تأثیر عصاره زنجبیل (Zingiber officinale) بر برخی شاخصهای رشد، خون، بیوشیمی و ایمنی ماهی قزلآلای رنگینکمان (Oncorhynchus mykiss)

نوع مقاله: بهداشت خوراک دام، طیور و آبزیان

نویسندگان

گروه شیلات، واحد آزادشهر، دانشگاه آزاد اسلامی، آزادشهر، ایران

چکیده

 
زمینه مطالعه: جایگزنی مواد طبیعی بجای داروهای شیمیایی به منظور افزایش تولید و ایمنی. هدف: هدف از این مطالعه بررسی تجویز خوراکی عصاره زنجبیل بعنوان محرک ایمنی طبیعی بر برخی شاخص‌های رشد، خون، بیوشیمی و ایمنی ماهی قزل‌آلای رنگین کمان بود. روشکار: بدین منظور ماهیان با میانگین وزنی g ۲/0±1/14 به روش خوراکی و با سطوح  5/0% و 1% عصاره هیدروالکلی زنجبیل به مدت 8 هفته تغذیه شدند. در پایان دوره تغذیه از ورید ساقه دمی ماهیان خونگیری شد. شاخص‌های رشد (وزن نهایی، نرخ رشد ویژه، فاکتور وضعیت)، خون (گلبول سفید، گلبول قرمز، هماتوکریت، هموگلوبین، لنفوسیت، مونوست، نوتروفیل)، بیوشیمی (پروتئین، آلبومین، کلسترول، تری گلیسرید، گلوکز، کورتیزول، آنزیم آلانین آمینوترانسفراز (ALT)، آسپارتات آمینوترانسفراز (AST)، لاکتات دهیدروژناز (LDH)، آلکالین فسفاتاز (ALP) و ایمنی (لیزوزیم، ایمنوگلبولین کل، سوپراکسیددسموتاز، فعالیت مسیر جانبی کمپلمان) مورد سنجش قرار گرفتند. گروه شاهد نیز بدون استفاده از عصاره گیاهی و با شرایط یکسان با سایر تیمارها مورد آزمایش قرار گرفت. نتایج: نتایج مطالعه حاضر تفاوت معنی‌داری در شاخص‌های رشد، خون، بیوشیمی و آنزیم‌های سرمی بین تیمارهای حاوی عصاره زنجبیل در مقایسه با گروه شاهد نشان نداد (05/0<p). کمترین میزان کورتیزول بطور معنی‌داری در تیمار 5/0% عصاره زنجبیل بدست آمد (05/0>p). افزایش معنی‌داری در فعالیت لیزوزیم سرم در تیمار 5/0% عصاره زنجبیل مشاهده شد (05/0>p). نتیجهگیرینهایی: تجویز خوراکی عصاره زنجبیل در سطح 5/0%  می‌تواند بعنوان محرک ایمنی طبیعی جهت بهبود پارامترهای رشد و ایمنی ماهی قزل‌آلا مورد استفاده قرار گیرد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of feed supplemented with ginger (Zingiber officinale) extract on the growth, biochemical and hemato-immunological parameters of rainbow trout (Oncorhynchus mykiss)

نویسندگان [English]

  • Reza Akrami
  • Zeid Ahmadi
  • Mahshid Shamloo
  • Farzaneh ahaabibi Nodeh
  • Fatemah Sadeghi Asl
  • Nazanin Zarrini
  • Hosein Chitsaz
Department of Fisheries, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran
چکیده [English]

BACKGROUND: Replacement of natural materials with synthetic
drugs in order to increase production and safety. OBJECTIVE: The purpose
of this study was to investigate the effect of feed supplemented with ginger (Zingiber
officinale
) extract on the growth, biochemical and hemato-immunological
parameters of rainbow trout (Oncorhynchus mykiss). METHODS: Fish
with an average body weight 14.1±0.2 g were fed diet for 8 weeks with 0.5% and
1% ginger extract and with unsupplemented commercial diet as the control. Blood
samples were collected from caudal vein from apparently healthy fish at the end
of trial. Growth (weight gain, specific growth ratio and condition factor),
hematological (RBC,WBC, Hb, Hct, monocyte, lymphocyte and neutrophil),
Biochemical (glucose, total protein, triglycerides, cholesterol, albumin, AST,
ALT, LDH and ALP) and immunological (lysozym activity, ACH50,IgM, and SOD)
parameters were determined. RESULTS: The results showed that there were
no significant differences (p>0.05) in growth, hematological, biochemical
and metabolic enzymes between fish fed control and ginger extract
supplementation. The lowest level of cortisol was observed in 0.5% ginger
extract (p<0.05). Lysozyme activity was significantly increased in 0.5%
ginger extract fed fish (p<0.05). CONCLUSIONS: The results suggest
that by using 0.5% ginger extract there will be an improvement in growth and
immune function of rainbow trout

کلیدواژه‌ها [English]

  • Ginger (Zingiber officinale) extract
  • Growth
  • Blood variable
  • immune response
  • Rainbow trout
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