مقایسه روشهای درمانی شامل: شیمی درمانی با دوکسوروبیسین، اولتراسونوگرافی 150KHzو ترکیب این دو روش در ایجاد مناطق نکروزه با روش هیستوپاتولوژی درتومور ادنوکارسینومای پستان موش

نویسندگان

1 بخشپاتولوژی،دانشکدهدامپزشکیدانشگاهتهران،تهران،ایران

2 بخشفیزیکپزشکی،دانشکدهفیزیکپزشکیوفیزیولوژی،دانشگاهعلومپزشکیاراک،اراک،ایران

3 بخشپاتولوژی،دانشکدهدامپزشکیدانشگاهسمنان،سمنان،ایران

چکیده

زمینه مطالعه: سرطان پستان به تنهایی 28% از همه موارد سرطان را در میان زنان تشکیل داده است. اگرچه شیمی درمانی نقش بسیار مهمی در درمان سرطان دارد،با این حال استفاده ازداروهای ضد سرطان اثر نامطلوب خود را بر روی بافت نرمال هم می‌گذارد. لذا همواره دانشمندان به دنبال روشی برای کاهش دوز مصرفی این داروها بوده اند. هدف: این مطالعه با هدف بررسی اثرهم افزایی امواج فراصوت kHz 150 با شدت کم در ترکیب با داروی شیمی درمانی دوکسوروبیسین با دوز کاهش یافته در درمان رایج ترین سرطان پستان (ادنوکارسینوما) انجام شد. روش کار: در این مطالعه  25 موش ماده نژاد BALB/c انتخاب وتومور ادنوکارسینوما با روش جراحی در زیر پوست پستان آن‌ها کاشته شد. موش‌های دارای تومور به پنج گروه شامل شاهد، شم، تزریق وریدی دوکسوروبیسین (mg/kg 2)،امواج فراصوت (150kHz) و امواج + دارو (در غلظت 1mg/kg) تقسیم و تحت درمان قرارگرفتند. نتایج درمان تومور بین گروه‌ها با استفاده از آنالیز ANOVA در محیط نرم افزار نسخه 13 SPSS و تست تکمیلی توکی مورد بررسی قرار گرفت. نتایج: پس از تزریق دوکسوروبیسین و قرار گرفتن در معرض امواج فراصوت به مدت 15 دقیقه مناطق نکروزه در تومور ادنوکارسینوم در مقایسه با گروه کنترل وشم افزایش معنی‌داری را نشان داد (0.001>p). همچنین در مقایسه بین گروه دارو + امواج با گروهی که تنها دارو دریافت کرده بودند گروه دریافت کننده امواج رشد مناطق نکروزه بیشتری را نسبت به حجم کل تومور نشان داد (0.05>p). نتیجه‌گیری‌نهایی: نتایج این مطالعه حاکی از این است که استفاده ازامواج فراصوت با شدت کم می‌تواند در کاهش دوز داروی دوکسوروبیسین در درمان ادنوکارسینومای پستانی مؤثر واقع گردد.

کلیدواژه‌ها

عنوان مقاله [English]

Histopathological findings in necrotic spaces developed with doxorubicin and 150 kHz ultrasound at low intensity and a combination of these two methods on adenocarcinoma tumor breast cancer in BALB/c mice

نویسندگان [English]

  • Sahar Ghaffari Khaligh 1
  • abas tavasoli 2
  • Seyed Hossein Marjanmehr 1
  • Homa Soleimani 2
  • Abbas Javaheri Vayghan 3
1 Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2 Department of Medical Physics and Physiology, School of Medical Science, Arak university of Medical Science, Arak, Iran
3 Department of Pathology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran
چکیده [English]

BACKGROUND: Breast cancer is the most commonly diagnosed cancer in women. One in eight women will be diagnosed with breast cancer in their lifetime. Chemotherapy works on active cells. Active cells are cells that are growing and dividing into more of the same type of cell. Cancer cells are active, but so are some healthy cells. Also, scientists work constantly to develop ways of providing treatment with fewer chemotherapy side effects. Objectives: The aim of this study was antitumor effect of simultaneous low-intensity, 150 kHz ultrasound, in combination with the reduced dose of anticancer drug Doxorubicin (DOX) on breast adenocarcinoma using murine model (BALB/c). Methods: Twenty-five female BALB/c mice were used in this study. The tumor was implanted under the breast skin of mice. Mice were divided into five groups, namely control, sham, drug (IV injected of 2 mg/kg of DOX), drug (IV injected of 1 mg/kg of DOX) + US (150 kHz for 15 minutes) and exposure to ultrasound (150 kHz for 15 minutes) alone. The data were analyzed employing ANOVA using SPSS software V.13 and complementary test of Tooki was done. Results: It was shown that, after injection of DOX, exposure to ultrasound at 150 kHz the necrotic spaces in adenocarcinoma tumors compared to control and sham groups have meaningful variance (p<0.001). There was also a significant difference (the bigger the necrotic spaces) between the drug+US group and drug treated group (p<0.05), It should be mentioned that the dose of DOX in drug+US group was reduced to 1mg/kg. Conclusions: The co-administration of DOX and low-intensity ultrasound provided a more effective treatment than the drug alone in murine adenocarcinoma breast cancer. The combined treatment appeared to produce synergistic effects that could prove potentially useful in reducing the side effects of DOX by lowering the required effective dose of the drug while increasing the efficiency of the therapy as a whole.

کلیدواژه‌ها [English]

  • Breast Cancer
  • doxorubicin
  • Ultrasound
  • necrotic space
  • acoustic cavitation

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