بررسی تغییرات هورمونی، پاسخ ایمنی و زمان بازگشت به تولید در مرغان تخمگذار تغذیه شده با جیره تولک حاوی پودر یونجه

نویسندگان

1 گروه علوم دامی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان قزوین، قزوین، ایران

2 سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

چکیده

زمینه مطالعه: استفاده از روش گرسنگی می تواند سبب وارد شدن استرس شدید فیزیولوژیک و تاثیرات نامطلوب بر روی عملکرد سیستم ایمنی پرنده شده و عملکرد پس از تولک را تحت تاثیر قرار دهد. هدف: هدف از انجام این تحقیق بررسی تغییرات غلظت هورمون های کورتیکو استرون و تیروئید و همچنین پاسخ ایمنی در خلال دوره تولک در مرغان تخمگذار تعذیه شده با جیره تولک حاوی پودر یونجه بود. روش کار: 108 قطعه مرغ تخمگذار در قالب یک طرح کاملاً تصادفی با 3 تیمار و 6 تکرار انتخاب شدند. تیمارهای مورد استفاده در این آزمایش جهت اعمال تولک بری پرنده‌ها به مدت 12 روز شامل: 1- گروه شاهد تغذیه شده با جیره تخمگذاری (FF) 2- گروه محروم از غذا (FW)، 3- گروه تغذیه شده با جیره تولک حاوی 90 درصد پودر یونجه به همراه 10 درصد جیره تخمگذاری (A90)، بودند. نتایج: نتایج نشان دادند که جمعیت کل سلول‌های سفید خون در گروه FW در مقایسه با سایر گروه‌ها پایین تر بود (05/0p<). نسبت هتروفیل به لنفوسیت در روزهای سوم و ششم تولک در گروه FW بیشترین افزایش را نشان داد (05/0p<). غلظت هورمون کورتیکواسترون در روز سوم تولک در گروه FW بالاتر از گروه A90 بود (05/0p<). غلظت هورمون T3 در خلال دوره تولک در مرغ‌های تولک رفته در مقایسه با گروه شاهد به طور معنی‌داری کاهش یافت (05/0p<). غلظت هورمون T4 در خلال دوره تولک در مرغ‌های تولک رفته در مقایسه با گروه شاهد افزایش یافت (05/0p<). زمان بازگشت به تولید و زمان رسیدن به 50 و 80 درصد تولید در گروه A90 در مقایسه با گروه FW به طور معنی‌داری کوتاهتر بود (05/0p<). نتیجه گیری نهایی: پودر یونجه ضمن ایجاد عملکرد مطلوب در تولک‌بری سبب کاهش استرس فیزیولوژیک و عملکرد مناسبتر پاسخ ایمنی نسبت به روش گرسنگی، در جریان تولک بری گردید.

کلیدواژه‌ها

عنوان مقاله [English]

Hormonal Changes, Immune Response and Date of Reentry in Laying Hens Fed by Alfalfa Molt Diet

نویسندگان [English]

  • Ehsan Shahrami 1
  • Hasan Rokni 2
1 1Department of Animal Sciences, Qazvin Agricultural and Natural Resources Research and Education Center, Qazvin, Iran
2 Agricultural Research, Education and Extension Organization, Tehran-Iran
چکیده [English]

BACKROUND: The use of feed withdrawal method for molt induction can be caused physiological stress and negatively affected the immunological response of hens. OBJECTIVES: The aim of the present study was to survey of hormonal changes and immunological response in hens fed by alfalfa molt diet during the molt period. METHODS: In this experiment 108 Hy-line (W36) laying hens aged 74 weeks in a completely randomized design with 3 treatments and 6 replicates were used. The using treatments for 12 days molt period were included: 1- control group fed with layer ration (FF), 2- feed withdrawal group (FW), 3- group fed with 90% alfalfa and 10% layer ration (A90). Performance of birds was monitored for 12 weeks after the end of the molting period. RESULTS: Total circulating leukocyte were generally lower in FW hens group compared with the other groups during the initial stage of molt (P<0.05). On d 3 and 6 of molt, heterophil to lymphocyte ratio were incresed in molted hens and FW hens had higher levels than other groups. On d 3 of molt, plasma corticosterone was generally incresed in molted hens and FW hen higher level than A90 hens (P<0.05). Plasma T3 was significantly decreased in molted hens than nonmolted hens during the molt period (P<0.05). Plasma T4 was significantly increased in molted hens than nonmolted hens during the molt period (P<0.05). On d 6 and 9 of molt, concentrations of T4 were higher in FW hens than in the A90 hens (P<0.05). Date of reentry and days return to 50% and 80% egg production were significantly lower in A90 groups than in the FW groups (P<0.05). CONCLUSIONS: The results showed that, A90 diet can limit physiological stress and improve the performance of immunological response that accompany feed withdrawal method during an induced molt.

کلیدواژه‌ها [English]

  • Alfalfa
  • corticosterone
  • Forced molting
  • laying hens
  • Thyroid hormons

مراجع

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