مطالعه پادگن های دخیل در ایمنی همورال ویروس IBR

نویسندگان

1 گروه میکروبیولوژی و ایمونولوژی، دانشکده دامپزشکی دانشگاه تهران

2 دانش آموخته دکتری دامپزشکی دانشکده دامپزشکی دانشگاه تهران

چکیده

The objective of this study was to determine the protein
pattern and antigenic structure of BHV-1. Ten viral isolates
from cows with clinical signs of respiratory infections were
selected for this study and confirmed by SN test. All
samples were cultured on cell cultures and purified by
ultracentrifugation. Purified isolates were electerophorsed
using SDS-PAGE method and immunoblotted. Viral
antigens were detected using anti BHV-1 bovine antiserum
and molecular weight of the bands were determined. All
samples showed identical band number and molecular
weight except for one sample which revealed an additional
120-125 KDa band. Also immunoblotting data resulted in
identified of twelve bands (150, 130, 115, 97, 77, 70, 55,
45, 40, 38, 32 and 25 KDa) suggesting involvement of
these antigens in evoking humoral immune response in
affected cattle.

کلیدواژه‌ها


عنوان مقاله [English]

STUDY OF EFFECTOR ANTIGENS OF IBR VIRUS ON HUMORALIMMUNITY

نویسندگان [English]

  • Farhid Hemmatzadeh 1
  • Hadi Keyvanfar 1
  • Mahdi Ahmadi 2
1
2
چکیده [English]

The objective of this study was to determine the protein pattern and antigenic structure of BHV-1. Ten viral isolates from cows with clinical signs of respiratory infections were selected for this study and confirmed by SN test. All samples were cultured on cell cultures and purified by ultracentrifugation. Purified isolates were electerophorsed using SDS-PAGE method and immunoblotted. Viral antigens were detected using anti BHV-1 bovine antiserum and molecular weight of the bands were determined. All samples showed identical band number and molecular weight except for one sample which revealed an additional 120-125 KDa band. Also immunoblotting data resulted in identified of twelve bands (150, 130, 115, 97, 77, 70, 55,45, 40, 38, 32 and 25 KDa) suggesting involvement of these antigens in evoking humoral immune response in affected cattle.

کلیدواژه‌ها [English]

  • antigen
  • IBR
  • immunoblotting
  • SDS-PAGE
  • virus