggs were recovered from 23
ovulations (78% recovery rate) and all were fertilized (100%) in
which 11 were suitable for embryo transfer. In conclusion these
experiments indicate that cervical insemination may not be a
suitable method in super ovulated ewes. In contrast, intrauterine
insemination by laparoscopy is an effective method and even in
comparision with twice cervical inseminations, it is significantly
more effective. Correlation rate (Chooprov index) between
insemination method and fertilization rate in the 3rd experiment
was 47%. .