آنالیز ملکولی سه سویه متعلق به سروتیپB/793 ویروس های برونشیت عفونی طیور جدا شده از مرغداری های صنعتی ایران

نویسندگان

1 گروه علوم درمانگاهی، دانشکده دامپزشکی دانشگاه تهران

2 دانشگاه علوم پزشکی ایران

چکیده

Infectious bronchitis (IB) disease is one of the important
respiratory diseases of poultry that causes annually large
economic losses in poultry industry of Iran. The aim of this
study is molecular characterization of S1 gene of Iranian
infectious bronchitis viruse (IBV) belonged to 793/B
serotype. The whole S1 gene of three local IBV strains in
RT-PCR, showed a band above 1600 base pair (bp) in gel
electrophoresis. RFLP analysis using 3 restriction
enzymes, HaeIII, HindIII and EcorI, showed 793/B
serotype pattern. The S1 gene of these strains were
sequenced and compared with 30 reference IBV strains.
Identity Plot (IP) of nucleic acids and amino acids
sequences were also designed. Moreover, nucleic acids
differences in 1659 bp of S1 gene were calculated by
distance method: nucleotide: Kimura 2-parameter and
finally, the phylogenetic tree of S1 gene sequence strains
with the highest validity in branching were designed. Three
Iranian strains belonged to 793/B genotype with nucleotide
differences of 5.64-6.07 % to UK/793/B as a prototype of
793/B and 26.02-26.16 % to H120 as a vaccinal strain.
Regarding to low homology and weak cross protection
between 793/B serotype and Massachusetts vaccinal strain,
it would be a possible cause for failure in vaccination and
outbreak of IB disease in vaccinated flock of poultry
industry.

کلیدواژه‌ها

عنوان مقاله [English]

MOLECULAR ANALYSIS OF THREE IRANIAN ISOLATES BELONGED TO 793/B SEROTYPE OF INFECTIOUS BRONCHITIS VIRUSES

نویسندگان [English]

  • mehdi vasfimarandi 1
  • Mahdi Vasfi Marandi 1
  • Hossein Keyvani Aminae 2
چکیده [English]

Infectious bronchitis (IB) disease is one of the important respiratory diseases of poultry that causes annually large economic losses in poultry industry of Iran. The aim of this study is molecular characterization of S1 gene of Iranian infectious bronchitis viruse (IBV) belonged to 793/B serotype. The whole S1 gene of three local IBV strains in RT-PCR, showed a band above 1600 base pair (bp) in gel electrophoresis. RFLP analysis using 3 restriction enzymes, HaeIII, HindIII and EcorI, showed 793/B serotype pattern. The S1 gene of these strains were sequenced and compared with 30 reference IBV strains.Identity Plot (IP) of nucleic acids and amino acids sequences were also designed. Moreover, nucleic acids differences in 1659 bp of S1 gene were calculated by distance method: nucleotide: Kimura 2-parameter and finally, the phylogenetic tree of S1 gene sequence strains with the highest validity in branching were designed. Three Iranian strains belonged to 793/B genotype with nucleotide differences of 5.64-6.07 % to UK/793/B as a prototype of 793/B and 26.02-26.16 % to H120 as a vaccinal strain.Regarding to low homology and weak cross protection between 793/B serotype and Massachusetts vaccinal strain,it would be a possible cause for failure in vaccination and
outbreak of IB disease in vaccinated flock of poultry industry.

کلیدواژه‌ها [English]

  • 793/B serotype
  • IBV
  • RT-PCR/RFLP
  • sequencing
مجله تحقیقات دامپزشکی (Journal of Veterinary Research)
دوره 62، شماره 1
فروردین 1386
صفحه 69-80

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