مطالعه میزان فراوانی آلودگی شغال های طلایی به سالمونلا با استفاده از روش های کشت میکروبی و PCR در استان های گلستان و مازندران

نویسندگان

1 دانشگاه علوم کشاورزی و منابع طبیعی گرگان دانشکده شیلات و محیط زیست گروه محیط زیست

2 Department of Microbiology, Faculty of Veterinary Medicine, University of Semnan Semnan, Iran

3 Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran

4 دانشگاه علوم کشاورزی و منابع طبیعی گرگان

چکیده

مقدمه: سالمونلاها از جمله پاتوژن های زئونوز بوده و طیف وسیعی از حیوانات اهلی و وحشی را آلوده می کنند. گوشتخواران فرصت طلب و همه چیز خوار چون شغال طلایی که معمولاً با تعداد بالا در نواحی روستایی پرسه می زنند، می توانند به عنوان منبع بالقوه آلودگی به سالمونلا برای انسانها و سایر حیوانات اهلی و وحشی در شمال ایران عمل کنند.
هدف: هدف از انجام این مطالعه بررسی آلودگی شغال ها به سالمونلا و الگوی مقاومت آنتی بیوتیکی جدایه ها در استانهای گلستان و مازندران بود.
مواد و روش کار: در سالهای 1393 تا 1394 نمونه مدفوع 50 شغال تلف شده بر اثر تصادف با وسایل نقلیه جمع آوری و با استفاده از روش کشت میکروبی و PCR جهت بررسی آلودگی به سالمونلا مورد بررسی قرار گرفت و متعاقباً سروتیپ و الگوی مقاومت باکتریایی جدایه ها مشخص گردید.
نتایج: با استفاده از روش کشت و PCR، 5 نمونه مبتلا به سالمونلا (10%) که متعلق به 2 سروتیپ،(5/3) S typhymurium و (5/2) S arizona بودند، در نمونه های مدفوع مورد شناسایی قرار گرفت. میزان آلودگی به سالمونلا در دو جنس نر و ماده مشابه بود و بیشترین میزان آلودگی در شغال های زیر 2 سال دیده شد.
2 جدایه S typhymurium و 1 جدایه S arizona مقاومت آنتی بیوتیکی بالایی نسبت به آنتی بیوتیک های استرپتومایسین و آمپی سیلین نشان دادند.
بحث و نتیجه گیری: آلودگی 10 درصدی شغال های طلایی مورد مطالعه، بیانگر نقش فراموش شده این گونه در انتشار بیماری های مشترک و در معرض خطر قرار دادن سلامتی انسان ها در نواحی روستایی استان گلستان و مازندران می باشد. انجام مطالعات اپیدمیولوژیک جهت بررسی نقش حیوانات وحشی در انتشار سالمونلا و همچنین اجرای برنامه های مناسب جهت پیشگیری و کنترل سالمونلوز ضروری بنظر می رسد.

کلیدواژه‌ها


عنوان مقاله [English]

Survey on Salmonella contamination of Golden Jackals by microbiological culture methods and PCR in Golestan and Mazandaran Provinces

نویسندگان [English]

  • somayeh namroodi 1
  • seyyed vahhab sharafi 4
4 gorgan university of agricultural culture and natural resources
چکیده [English]

Introduction and objective: Salmonella spp. are zoonotic pathogens have been infected a wide range of domestic and wild animals. Opportunistic wild carnivores such as Golden jackal (Canis aureus) which stray in high numbers around the rural areas can act as potential sources of salmonella spp in humans and wild & domestic animals in North Iran.
The object of this survey was to examine the Salmonella spp infection including the antibiotic-resistant pattern in golden jackals in Golestan and Mazandaran Province.
Material and Methods: Between 2013 and 2015, fecal samples of 50 road-killed Golden jackals (Canis aureus), were collected and analyzed for Salmonella contamination by classical microbiological culture methods and PCR followed by serotyping and determining of antibiotic resistant pattern.
Results: 5 Salmonella belonging to 2 serotypes: S typhymurium (3/5) and S arizona (2/5) were isolated by culturing and PCR. The rate of Salmonella contamination was similar between females and males and higher incidence detected in jackals under 2 years old.
Conclusion: 10% Salmonella infection of sampled golden jackals highlights the neglected role of this species in zoonotic diseases dissemination and posing a great threat to human health in rural areas of Golestan and Mazandaran Provinces.
The epidemiological study on role of wild animals in the spread of salmonella and developing strategy for salmonellosis prevention and control seems necessary.

کلیدواژه‌ها [English]

  • Golden jackals
  • Salmonella
  • PCR
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