معرفی و استفاده از تست فاماچا جهت مدیریت درمان و انتخاب دام‌های مقاوم به نماتدهای دستگاه گوارش در گوسفند

نویسندگان

1 گروه علوم دامی، دانشکده دامپزشکی دانشگاه تبریز، تبریز، ایران

2 گروه پاتوبیولوژی، دانشکده دامپزشکی دانشگاه تبریز، تبریز، ایران

چکیده

چکیده
زمینه مطالعه: نماتدهای موجود در دستگاه گوارش باعث ایجاد مشکلات گوارشی، بروز کم‌خونی و کاهش وزن در گوسفند و بز می‌شوند. هدف: هدف از این مطالعه معرفی و استفاده از تست فاماچا (FAMACHA test) در تشخیص اولیه‌ی دام‌های آلوده به نماتدهای دستگاه گوارش (بویژه انگل همونکوس‌کنتورتوس Haemonchus contortus) در گوسفند جهت کاهش جمعیت تحت درمان با داروی ضد‌انگل و انتخاب دام‌های مقاوم می‌باشد. روش کار: در این مطالعه از 120 بره‌ی 4 تا 6 ماهه‌ی نژاد قزل در 6 گله‌ی متفاوت 20‌راسی‌ استفاده شده که بطور‌تصادفی از گله‌های‌ مختلف مردمی مرتع‌رو انتخاب شده بودند. نمونه‌برداری‌ها و تست فاماچا در سال 1392 انجام شده و نمونه‌ی خونی جهت اندازه‌گیری‌ درصد حجم سلول‌های فشرده شده‌ی خونی، نمونه‌ی مدفوعی جهت اندازه‌گیری تعداد تخم‌انگل شمارش شده در هر گرم مدفوع و وزن بدن مورد بررسی قرار گرفتند. نمونه-گیری‌ها 2‌بار و با فاصله‌ی یک هفته‌ای انجام شدند. نتایج: نتایج نشان داد که اثر امتیازات تست فاماچا، اثر گله و اثر متقابل گله و تست فاماچا روی تعداد تخم‌انگل شمارش شده در هر گرم مدفوع معنی‌دار بود (01/0p<) و همینطور اثر تست فاماچا بر‌روی‌ درصد حجم سلول‌های فشرده شده‌ی خونی (PCV%) نیز معنی‌دار بود (001/0p<). نتیجه‌گیری نهایی: بنظر می‌رسد تست فاماچا در ایران می‌تواند بعنوان یک روش کمکی و جایگزین برای تشخیص اولیه آلودگی به نماتد‌های خونخوار و پیشگیری از بروز موارد جدید مقاومت دارویی و انتخاب دام‌های مقاوم بکار برده شود.
کلمات کلیدی: تست فاماچا ، انگل همونکوس‌کنتورتوس، همونکوزیس، گوسفند.

کلیدواژه‌ها


عنوان مقاله [English]

Introduce and usage of FAMACHA test for treatment management and selection of resistant animal to gastrointestinal nematodes in sheep

نویسندگان [English]

  • Rahman Hajializadeh Valilou 1
  • Seyed Abbas Rafat 1
  • Ahmad Nematollahi 2
1 1Department of Animal Science, University of Tabriz, Tabriz, Iran
2 2Department of Pathobiology, Veterinary College, University of Tabriz, Tabriz, Iran
چکیده [English]

Abstract
BACKGROUND: Gastrointestinal nematodes causing digestive problems, anemia and weight loss in sheep and goat. OBJECTIVES: The aim of this study is to introduce FAMACHA test and usage of it for early detection of infected animals with gastrointestinal nematodes (especially Haemonchus contortus) to reducing of treated sheep population with anthelmintic drug and selection of resistant animals. METHODS: In this study 120 Ghezel sheep breed lambs were used in age of 4 to 6 months. The lambs were chosen randomly from 6 different grazing flocks with 20 lambs in each of them. Sampling and FAMACHA test scoring was carried out in 2014. Blood samples was collected for calculating of packed cell volume percent and fecal sampling was collected for counting of egg per each grams of fecal and body weight was investigate. The sampling was carried out 2 times with a week apart. RESULTS: Results showed that the effects of FAMACHA, flocks and interaction between them on parasite eggs per gram of fecal was significant (p<0.01) and also the effect of FAMACHA on the percentage of packed cells volume was significant (p<0.01). CONCLUSIONS: It seems that the FAMACHA test scoring could be used as a synergetic and alternative method to early diagnosis of infestation to blood sucking nematodes and prevention of new cases of drug resistant and selection of resistant animals in Iran.

Key words: FAMACHA test, Haemonchus contorts parasite, Haemonchosis, sheep

کلیدواژه‌ها [English]

  • Key words: FAMACHA test
  • Haemonchus contorts parasite
  • Haemonchosis
  • sheep
 

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