بررسی کیفیت اسپرم در موشهای مواجهه شده با کافور و نقش محافظتی ویتامینE

نویسندگان

گروه علوم پایه، دانشکده دامپزشکی دانشگاه تهران، تهران- ایران

چکیده

زمینه مطالعه: در طب سنتی کشورهای آسیایی از جمله ایران، این تصور وجود دارد که کافور می‌تواند در کاهش میل جنسی افراد نقش داشته باشد. تا به امروز هنوز صحّت این باور به اثبات نرسیده است همچنین مطالعات در این زمینه نیز بسیار محدود می‌باشد. هدف: در تحقیق حاضر علاوه بر بررسی دقیق‌تر اثرات کافور بر کیفیت اسپرم‌ موش سوری، نقش ویتامین E بعنوان یک آنتی‌اکسیدانت در درمان عوارض ناشی از کافور بررسی شد. روش‌کار: در این تحقیق از30 قطعه موش سوری نر بالغ (balb/c) 25- 20 گرمی در 5 گروه مورد استفاده قرار گرفتند.
گروه اول کنترل (CO)، 
دریافت‌کننده سرم فیزیولوژی، گروه 2 و 3 بعنوان کنترل شم، به ترتیب دریافت‌کننده روغن زیتون تنها (OL) و گروه دریافت‌کننده ترکیبی از ویتامین E و روغن زیتون (OL+E)، و نهایتاً دو گروه تجربی دریافت‌کننده کافور تنها (CA) و گروه دریافت‌کننده کافور به‌همراه ویتامین Eر(CA+E). کافور با دوز mg/kg‌30 و ویتامین E نیز با دوز mg/kg‌100 و بصورت روزانه تهیه شدند. تمام مواد به صورت خوراکی (گاواژ) تجویز شدند. بعد از اتمام دوره درمان 35 روزه، نمونه اسپرم از قسمت دم اپی‌دیدیم اخذ و از نظر شمارش کلی و بررسی میزان تحرک، میزان زنده بودن، بلوغ هسته و آسیب DNA مورد ارزیابی قرار گرفتند. نتایج: نتایج نشان دهنده کاهش
معنی‌دار تعداد اسپرم‌ها، درصد اسپرم‌های زنده، افزایش در میزان اسپرم‌های نابالغ و عدم تغییر در میزان تحرک و آسیب DNA اسپرم در گروه دریافت-کننده کافور بود. ویتامین E بعنوان یک آنتی‌اکسیدانت توانسته بود اثرات کافور بر میزان زنده مانی و بلوغ اسپرم‌ها را کاهش دهد (05/0>p). نتیجه‌گیری نهایی: می‌توان نتیجه گرفت که کافور بر پارامترهای کیفیت اسپرم در موش سوری تأثیر داشته و ویتامین E بعنوان یک آنتی‌اکسیدانت، تا حدی عوارض ناشی از دریافت کافور در رابطه با کیفیت اسپرم را می‎تواند تعدیل کند.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of sperm quality in mice exposed to camphor and protective role of Vitamin E

نویسندگان [English]

  • masood adibmoradi
  • ali kalantari hesari
  • Hassan morovvati
  • farzad asadi
  • Hamid Reza Moradi
Department of Basic Sciences, Faculty of Veterinary Medicine, Tehran University, Tehran- Iran
چکیده [English]

BACKGROUND: In traditional medicine in some Asian countries, including Iran, there is a belief that camphor is a suppressor of sexual activity. Not only has the validity of this hypothesis not been established, but also studies in this field are very limited.  OBJECTIVES: In this study, we investigated the effects of camphor on sperm quality in mice, and to protect sperm damage vitamin E as an antioxidant was used. METHODS: This study was conducted on 30 adult male mice (balb/c) with weight range 20-25 gr in 5 groups. First group was control (CO) and treated with normal saline,  groups  2 and 3 were sham groups treated respectively with Olive oil (OL) and the combination of olive oil and vitamin E (OL+E), and finally, two experimental groups were treated using camphor (CA) and camphor with vitamin E (CA+E). Camphor at doses of 30 mg/kg/day and vitamin E at doses of 100 mg/kg/day were prepared. All materials were administered orally (gavage). After 35 days semen were collected from tail of epididymis, and then total count, motility, viability, nuclear maturity, and DNA damage were examined. RESULTS: Results showed significant reduction in sperm total count, percentage of viability, increase in  the number of immature sperms and  no significant difference in rate of motile sperms and sperms with damaged DNA in groups that received Camphor was observed. Vitamin E as a strong antioxidant administered lightly was able to reduce the effects of  Camphor on viable and mature sperms (p<0.05). CONCLUSIONS: It can be concluded that Camphor could affect on mice sperm quality and vitamin E as an antioxidant,  was able to slightly reduce Camphor effects in sperm quality.

کلیدواژه‌ها [English]

  • Sperm
  • mice
  • camphor
  • vitamin E

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