نویسندگان
1 گروه میکروبیولوژی، پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری
2 موسسه تحقیقات واکسن و سرم سازی رازی کرج
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
In this study a nested-PCR assay was optimized for detection of two BVDV biotype of NADL strain. A part of 5' non-coding region of virus, 249 bp in size, was amplified in RT-PCR. PCR product was cloned in a pTZ57R/T vector and sequencing results confirmed the specificity of the test. Internal primers were designed and a 155 bp DNA fragment was amplified in nested-PCR. The sensitivity of RT-PCR and nested-PCR for detection of virus in cell culture were found to be 104 TCID50 and 102 TCID50, respectively. Seven cell cultures were tested for BVDV contamination using ELISA, RT-PCR and nested-PCR. Results indicate that sensitivity of molecular tests for detection of virus in cell culture samples is higher than ELISA.
کلیدواژهها [English]
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