شناسایی دگرگونی ژنتیکی در ویروس تب برفکی تیپ A با استفاده از تعیین ردیف نوکلئوتیدی قسمتی از ژن VP1 ویروس

نویسندگان

1 آموزشکده دامپزشکی، دانشگاه بوعلی سینا، همدان

2 پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری، تهران

3 مرکز فدرال تحقیقات بیماریهای ویروسی حیوانات، توبینگن

4 گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه تهران

چکیده

,1s1ya:.J'$n :7tb
!9y.tu;1 9; q 5t'1& p I r lt 4!lr 9 61,; .l.iy c.!! ob lr+ : tt 4jr.'j
.Al JLrob.;qrolr,J'fll t q
..rlaLr. 4 RT-PCR ri,Sle 1r o.ri 6bi!l Jt-t5 RI\A :.pe,
.pe1 *q&,.5b.'.;9; VPr Oijl ,r,iiqo-f .ddisl-t,r-a.ttJ
nlceloltl Jlhr""gbyp,glrrsc*l . d,ri$ multiplexRT-PCR
cycle sequencing cie) jlosu:pl t r PCR Jr-e .ri
. 'r-JJl9; a{.Jt Fluorescent dye deoxy-terminator
ar.bgy.5bai9rjra . rj!9.tc",.i.ssiy;.1.E;;l Cl 1 t'bzti9.'j JSil :CJ-F
03; ; I o* 6ta,ltr- o*, ri sgA 3; ; I 9r p a5 6tQjrl ; I 6rf 19 51
olr*;A
"t.$9-
a;9T6tr.qtol Jlf .5borl,: a*,Jr.L ..uri,rrJrl.'d
. rb gtiu l.z.glnLr)L.!G
strrl:4 o5.r-r.r.r"rl! o a*ttL" a1l ,Jli4;i! C 4?9j tl :,,sfa++-;
6bl.iir .5;tr.,.:; 9 Jtk.i,fcpot^:at";ia;Jl . dl.t criJSJS.i.ff i
ol5,i;1.: *5-i;*.1: o.rftl: alx. . o-rl p;Y 4t ,g!1 ,lt'r.S) qiti dlP
.AV 21,\ 9L.i,2\ oze:,\YAd,;!4;
"t.$9-
a;9T6tr.qtol Jlf .5borl,: a*,Jr.L ..uri,rrJrl.'d
. rb gtiu l.z.glnLr)L.!G
strrl:4 o5.r-r.r.r"rl! o a*ttL" a1l ,Jli4;i! C 4?9j tl :,,sfa++-;
6bl.iir .5;tr.,.:; 9 Jtk.i,fcpot^:at";ia;Jl . dl.t criJSJS.i.ff i
ol5,i;1.: *5-i;*.1: o.rftl: alx. . o-rl p;Y 4t ,g!1 ,lt'r.S) qiti dlP

عنوان مقاله [English]

Detection of a Genetic Variation Within Foot-and- Mouth Disease Virus Type A Using Sequencing Analysis of VP1 Gene

نویسندگان [English]

  • Ali Asghar Bahari 1
  • Seyed Ali Ghorshi 2
  • Atfrid Marquardt 3
  • Taghi Taghipour Bazargani 4
1
2
3
4
چکیده [English]

Objective: Study on genetic variation of foot-and-mouth disease (FMD) virus, the causetive agent of FMD outbreaks in Iran. Design: Case study. Animals: The tongue and mouth epithelial lesions of 11 clinical livestock suspected to FMD from 9 outbreaks during S eptember, 2002. Procedures: Total extracted RNAs were used in diagnostic one step RT-PCR. VP1 gene from positive samples was then amplified in the multiplex RT-PCR for current types viral isolates in Iran. The PCR products were sequenced using fluorescent dye deoxy-terminator cycle sequencing. Results: Nine of the era mined samples out of whole were positive for FMD viral genome. Except the samples of type A, which both samples were from an outbreak in Isfahan, all of the positive samples were type O. The Considerable variation revealed in amino acids sequences of type A sample. Conclusion: Results of this study shows evolution of a new genetic variant. However, serological and crossneutralization assays are required for confirming the antigenic diversity.

کلیدواژه‌ها [English]

  • foot-and-mouth disease virus (FMDV)
  • Genetic variation
  • RTPCR
  • sequencing analysis
  • VP1