مجله تحقیقات دامپزشکی (Journal of Veterinary Research)

مجله تحقیقات دامپزشکی (Journal of Veterinary Research)

بررسی آلودگی به ویروس IHN در مزارع پرورش ماهی قزل آلای رنگین کمان (On corhynchus mykiss) در دو استان تهران و چهار محال و بختیاری با استفاده از تکنیک آنتی بادی درخشان به روش غیر مستقیم

نویسندگان
روزبه فلاحی 1
مهدی سلطانی 2
سید جلیل ذریه زهرا 3
فرهید همت زاده 4
1 موسسه تحقیقات واکسن و سرم سازی رازی حصارک
2 گروه بهداشت و بیماریهای آبزیان، دانشکده دامپزشکی، دانشگاه تهران
3 موسسه تحقیقات شیلات ایران
4 گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران
چکیده
Objective: Serological diagnosis of infectious haematopoietic necrosis virus (IHNV) in tissues of kidney, spleen and liver of rainbow trofi(O.mykiss)fry. Design: Cross- sectional study. Animals:Ninty nine samples consisting of smears from kidney, spleen and liver were obtained from rainbow trout. Procedure: Kidney, spleen and liver from rainbow trout (O.mykiss) fry less than 3 g body weight obtained from some farmed rainbow trout of Tehran and Char mahal - va- Bakhtiari provinces. Indirect immunofluorescent antibody test (IFAT) was undertaken using both polyclonal and monoclonal antibodies to IHNV to identify the IHNV antigens in the tissue samples. Results: Only eleven samples from the region of Char mahal - va - Bakhtiari were positive for IHNV using both monoclonal and polyclonal antibodies to IHNV.Conclusion: Because of economic losses due to disease caused by IHNV in farmed rainbow trout , it is necessary to do a comprehensive screening test, virus isolation and evaluation of the pathogenicity of recovered virus.

عنوان مقاله English

Serological Diagnosis of Infectious Haematopoietic Necrosis Disease (IHN) in Rainbow Trout (Oncorhynchus mykiss) using Indirect Fluorescent Antibody Test

نویسندگان English

Roozbeh Fallahi 1
Mahdi Soltani 2
Seyed Jalil Zorriehzahra 3
Farhid Hemmatzadeh 4
1 Unit of Aquatic Animal Health and Diseases, RaTi Vaccine and SerumRe search Institute, Karaj - I ran
چکیده English

Objective: Serological diagnosis of infectious haematopoietic necrosis virus (IHNV) in tissues of kidney, spleen and liver of rainbow trofi(O.mykiss)fry. Design: Cross- sectional study. Animals:Ninty nine samples consisting of smears from kidney, spleen and liver were obtained from rainbow trout. Procedure: Kidney, spleen and liver from rainbow trout (O.mykiss) fry less than 3 g body weight obtained from some farmed rainbow trout of Tehran and Char mahal - va- Bakhtiari provinces. Indirect immunofluorescent antibody test (IFAT) was undertaken using both polyclonal and monoclonal antibodies to IHNV to identify the IHNV antigens in the tissue samples. Results: Only eleven samples from the region of Char mahal - va - Bakhtiari were positive for IHNV using both monoclonal and polyclonal antibodies to IHNV.Conclusion: Because of economic losses due to disease caused by IHNV in farmed rainbow trout , it is necessary to do a comprehensive screening test, virus isolation and evaluation of the pathogenicity of recovered virus.

کلیدواژه‌ها English

IFAI
IHNV
Rainbow trout
مجله تحقیقات دامپزشکی (Journal of Veterinary Research)
دوره 61، شماره 1 - شماره پیاپی 822096
242 مجله تحقیقات دامپزشکی
بهار 1385
صفحه 19-22