مطالعه برخی پاسخهای ایمنی ماهی قزل آلای رنگین کمان (Onchorhyncus mykiss) به برخی انتی ژنهای استرپتوکوکوس اینیایی

نویسندگان

1 گروه بهداشت و بیماریهای آبزیان دانشکده دامپزشکی دانشگاه تهران

2 گروه علوم درمانگاهی دانشکده دامپزشکی دانشگاه تهران

3 گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران

چکیده

Some specific and non-specific immune variables of
rainbow trout were assessed following vaccination of fish
with formalin killed cells (FKC) and FKC containing extra
cellular products (ECP) of S. iniae. Rainbow trout
weighing 80-100 g were vaccinated by intraperitoneal
(i.p), dip and oral routes using FKC and FKC plus ECP
with or without Fround adjuvant (FA) at 16-17°C.
Antibody titration, lysozyme activity, serum bacterial
killing activity and population of immunocompetent cells
were measured on 3, 6, 9, 12, 15 and 18 weeks
postvaccination. Data were analyzed by ANOVA. Results
showed that the highest antibody titers were produced in i.p
vaccinated fish with FKC plus ECP and immunized fish
with FKC by dip, respectively. However, oral
administration resulted in the lowest response. Also, the
level of antibody production was higher during initial
period of post-immunization, while it reduced to lower
levels at the end of sampling time. Similar results were
obtained when lysozyme activity and bacterial killing
capacity of sera samples were estimated. Moreover, while,
leukocyte and lymphocyte populations in immunized fish
were generally higher than control groups, heterophil and
monocyte counts were varied during the sampling periods.
Reuslts show that both humeral and cellular immunities of
trout are enhanced following immunization of fish with
FKC with or without ECP administered as i.p and dip.
However, i.p administration of FKC with or without ECP
could cause higher response than both dip and oral routes.

کلیدواژه‌ها


عنوان مقاله [English]

STUDY ON SOME IMMUNOLOGICAL RESPONSES OF RAINBOW TROUT (ONCHORHYNCHUS MYKISS) TO SOME ANTIGENS OF STREPTOCOCCUS INIAE

نویسندگان [English]

  • Mahdi Soltani 1
  • Mojtaba Alishahi 1
  • Parvaneh Khazraeenia 2
  • Mohammad Rabani 3
  • Amir Sattari 1
چکیده [English]

Some specific and non-specific immune variables of rainbow trout were assessed following vaccination of fish with formalin killed cells (FKC) and FKC containing extra cellular products (ECP) of S. iniae. Rainbow trout weighing 80-100 g were vaccinated by intraperitoneal (i.p), dip and oral routes using FKC and FKC plus ECP
with or without Fround adjuvant (FA) at 16-17°C.Antibody titration, lysozyme activity, serum bacterial killing activity and population of immunocompetent cells
were measured on 3, 6, 9, 12, 15 and 18 weeks postvaccination. Data were analyzed by ANOVA. Results showed that the highest antibody titers were produced in i.p vaccinated fish with FKC plus ECP and immunized fish with FKC by dip, respectively. However, oral administration resulted in the lowest response. Also, the
level of antibody production was higher during initial period of post-immunization, while it reduced to lower levels at the end of sampling time. Similar results were
obtained when lysozyme activity and bacterial killing capacity of sera samples were estimated. Moreover, while,leukocyte and lymphocyte populations in immunized fish were generally higher than control groups, heterophil and monocyte counts were varied during the sampling periods.Reuslts show that both humeral and cellular immunities of trout are enhanced following immunization of fish with FKC with or without ECP administered as i.p and dip.However, i.p administration of FKC with or without ECP could cause higher response than both dip and oral routes.

کلیدواژه‌ها [English]

  • immune response
  • Immunization
  • Rainbow trout
  • Streptococcus iniae