اثرات محافظتی بر استخوان متعاقب تجویز عصاره الکلی گیاه گلدر (Otostegia persica) در موش‌های دیابتی شده توسط استرپتوزوتوسین: مطالعه هیستومورفومتری

نویسندگان

1 گروه علوم پایه دانشکده دامپزشکی دانشگاه تهران، تهران–ایران

2 گروه فارماکولوژی دانشکده دامپزشکی دانشگاه شیراز، شیراز–ایران

3 گروه فارماکولوژی دانشکده دامپزشکی دانشگاه تهران، تهران–ایران

چکیده

زمینه مطالعه: ‌داروهای گیاهی اخیراً برای درمان دیابت مورد توجه قرار گرفته اند. یکی از عوارض دیابت پوکی استخوان بوده و خطر شکستگی استخوان را در بیماران دیابتی افزایش می‌دهد. گیاه گلدر با داشتن خصوصیات آنتی اکسیدانی و کاهش دهنده قند خون به عنوان عامل ضد دیابتی معرفی شده است. هدف: این مطالعه بررسی اثرات بالقوه محافظتی بر بافت استخوان توسط عصاره الکلی گیاه گلدر در موش‌های دیابتی می‌باشد. روش‌کار:‌ ‌40 سر موش نر ویستار به 5 گروه مساوی تقسیم شدند. گروه 1 سالم ولی گروه‌های 2، 3، 4 و5 با تزریق داروی استرپتوزوتوسین با دوز ‌mg/kg50 دیابتی شدند. گروه 1و 2 تنها حلال عصاره و بقیه به ترتیب دوزهای ‌mg/kg‌ 200، 300 و 450 از عصاره را به مدت 28 روز دریافت کردند. در روز 29 با عمیق کردن بیهوشی، حیوانات کشته شدند. سرم برای تعیین گلوکز و استخوان‌های ران، تیبیا–فیبولای چپ برای مطالعه بافتی برداشت و مهره 4 کمری برای اندازه‌گیری خاکستر برداشت شد. داده‌ها با نرم افزار ‌SPSS‌ و آزمون آنالیز واریانس و آزمون تعقیبی توکی مقایسه و تجزیه و تحلیل شد. نتـایج: تجویز دوز ‌mg/kg‌ 300 باعث کاهش قند خون گردید. پارامترهای قطر ترابکول‌های اپی فیزیال و متا فیزیال، مساحت بافت به مساحت استخـوان در گـروه‌هـای دیـابتـی کاهش نشان داد ولی دوز ‌mg/kg‌ 200 باعث جلوگیری از این کاهش گردید. وزن خاکستر مهره 4 کمری در دوز ‌mg/kg200 مشـابـه بـا گـروه سـالـم ولـی در بقیـه گـروه‌هـا کـاهـش داشـت. قـطـر استئـویـد بین گروه‌ها اختلاف معنی‌داری نداشت(05/0‌p<‌.) نتیجـه‌‌گیری‌نهایی:‌ ‌عصاره الکلی گلدر با دوز کم، در موش‌های دیابتی شده توسط استرپتوزوتوسین دارای اثرات محافظ بر استخوان اسفنجی می‌باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Bone protective effects following ethanolic extract (Otostegia persica) administration in streptozotocin-induced diabetic rats: histomorphometric study

نویسندگان [English]

  • Maryam Rezaeian 1
  • Aydin Dilmaghanian 1
  • Tahoora Shomali 2
  • Masoud Adibmoradi 1
  • Ali Rasooli 3
1 Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran,Tehran-Iran
2 Department of Pharmacology, School of Veterinary Medicine, University of Shiraz, Shiraz-Iran
3 Department of Pharmacology, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran
چکیده [English]

BACKGROUND: Medical plants have been recently used to treat diabetes. Osteoporosis is one of diabetes side effects and increases the risk of bone fracture in diabetic patient. OBJECTIVES: The purpose of the present study was to investigate the potential bone protective effects of O.persica ethanolic extract in streptozotocin-induced diabetic rats. METHODS: Forty male rats were randomly divided into five equal groups and treated as follows: group 1 (control); group 2 (STZ group): received STZ 50 mg/kg by a single IP injection; groups 3, 4 and 5 treated with STZ as above+ 200 mg/kg, 300 mg/kg and 450 mg/kg of O. persica extract per day by oral gavage, respectively. On day 29, serum taken for glucose level measurement and left femoral and tibio-fibular bones were dissected for histomorphometric study, while L4 vertebrate were removed for determination of ash weight. RESULTS: 300mg/kg of extract reduced serum glucose levels. Epiphyseal and metaphyseal Trabecular thickness as well as epiphyseal bone area/tissue area significantly decreased in STZ group. O. persica extract at the dosage of 200 mg/kg reversed all these parameters to the control level. No significant difference observed in osteoid thickness among different groups. Ash weight of L4 vertebrate in rats treated with 300 and 450 mg/kg of extract was significantly lower than other groups. CONCLUSIONS: The results show that ethanolic extract of O. persica has bone protective effects in STZ-treated rats.

کلیدواژه‌ها [English]

  • bone loss
  • Diabetes mellitus
  • Otostegia persica
  • rats
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